动物营养学报 >

2022 , Vol. 34 >Issue 9: 5702 - 5712

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.09.025

研究论文 RESEARCH PAPER

ε-聚赖氨酸对黄羽肉鸡生长性能、肉品质、血清生化指标和肠道健康的影响

  • 郭燕珊 ,
  • 邹晨浩 ,
  • 赵陆原 ,
  • 曹久爱 ,
  • 葛超悦 ,
  • 罗鑫宇 ,
  • 刘莉君 ,
  • 占秀安 ,
  • 陈少杰 ,
  • 刘兵 ,
  • 余东游
展开
  • 1. 浙江大学海南研究院, 三亚 572025;
    2. 浙江大学动物科学学院, 杭州 310058;
    3. 浙江省畜牧技术推广与种畜禽监测总站, 杭州 310029;
    4. 浙江新银象生物工程有限公司, 台州 317200
郭燕珊(1997—),女,广东广州人,硕士研究生,从事微量养分营养研究。E-mail:22017076@zju.edu.cn

收稿日期: 2022-03-03

  网络出版日期: 2022-10-17

基金资助

浙江省农业重大技术协同推广计划项目（2021XTTGXM04）

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Effects of ε-Polylysine on Growth Performance, Meat Quality, Serum Biochemical Indices and Intestinal Health of Yellow Feathered Broilers

  • GUO Yanshan ,
  • ZOU Chenhao ,
  • ZHAO Luyuan ,
  • CAO Jiu'ai ,
  • GE Chaoyue ,
  • LUO Xinyu ,
  • LIU Lijun ,
  • ZHAN Xiu'an ,
  • CHEN Shaojie ,
  • LIU Bing ,
  • YU Dongyou
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  • 1. Hainan Institute of Zhejiang University, Sanya 572025, China;
    2. College of Animal Science, Zhejiang University, Hangzhou 310058, China;
    3. Zhejiang Provincial Animal Husbandry Technology Extension and Monitoring Station of Breeding Livestock and Poultry, Hangzhou 310029, China;
    4. Zhejiang Silver-Elephant Bio-Engineering Co., Ltd., Taizhou 317200, China

Received date: 2022-03-03

  Online published: 2022-10-17

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摘要

本试验旨在研究饲粮中添加ε-聚赖氨酸（ε-PL）对肉鸡生长性能、肉品质、血清生化指标、肠道黏膜形态和氮代谢率的影响，以评价ε-PL在肉鸡生产中的应用效果。试验选用640羽健康且体重相近的1日龄雄性黄羽肉鸡，随机分为4个组，每组8个重复，每个重复20羽。空白对照组（CON组）肉鸡饲喂正常赖氨酸水平的基础饲粮，苯甲酸钠组（SB组）肉鸡饲喂在基础饲粮中添加500 mg/kg苯甲酸钠的试验饲粮，ε-PL组肉鸡饲喂在基础饲粮中分别添加250（ε-PL1组）和500 mg/kg （ε-PL2组）的ε-PL的试验饲粮。试验期为10周。结果表明：1）与CON组和SB组相比，ε-PL1组和ε-PL2组肉鸡平均日增重显著提高（P<0.05），料重比显著降低（P<0.05）；饲粮中添加ε-PL对肉品质无显著影响（P>0.05）。2）与CON组和SB组相比，饲粮中添加ε-PL显著提高了血清总蛋白含量（P<0.05），显著降低了血清尿酸含量（P<0.05）。3）与CON组和SB组相比，ε-PL1组35和70日龄肉鸡回肠绒毛高度显著提高（P<0.05），ε-PL2组70日龄肉鸡回肠绒毛高度显著提高（P<0.05）。综上所述，饲粮中添加250和500 mg/kg的ε-PL可改善肉鸡肠道黏膜形态，增强肉鸡蛋白质的合成代谢，进而提高肉鸡的生长性能，且添加250 mg/kg的ε-PL效果最佳。

关键词： ε-聚赖氨酸; 生长性能; 肉品质; 血清生化指标; 氮代谢率; 肉鸡

本文引用格式

郭燕珊 , 邹晨浩 , 赵陆原 , 曹久爱 , 葛超悦 , 罗鑫宇 , 刘莉君 , 占秀安 , 陈少杰 , 刘兵 , 余东游 . ε-聚赖氨酸对黄羽肉鸡生长性能、肉品质、血清生化指标和肠道健康的影响[J]. 动物营养学报, 2022 , 34(9) : 5702 -5712 . DOI: 10.3969/j.issn.1006-267x.2022.09.025

