饲料营养研究进展专栏 SPECIAL COLUMN:RESEARCH PROGRESS ON FEED NUTRITION

肠道类器官在畜禽营养中的研究进展

  • 王丹 ,
  • 刘玉兰
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  • 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉 430023
王丹(1991-),男,湖北石首人,讲师,博士,从事动物营养研究。E-mail:wangdan@whpu.edu.cn

收稿日期: 2022-08-01

  网络出版日期: 2022-10-17

基金资助

国家自然科学基金项目(32102566)

Research Progress of Intestinal Organoids in Livestock and Poultry Nutrition

  • WANG Dan ,
  • LIU Yulan
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2022-08-01

  Online published: 2022-10-17

摘要

肠道类器官是由单个肠道干细胞发育而来的具有隐窝-绒毛结构及所有种类的肠道上皮细胞的结构,也称为"迷你肠"。由于其与肠道结构和生理环境具有高度的一致性,肠道类器官是代替肠道组织的良好模型。目前,肠道类器官已经应用于畜禽动物的营养吸收评价、肠道微生物与宿主互作等方面的研究。本文综述了肠道类器官的研究进展以及关键信号通路在其中的作用,并探讨了肠道类器官在畜禽营养研究中的应用前景。

本文引用格式

王丹 , 刘玉兰 . 肠道类器官在畜禽营养中的研究进展[J]. 动物营养学报, 2022 , 34(10) : 6352 -6357 . DOI: 10.3969/j.issn.1006-267x.2022.10.028

Abstract

Intestinal organoids, also known as "mini-gut", contain crypt-villi structures and all types of intestinal epithelial cell structures, which are derived from a single intestinal stem cell. Due to their high consistency with intestinal structure and physiological environment, intestinal organoids are a good model to replace intestinal tissue. At present, intestinal organoids have been used in the evaluation of nutrient absorption and the interaction between intestinal microbes and host in livestock and poultry. This review summarized the recent research progress of intestinal organoids and their key signaling pathways, as well as their potential application in livestock and poultry nutrition.

