研究论文 RESEARCH PAPER

罗伊氏乳杆菌肽聚糖对蛋雏鸡生长性能、免疫功能和抗氧化能力的影响

  • 陈晓宇 ,
  • 朱连勤 ,
  • 陈甫 ,
  • 张贝贝 ,
  • 朱风华
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  • 1. 青岛农业大学动物医学学院, 青岛 266109;
    2. 青岛农业大学动物科技学院, 青岛 266109
陈晓宇(1995—),男,山东莒县人,硕士研究生,从事动物营养代谢病与中毒病研究。E-mail:284621446@qq.com

收稿日期: 2022-03-13

  网络出版日期: 2022-10-17

基金资助

山东省现代农业产业技术体系家禽产业创新团队(SDAIT-11-07)

Effects of Lactobacillus reuteri Peptidoglycan on Growth Performance, Immune Function and Antioxidant Capacity of Egg Chicks

  • CHEN Xiaoyu ,
  • ZHU Lianqin ,
  • CHEN Fu ,
  • ZHANG Beibei ,
  • ZHU Fenghua
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  • 1. Department of Animal Medicine, Qingdao Agricultural University, Qingdao 266109, China;
    2. Department of Animal Science, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2022-03-13

  Online published: 2022-10-17

摘要

本试验旨在研究饲粮中添加罗伊氏乳杆菌肽聚糖对蛋雏鸡生长性能、免疫功能和抗氧化能力的影响。试验选取1日龄健康的海兰褐蛋雏鸡96只,随机分为4个组,每组4个重复,每个重复6只。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加250、500、1 000 mg/kg罗伊氏乳杆菌肽聚糖。试验期42 d。结果表明:1)试验第21、42天,1 000 mg/kg罗伊氏乳杆菌肽聚糖组平均日增重显著高于其他各组(P<0.05),1 000 mg/kg罗伊氏乳杆菌肽聚糖组料重比显著低于对照组(P<0.05)。2)试验第42天,500、1 000 mg/kg罗伊氏乳杆菌肽聚糖组血液白细胞总数显著高于对照组和250 mg/kg罗伊氏乳杆菌肽聚糖组(P<0.05)。3)试验第21、42天,1 000 mg/kg罗伊氏乳杆菌肽聚糖组血浆谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性显著高于对照组(P<0.05),500、1 000 mg/kg罗伊氏乳杆菌肽聚糖组血浆总抗氧化能力(T-AOC)显著高于对照组和250 mg/kg罗伊氏乳杆菌肽聚糖组(P<0.05)。4)试验第42天,500、1 000 mg/kg罗伊氏乳杆菌肽聚糖组血浆白细胞介素-2(IL-2)含量显著高于对照组(P<0.05),1 000 mg/kg罗伊氏乳杆菌肽聚糖组血浆免疫球白蛋白A (IgA)、免疫球白蛋白G (IgG)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)和干扰素-γ(IFN-γ)含量显著高于对照组及250、500 mg/kg罗伊氏乳杆菌肽聚糖组(P<0.05)。5)1 000 mg/kg罗伊氏乳杆菌肽聚糖组血浆新城疫病毒抗体(NDV-Ab)和传染性法氏囊病病毒抗体(IBDV-Ab)水平显著高于对照组及250、500 mg/kg罗伊氏乳杆菌肽聚糖组(P<0.05)。6)1 000 mg/kg罗伊氏乳杆菌肽聚糖组脾脏指数显著高于对照组及250、500 mg/kg罗伊氏乳杆菌肽聚糖组(P<0.05),250、1 000 mg/kg罗伊氏乳杆菌肽聚糖组胸腺指数显著高于对照组和500 mg/kg罗伊氏乳杆菌肽聚糖组(P<0.05)。综上所述,饲粮中添加罗伊氏乳杆菌肽聚糖可以提高蛋雏鸡生长性能,促进机体免疫器官的发育,提高机体免疫功能和抗氧化能力。在蛋雏鸡饲粮添加1 000 mg/kg罗伊氏乳杆菌肽聚糖是可行的。

本文引用格式

陈晓宇 , 朱连勤 , 陈甫 , 张贝贝 , 朱风华 . 罗伊氏乳杆菌肽聚糖对蛋雏鸡生长性能、免疫功能和抗氧化能力的影响[J]. 动物营养学报, 2022 , 34(9) : 5741 -5750 . DOI: 10.3969/j.issn.1006-267x.2022.09.029

Abstract

The purpose of this experiment was to study the effects of dietary Lactobacillus reuteri peptidoglycan on growth performance, immune function and antioxidant capacity of egg chicks. Ninety-six healthy 1-day-old Hy-Line Brown egg chicks were randomly divided into 4 groups with 4 replicates in each group and 6 chicks in each replicate. Chicks in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented 250, 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan, respectively. The experiment lasted for 42 days. The results showed as follows:1) on days 21 and 42 of the experiment, the average daily gain of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group was significantly higher than that of other groups (P<0.05), and the feed to gain ratio of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group was significantly lower than that of the control group (P<0.05). 2) On day 42 of the experiment, the white blood cell count of 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of control group and 250 mg/kg Lactobacillus reuteri peptidoglycan group (P<0.05). 3) On days 21 and 42 of the experiment, the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in plasma of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group were significantly higher than those of the control group (P<0.05), and the plasma total antioxidant capacity (T-AOC) of 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of control group and 250 mg/kg Lactobacillus reuteri peptidoglycan group (P<0.05). 4) On day 42 of the experiment, the plasma interleukin-2 (IL-2) content of 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of the control group (P<0.05), and the contents of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-4 (IL-4), interleukin-6 (IL-6) and interferon-γ (IFN-γ) in plasma of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group were significantly higher than those of control group and 250, 500 mg/kg Lactobacillus reuteri peptidoglycan groups (P<0.05). 5) The levels of Newcastle disease virus antibody (NDV-Ab) and infectious bursal disease virus antibody (IBDV-Ab) in plasma of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group were significantly higher than those of control group and 250, 500 mg/kg Lactobacillus reuteri peptidoglycan groups (P<0.05). 6) The spleen index of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group was significantly higher than that of control group and 250, 500 mg/kg Lactobacillus reuteri peptidoglycan groups (P<0.05), and the thymus index of 250 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of control group and 500 mg/kg Lactobacillus reuteri peptidoglycan group (P<0.05). In conclusion, dietary Lactobacillus reuteri peptidoglycan can improve the growth performance of egg chicks, promote the development of immune organs, and improve the body immune function and antioxidant capacity. It is feasible to add 1 000 mg/kg Lactobacillus reuteri peptidoglycan in the diet of egg chicks.

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