Abstract

This study was conducted to investigate the effects of dietary ε-polylysine (ε-PL) supplementation on growth performance, meat quality, serum biochemical indices, intestinal mucosa morphology and nitrogen metabolism of yellow feather broilers. A total of 640 one-day-old healthy male yellow feather broilers with similar weight were assigned to 4 groups with 8 replicates of 20 broilers. Broilers in control group (CON group) were fed a basal diet with normal lysine level, broilers in the preservative control group (SB group) were fed the basal diet supplemented with 500 mg/kg sodium benzoate, and broilers in the experimental groups were fed basal diets supplemented with 250 (ε-PL1 group) and 500 mg/kg ε-polylysine (ε-PL2 group), respectively. The experiment lasted for 10 weeks. The results showed as follows:1) compared with CON and SB groups, the average daily gain (ADG) of broilers in ε-PL1 and ε-PL2 groups significantly increased (P<0.05), and feed/gain ratio significantly decreased (P<0.05). 2) Compared with CON and SB groups, adding ε-PL in diets significantly increased the serum total protein content (P<0.05), and decreased significantly uric acid in serum of broilers (P<0.05). 3) Compared with CON and SB groups, the ileal villous height of 35 and 70-day-old broilers in ε-PL1 group significantly increased (P<0.05), and the ileal villous height of 70-day-old broilers in ε-PL2 group significantly increased (P<0.05). In conclusion, dietary supplemented with 250 and 500 mg/kg ε-PL is beneficial to improve the intestinal mucosa morphology and the anabolism of protein, thus to improve the growth performance, and the optimal supplemental level of ε-PL in broilers' diets is 250 mg/kg.

Key words： ε-polylysine; growth performance; meat quality; serum biochemical indices; nitrogen metabolism; broilers