参考文献

[1] BARKER N,VAN ES J H,KUIPERS J,et al.Identification of stem cells in small intestine and colon by marker gene Lgr5[J].Nature,2007,449(7165):1003-1007.  
[2] DERRICOTT H,LUU L,FONG W Y,et al.Developing a 3D intestinal epithelium model for livestock species[J].Cell and Tissue Research,2019,375(2):409-424.  
[3] HAMILTON C A,YOUNG R,JAYARAMAN S,et al.Development of in vitro enteroids derived from bovine small intestinal crypts[J].Veterinary Research,2018,49(1):54.
[4] KHALIL H A,LEI N Y,BRINKLEY G,et al.A novel culture system for adult porcine intestinal crypts[J].Cell and Tissue Research,2016,365(1):123-134.  
[5] GONZALEZ L M,WILLIAMSON I,PIEDRAHITA J A,et al.Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration[J].PLoS One,2013,8(6):e66465.
[6] PIERZCHALSKA M,GRABACKA M,MICHALIK M,et al.Prostaglandin E2 supports growth of chicken embryo intestinal organoids in Matrigel matrix[J].BioTechniques,2012,52(5):307-315.  
[7] BARKER N.Adult intestinal stem cells:critical drivers of epithelial homeostasis and regeneration[J].Nature Reviews Molecular Cell Biology,2014,15:19-33.
[8] TIAN H,BIEHS B,WARMING S,et al.A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable[J].Nature,2011,478(7368):255-259.  
[9] SATO T,VRIES R G,SNIPPERT H J,et al.Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche[J].Nature,2009,459(7244):262-265.  
[10] MERENDA A,FENDERICO N,MAURICE M M.Wnt signaling in 3D:recent advances in the applications of intestinal organoids[J].Trends in Cell Biology,2020,30(1):60-73.  
[11] GEHART H,CLEVERS H.Tales from the crypt:new insights into intestinal stem cells[J].Nature Reviews Gastroenterology&Hepatology,2019,16(1):19-34.  
[12] CARMON K S,LIN Q S,GONG X,et al.LGR5 interacts and cointernalizes with Wnt receptors to modulate Wnt/β-catenin signaling[J].Molecular and Cellular Biology,2012,32(11):2054-2064.  
[13] TAKAHASHI T,SHIRAISHI A.Stem cell signaling pathways in the small intestine[J].International Journal of Molecular Sciences,2020,21(6):2032.
[14] HARAMIS A P G,BEGTHEL H,VAN DEN BORN M,et al.De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine[J].Science,2004,303(5664):1684-1686.  
[15] HE X C,ZHANG J W,TONG W G,et al.BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-β-catenin signaling[J].Nature Genetics,2004,36(10):1117-1121.  
[16] KOSINSKI C,LI V S W,CHAN A S Y,et al.Gene expression patterns of human colon tops and basal crypts and BMP antagonists as intestinal stem cell niche factors[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(39):15418-15423.  
[17] LUDIKHUIZE M C,MEERLO M,GALLEGO M P,et al.Mitochondria define intestinal stem cell differentiation downstream of a FOXO/notch axis[J].Cell Metabolism,2020,32(5):889-900.e7.  
[18] LIANG S J,LI X G,WANG X Q.Notch signaling in mammalian intestinal stem cells:determining cell fate and maintaining homeostasis[J].Current Stem Cell Research&Therapy,2019,14(7):583-590.  
[19] SNIPPERT H J,VAN DER FLIER L G,SATO T,et al.Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells[J].Cell,2010,143(1):134-144.  
[20] SHROYER N F,HELMRATH M A,WANG V Y C,et al.Intestine-specific ablation of mouse atonal homolog 1(Math1) reveals a role in cellular homeostasis[J].Gastroenterology,2007,132(7):2478-2488.  
[21] VANDUSSEN K L,SAMUELSON L C.Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate[J].Developmental Biology,2010,346(2):215-223.  
[22] MO J S,PARK H W,GUAN K L.The hippo signaling pathway in stem cell biology and cancer[J].EMBO Reports,2014,15(6):642-656.  
[23] BARRY E R,MORIKAWA T,BUTLER B L,et al.Restriction of intestinal stem cell expansion and the regenerative response by YAP[J].Nature,2013,493(7430):106-110.  
[24] GREGORIEFF A,LIU Y,INANLOU M R,et al.Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer[J].Nature,2015,526(7575):715-718.  
[25] PANEK M,GRABACKA M,PIERZCHALSKA M.The formation of intestinal organoids in a hanging drop culture[J].Cytotechnology,2018,70(3):1085-1095.  
[26] MUSSARD E,POUZET C,HELIES V,et al.Culture of rabbit caecum organoids by reconstituting the intestinal stem cell niche in vitro with pharmacological inhibitors or L-WRN conditioned medium[J].Stem Cell Research,2020,48:101980.
[27] VAN DER HEE B,LOONEN L M P,TAVERNE N,et al.Optimized procedures for generating an enhanced,near physiological 2D culture system from porcine intestinal organoids[J].Stem Cell Research,2018,28:165-171.
[28] LI L,FU F,GUO S S,et al.Porcine intestinal enteroids:a new model for studying enteric coronavirus porcine epidemic diarrhea virus infection and the host innate response[J].Journal of Virology,2019,93(5):e01682-18.
[29] ENGEVIK A C,COUTTS A W,KAJI I,et al.Editing myosin VB gene to create porcine model of microvillus inclusion disease,with microvillus-lined inclusions and alterations in sodium transporters[J].Gastroenterology,2020,158(8):2236-2249.e9.  
[30] LI J,LI J J,ZHANG S Y,et al.Culture and characterization of chicken small intestinal crypts[J].Poultry Science,2018,97(5):1536-1543.  
[31] VAN DER HEE B,MADSEN O,VERVOORT J,et al.Congruence of transcription programs in adult stem cell-derived jejunum organoids and original tissue during long-term culture[J].Frontiers in Cell and Developmental Biology,2020,8:375.
[32] HOU Q H,HUANG J X,XIONG X,et al.Role of nutrient-sensing receptor GPRC6A in regulating colonic group 3 innate lymphoid cells and inflamed mucosal healing[J].Journal of Crohn's&Colitis,2022:jjac020.
[33] ZHU M,QIN Y C,GAO C Q,et al.L-glutamate drives porcine intestinal epithelial renewal by increasing stem cell activity via upregulation of the EGFR-ERK-mTORC1 pathway[J].Food&Function,2020,11(3):2714-2724.  
[34] WANG Z B,LI J,WANG Y,et al.Dietary vitamin A affects growth performance,intestinal development,and functions in weaned piglets by affecting intestinal stem cells[J].Journal of Animal Science,2020,98(2):skaa020.
[35] YIN L D,CHEN J F,LI L,et al.Aminopeptidase N expression,not interferon responses,determines the intestinal segmental tropism of porcine deltacoronavirus[J].Journal of Virology,2020,94(14):e00480-20.
[36] LI Y,YANG N,CHEN J N,et al.Next-generation porcine intestinal organoids:an apical-out organoid model for swine enteric virus infection and immune response investigations[J].Journal of Virology,2020,94(21):e01006-e01020.
[37] LUO H,ZHENG J Y,CHEN Y L,et al.Utility evaluation of porcine enteroids as PDCoV infection model in vitro[J].Frontiers in Microbiology,2020,11:821.
[38] ALFAJARO M M,KIM J Y,BARB L,et al.Dual recognition of sialic acid and αGal epitopes by the VP8* domains of the bovine rotavirus G6P
[5] WC3 and of its mono-reassortant G4P
[5] RotaTeq vaccine strains[J].Journal of Virology,2019,93(18):e00941-19.
[39] HOU Q H,YE L L,LIU H F,et al.Lactobacillus accelerates ISCs regeneration to protect the integrity of intestinal mucosa through activation of STAT3 signaling pathway induced by LPLs secretion of IL-22[J].Cell Death and Differentiation,2018,25(9):1657-1670.  
[40] WILLIAMSON I A,ARNOLD J W,SAMSA L A,et al.A high-throughput organoid microinjection platform to study gastrointestinal microbiota and luminal physiology[J].Cellular and Molecular Gastroenterology and Hepatology,2018,6(3):301-319.  
[41] PIERZCHALSKA M,PANEK M,CZYRNEK M,et al.Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[J].Comparative Immunology, Microbiology and Infectious Diseases,2017,53:7-18.
[42] BEAUMONT M,PAËS C,MUSSARD E,et al.Gut microbiota derived metabolites contribute to intestinal barrier maturation at the suckling-to-weaning transition[J].Gut Microbes,2020,11(5):1268-1286.  
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