动物营养学报 >

2022 , Vol. 34 >Issue 9: 6072 - 6087

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.09.060

研究论文 RESEARCH PAPER

葡萄糖和泌乳相关激素组合添加对奶牛乳腺上皮细胞酪蛋白合成的影响

  • 母晓佳 ,
  • 李大彪 ,
  • 孙梅 ,
  • 曹越 ,
  • 郝怡泓 ,
  • 杨静
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  • 内蒙古农业大学动物科学学院, 动物营养与饲料科学自治区高等学校重点实验室, 呼和浩特 010018
母晓佳(1996—),女,内蒙古苏尼特右旗人,硕士研究生,研究方向为反刍动物营养生理与瘤胃微生态。E-mail:xiaojiamu@163.com

收稿日期: 2022-03-06

  网络出版日期: 2022-10-17

基金资助

国家自然科学基金项目（31860652）

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Effects of Combination of Glucose and Lactation Related Hormones on Casein Synthesis in Bovine Mammary Epithelial Cells

  • MU Xiaojia ,
  • LI Dabiao ,
  • SUN Mei ,
  • CAO Yue ,
  • HAO Yihong ,
  • YANG Jing
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  • Key Laboratory of Animal Nutrition and Feed Science in Universities of Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2022-03-06

  Online published: 2022-10-17

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摘要

本研究以体外培养的奶牛乳腺上皮细胞（BMECs）为模型，探讨葡萄糖和泌乳相关激素组合添加对BMECs酪蛋白合成的影响及作用机制。试验共分为9组，分别向培养基中添加不同浓度的葡萄糖、雌激素和催乳素，其中Ⅰ组为对照组，不添加葡萄糖和泌乳相关激素，Ⅱ组添加14.0 mmol/L葡萄糖+200 ng/mL雌激素，Ⅲ组添加17.5 mmol/L葡萄糖+100 ng/mL雌激素，Ⅳ组添加14.0 mmol/L葡萄糖+100 ng/mL催乳素，Ⅴ组添加17.5 mmol/L葡萄糖+200 ng/mL催乳素，Ⅵ组添加100 ng/mL雌激素+100 ng/mL催乳素，Ⅶ组添加200 ng/mL雌激素+200 ng/mL催乳素，Ⅷ组添加14.0 mmol/L葡萄糖+100 ng/mL雌激素+200 ng/mL催乳素，Ⅸ组添加17.5 mmol/L葡萄糖+200 ng/mL雌激素+100 ng/mL催乳素，每组设置6个重复。采用四甲基偶氮唑盐（MTT）法检测细胞增殖率；采用实时荧光定量PCR （RT-qPCR）法检测葡萄糖转运载体、泌乳相关激素受体、酪蛋白合成相关基因、酪氨酸激酶2/信号转导及转录激活因子5（JAK2/STAT5）信号通路及单磷酸腺苷活化蛋白激酶/哺乳动物雷帕霉素靶蛋白（AMPK/mTOR）信号通路相关基因表达；采用蛋白质印迹法（Western blot）检测细胞中α-酪蛋白、mTOR、核糖体p70s6激酶（S6K）和AMPK蛋白表达及其磷酸化水平。结果表明：1）与对照组相比，各试验组BMECs的增殖率均显著提高（P<0.05），Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅷ和Ⅸ组BMECs增殖率显著高于Ⅵ和Ⅶ组（P<0.05），且Ⅷ组的效果最佳。2）Ⅷ组BMECs激素受体基因[雌激素α受体（ER-α）、雌激素β受体（ER-β）、G蛋白偶联受体30（GPER30）和催乳素受体（PRLR）]，mTOR信号通路相关基因[mTOR、蛋白激酶B （AKT）、磷脂酰肌醇-3-羟激酶（PI3K）和真核翻译启始因子4E （eIF4E）]，JAK2/STAT5信号通路相关基因（JAK2和STAT5）及κ-酪蛋白（CSN3）基因的相对表达量最高，且mTOR和S6K蛋白磷酸化水平最高，对BMECs酪蛋白合成的促进效果最佳。3）Ⅸ组BMECs的αs1-酪蛋白（CSN1S1）、β-酪蛋白（CSN2）和核糖体p70s6（S6K1）基因相对表达量最高。4）Ⅴ、Ⅷ和Ⅸ组的mTOR磷酸化水平和酪蛋白表达量显著高于其他试验组（P<0.05）。综上所述，组合添加14.0 mmol/L葡萄糖+100 ng/mL雌激素+200 ng/mL催乳素对BMECs酪蛋白合成的促进效果最佳。