参考文献

[1] SHUKLA S C, SINGH A, PANDEY A K, et al.Review on production and medical applications of ε-polylysine[J].Biochemical Engineering Journal, 2012, 65:70-81.
[2] LI Y Q, HAN Q, FENG J L, et al.Antibacterial characteristics and mechanisms of ε-poly-lysine against Escherichia coli and Staphylococcus aureus[J].Food Control, 2014, 43:22-27.
[3] ISHII M, SHIRAI M, AIUTI M, et al.Three month treated orally in dietary toxicity study of polylysine powder in rat[J].The Clinical Report, 1993, 27:2013-2033.
[4] FUKUTOME A, KASHIMA M, AIUCHI M.A combined chronic toxicity and carcinogenicity study of polylysine powder in rats by peroral dietary administration[J].The Clinical Report, 1995, 29:1416-1434.
[5] ZHANG X L, HOU Z P, XU B Y, et al.Dietary supplementation of ε-polylysine beneficially affects ileal microbiota structure and function in Ningxiang pigs[J].Frontiers in Microbiology, 2020, 11:544097.
[6] HIRAKI J, ICHIKAWA T, NINOMIYA S, et al.Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food[J].Regulatory Toxicology and Pharmacology, 2003, 37(2):328-340.  
[7] 贾红敏, 韩冰, 刘向阳, 等.赖氨酸及其在鸡、猪营养上的研究进展[J].动物营养学报, 2020, 32(3):989-997. JIA H M, HAN B, LIU X Y, et al.Advances in lysine and its nutrition in chickens and pigs[J].Chinese Journal of Animal Nutrition, 2020, 32(3):989-997.(in Chinese)
[8] 李波, 郭强, 杨利, 等.饲粮中添加天蚕素抗菌肽对肉鸡生长性能的影响[J].中国家禽, 2010, 32(20):55-56. LI B, GUO Q, YANG L, et al.Effect of dietary cecropin on growth performance of broilers[J].China Poultry, 2010, 32(20):55-56.(in Chinese)
[9] 王莉, 陈晓, 王书全.天蚕素抗菌肽对817肉杂鸡生长性能及免疫功能的影响[J].中国畜牧兽医, 2017, 44(8):2354-2359. WANG L, CHEN X, WANG S Q.Effects of antimicrobial peptide on growth performance and immune function of 817 broiler hybrids[J].China Animal Husbandry & Veterinary Medicine, 2017, 44(8):2354-2359.(in Chinese)
[10] 宇晓军, 李丹丹, 王路义, 等.抗菌肽对育成期母貂生长性能、养分表观消化率及肠道菌群的影响[J].动物营养学报, 2022, 34(2):1194-1204. YU X J, LI D D, WANG L Y, et al.Effects of antimicrobial peptide on growth performance, nutrient apparent digestibilities and intestinal flora of growing female minks[J].Chinese Journal of Animal Nutrition, 2022, 34(2):1194-1204.(in Chinese)
[11] WANG J J, DOU X J, SONG J, et al.Antimicrobial peptides:promising alternatives in the post feeding antibiotic era[J].Medicinal Research Reviews, 2019, 39(3):831-859.  
[12] 王世祺, 赵浩然, 杜明芳, 等.棒晶束解离凹凸棒石对肉鸡生长性能、血清生化指标、抗氧化功能和肉品质的影响[J/OL].动物营养学报:1-13(2022-03-20)[2022-04-16].http://kns.cnki.net/kcms/detail/11.5461.S.20220317.1336.098.html. WANG S Q, ZHAO H R, DU M F, et al.Effects of crystal-bundles disaggregated palygorskite on growth performance, serum biochemical indices, antioxidant function and meat quality of broilers[J/OL].Chinese Journal of Animal Nutrition:1-13(2022-03-20)[2022-04-16].http://kns.cnki.net/kcms/detail/11.5461.S.20220317.1336.098.html. (in Chinese)
[13] 仲伟静, 蒋宗勇, 邹书通, 等.鸡肉品质的营养调控研究进展[J].中国家禽, 2006, 28(20):83-87. ZHONG W J, JIANG Z Y, ZOU S T, et al.Nutritional manipulation of chicken meat quality:a review[J].China Poultry, 2006, 28(20):83-87.(in Chinese)
[14] RUSSELL S M, FLETCHER D L, COX N A.Spoilage bacteria of fresh broiler chicken carcasses[J].Poultry Science, 1995, 74(12):2041-2047.  
[15] 巨晓军, 刘一帆, 章明, 等.鸡肉品质性状评价指标与方法研究进展[J].中国家禽, 2019, 41(2):44-48. JU X J, LIU Y F, ZHANG M, et al.Research progress on evaluation indices and methods of chicken meat quality[J].China Poultry, 2019, 41(2):44-48.(in Chinese)
[16] 席鹏彬, 蒋守群, 蒋宗勇, 等.黄羽肉鸡肉质评定技术操作规程的建立[J].中国畜牧杂志, 2011, 47(1):72-76. XI P B, JIANG S Q, JIANG Z Y, et al.Establishment of technical regulation for evaluation of meat quality of yellow-feathered broilers[J].Chinese Journal of Animal Science, 2011, 47(1):72-76.(in Chinese)
[17] ALIREZALU K, MOVLAN H S, YAGHOUBI M, et al.ε-polylysine coating with stinging nettle extract for fresh beef preservation[J].Meat Science, 2021, 176:108474.
[18] SU Q L, LI B, LIANG J X, et al.Detection and analysis of routine blood indexes in Guike pig[J].Agricultural Biotechnology, 2018, 7(3):95-98, 103.
[19] 张磊.奶牛乳房炎的血液病理学研究[D].硕士学位论文.合肥:安徽农业大学, 2009. ZHANG L.The study of blood pathology of the cow mastitis[D].Master's Thesis.Hefei:Anhui Agricultural University, 2009.(in Chinese)
[20] KALMENDAL R, TAUSON R.Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet[J].Poultry Science, 2012, 91(6):1387-1393.  
[21] 宫汝淳, 田小东, 欧小女, 等.桑黄多糖对育成期蛋鸡生长性能、血液生化及免疫相关指标的影响[J].中国兽医杂志, 2021, 57(9):86-90. GONG R C, TIAN X D, OU X N, et al.Effects of phellinus linteus ploysaccharide on growth performance, hematological parameters and immunological indexes of growing-laying hens[J].Chinese Journal of Veterinary Medicine, 2021, 57(9):86-90.(in Chinese)
[22] 吕毅, 李茜茜, 王鑫鑫, 等.日粮添加蛋白酶对肉仔鸡生产性能、氮代谢及粪便氨气释放的影响[J].中国畜牧杂志, 2022, 58(3):123-127, 132. LV Y, LI Q Q, WANG X X, et al.Effects of dietary protease supplementation on performance, nitrogen metabolism and fecal ammonia release of broilers[J].Chinese Journal of Animal Science, 2022, 58(3):123-127, 132.(in Chinese)
[23] 张建, 杨丽娜, 甘悦宁, 等.低蛋白日粮添加蛋氨酸对肉鸡生产性能、氮代谢及血液生化指标的影响[J].饲料工业, 2014(增刊1):55-58. ZHANG J, YANG L N, GAN Y N, et al.Effect of low protein diets supplemented with met on growth performance, nitrogen metabolism and blood biochemical parameters[J].Feed Industry, 2014(Suppl.1):55-58.(in Chinese)
[24] LIU H Y, CAO X X, WANG H, et al.Antimicrobial peptide KR-32 alleviates Escherichia coli K88-induced fatty acid malabsorption by improving expression of fatty acid transporter protein 4(FATP4)[J].Journal of Animal Science, 2019, 97(6):2342-2356.  
[25] CASPARY W F.Physiology and pathophysiology of intestinal absorption[J].The American Journal of Clinical Nutrition, 1992, 55(Suppl.1):299S-308S.
[26] 李朋帅, 乔梦泽, 张雪, 等.万寿菊提取物对太行鸡抗氧化能力及肠道形态的影响[J/OL].中国畜牧杂志:1-9(2022-01-29)[2022-04-16].https://kns.cnki.net/kcms/detail/detail.aspx?FileName=ZGXM20220128002&DbName=CAPJ2022. LI P S, QIAO M Z, ZHANG X, et al.Effects of marigold extracts on antioxidant capacity and intestinal morphology of Taihang chickens[J].Chinese Journal of Animal Science:1-9(2022-01-29)[2022-04-16].https://kns.cnki.net/kcms/detail/detail.aspx?FileName=ZGXM20220128002&DbName=CAPJ2022.(in Chinese)
[27] 沈一茹, 钟光, 胡艳, 等.不同添加方式的酸化剂对肉鸡生长性能和肠道发育的影响[J/OL].动物营养学报:1-10(2022-02-26)[2022-04-16].http://kns.cnki.net/kcms/detail/11.5461.S.20220223.1345.101.html. SHEN Y R, ZHONG G, HU Y, et al.Effects of different supplemental types of acidifier on growth performance and intestinal development of broilers[J/OL].Chinese Journal of Animal Nutrition:1-10(2022-02-26)[2022-04-16].http://kns.cnki.net/kcms/detail/11.5461.S.20220223.1345.101.html.(in Chinese)
[28] 张耀文, 马文峰, 张志丹, 等.天蚕素抗菌肽对产蛋后期蛋鸡生产性能、蛋品质及肠道黏膜形态的影响[J].家畜生态学报, 2020, 41(5):30-35. ZHANG Y W, MA W F, ZHANG Z D, et al.Effects of antimicrobial peptides on laying performance, egg quality and intestinal morphology of hens during the late laying period[J].Journal of Domestic Animal Ecology, 2020, 41(5):30-35.(in Chinese)
[29] 宇晓军, 李丹丹, 厉学武, 等.抗菌肽对育成期母貂免疫功能及肠组织形态的影响[J].中国畜牧兽医, 2022, 49(2):443-453. YU X J, LI D D, LI X W, et al.Effects of antibacterial peptide on the immune function and intestinal tissue morphology of growing female minks[J].China Animal Husbandry & Veterinary Medicine, 2022, 49(2):443-453.(in Chinese)
[30] 徐生良, 刘莉君, 李奎, 等.饲用除臭剂组合对肉鸡生产性能、机体氮代谢和舍内氨气浓度的影响[J].中国家禽, 2021, 43(11):62-67. XU S L, LIU L J, LI K, et al.Effects of feed deodorant combination on growth performance, nitrogen metabolism and concentration of indoor NH3 in broilers[J].China Poultry, 2021, 43(11):62-67.(in Chinese)
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