关键词： 奶牛乳腺上皮细胞; 葡萄糖; 激素; 酪蛋白

本文引用格式

母晓佳 , 李大彪 , 孙梅 , 曹越 , 郝怡泓 , 杨静 . 葡萄糖和泌乳相关激素组合添加对奶牛乳腺上皮细胞酪蛋白合成的影响[J]. 动物营养学报, 2022 , 34(9) : 6072 -6087 . DOI: 10.3969/j.issn.1006-267x.2022.09.060

Abstract

This experiment was to investigate the effects of combined addition of glucose and lactation-related hormone on casein synthesis in bovine mammary epithelial cells (BMECs) and mechanism of action by using BMECs in vitro as a model. The experiment was divided into nine groups, and different concentrations of glucose, estrogen and prolactin were added to the culture medium, group Ⅰ was the control group without glucose and lactation hormones, group Ⅱ was added with 14.0 mmol/L glucose+200 ng/mL estrogen, group Ⅲ was added with 17.5 mmol/L glucose +100 ng/mL estrogen, group Ⅳ was added with 14.0 mmol/L glucose+100 ng/mL prolactin, group Ⅴ was added with 17.5 mmol/L glucose+200 ng/mL prolactin, group Ⅵ was added with 100 ng/mL estrogen+100 ng/mL prolactin, group Ⅶ was added with 200 ng/mL estrogen+200 ng/mL prolactin, group Ⅷ was added with 14.0 mmol/L glucose+100 ng/mL estrogen+200 ng/mL prolactin, group Ⅸ was added with 17.5 mmol/L glucose+200 ng/mL estrogen+100 ng/mL prolactin, and each group was set with 6 replicates. Cell proliferation rate was detected by MTT method; real-time fluorescence quantitative PCR (RT-qPCR) method was used to detect the expression of glucose transporter carrier, lactation-related hormone receptor, casein synthesis-related genes, tyrosine kinase 2/signal transduction and transcription factor 5 (JAK2/STAT5) signaling pathway and adenosine monophosphate activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signaling pathway-related genes; protein blotting (Western blot) was used to detect the expression of α-casein, mTOR, ribosomal p70s6 kinase (S6K) and AMPK proteins and their phosphorylation levels. The results showed as follows:1) compared with the control group, the proliferation rate of BMECs was significantly increased in all the test groups (P<0.05), and the proliferation rate of BMECs in the groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅷ and Ⅸ was significantly higher than that in the groups Ⅵ and Ⅶ (P<0.05), and the best results were obtained in the group Ⅷ. 2) The relative expression levels of hormone receptor genes[estrogen α receptor (ER-α), estrogen β receptor (ER-β), G protein-coupled receptor 30 (GPER30) and prolactin receptor (PRLR)], mTOR signaling pathway related genes[mTOR, protein kinase B (AKT), phosphatidylinositol-3-hydroxykinase (PI3K) and eukaryotic translation initiation factor 4E (eIF4E)] and JAK2/STAT5 signaling pathway related genes (JAK2 and STAT5) and к-casein (CSN3) gens in group Ⅷ were the highest, and the phosphorylation levels of mTOR and S6K were the highest, which showed the best promoting effect on BMECs casein synthesis. 3) The relative expression levels of α S1-casein (CSN1S1), β-casein (CSN2) and ribosomal p70s6 (S6K1) genes of BMECs in group Ⅸ were the highest. 4) The phosphorylation level and casein expression of mTOR in groups Ⅴ, Ⅷ and Ⅸ were significantly higher than those in the other test groups (P<0.05). Taken together, the combination addition of glucose 14.0 mmol/L, estrogen 100 ng/mL and prolactin 200 ng/mL has the best effect on the promotion of casein synthesis in BMECs; the combination addition of glucose and prolactin has the greatest effect on casein synthesis.

Key words： cow mammary epithelial cells; glucose; hormone; casein

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