研究论文 RESEARCH PAPER

不同蛋白质来源的饲料原料饲喂生长猪有效能值和氨基酸消化率评定

  • 麻龙腾 ,
  • 吴聪 ,
  • 王丽芬 ,
  • 张梓熹 ,
  • 宋泽和 ,
  • 贺喜 ,
  • 马晓康
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  • 湖南农业大学动物科学技术学院, 长沙 410128
麻龙腾(1993-),男,湖南湘西人,硕士研究生,从事动物营养与饲料科学的研究。E-mail:malongteng2021@163.com

收稿日期: 2022-02-23

  网络出版日期: 2022-10-17

基金资助

财政部和农业农村部国家现代农业产业技术体系——国家生猪产业技术体系建设专项(CARS-35)

Evaluation of Effective Energy Value and Amino Acid Digestibility of Feed Materials with Different Protein Sources of Growing Pigs

  • MA Longteng ,
  • WU Cong ,
  • WANG Lifen ,
  • ZHANG Zixi ,
  • SONG Zehe ,
  • HE Xi ,
  • MA Xiaokang
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  • College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2022-02-23

  Online published: 2022-10-17

摘要

试验旨在测定浓缩脱酚棉籽蛋白(CDCP)、2种酶解豆粕[50%酸溶蛋白(SP-50)、20%酸溶蛋白(SP-20)]和肠膜蛋白(DPS)饲喂生长猪消化能、代谢能和氨基酸回肠末端消化率。试验1:选用36头初始体重为(26.67±1.48) kg的"杜×长×大"三元杂交去势公猪,随机分成6组,每组6个重复,每个重复1头猪。各组分别饲喂玉米基础饲粮以及CDCP、2种酶解豆粕、DPS和鱼粉(FM)饲粮,采用全收粪法测定消化能和代谢能。试验2:选用12头初始体重为(15.5±0.45) kg安装T型瘘管的"杜×长×大"三元杂交去势公猪,随机分成6组,每组2个重复,每个重复1头猪。各组分别饲喂无氮饲粮以及CDCP、2种酶解豆粕、DPS和FM饲粮,采用指示剂法计算氨基酸的表观回肠末端消化率(AID)和标准回肠末端消化率(SID)。结果表明: CDCP和SP-50的总能和粗蛋白质含量高于DPS; SP-20的总能高于DPS,低于FM,粗蛋白质含量低于DPS和FM。SP-50的总氨基酸AID显著高于CDCP、SP-20、DPS和FM(P<0.05), CDCP、SP-20和FM的总氨基酸AID显著高于DPS(P<0.05)。CDCP、SP-50、SP-20和FM的总氨基酸SID显著高于DPS(P<0.05)。由此可见, 2种酶解豆粕(SP-50、SP-20)和CDCP的氨基酸AID和SID与FM相近。单就氨基酸的SID而言, SP-50、SP-20和CDCP可以作为潜在的蛋白质原料替代品应用于生长猪饲粮。

本文引用格式

麻龙腾 , 吴聪 , 王丽芬 , 张梓熹 , 宋泽和 , 贺喜 , 马晓康 . 不同蛋白质来源的饲料原料饲喂生长猪有效能值和氨基酸消化率评定[J]. 动物营养学报, 2022 , 34(10) : 6469 -6482 . DOI: 10.3969/j.issn.1006-267x.2022.10.042

Abstract

This experiment was conducted to evaluate the digestible energy, metabolizable energy and amino acid terminal ileum digestibility of concentrated dephenolized cottonseed protein (CDCP), two enzymolized soybean meals [50% acid soluble protein (SP-50), 20% acid soluble protein (SP-20)] and intestine membrane protein meal (DPS) of growing pigs.) In experiment 1, thirty-six crossbred castrated boars (Duroc×Landraces×Yorkshire) with an initial body weight of (26.67±1.48) kg were randomly divided into 6 groups with 6 replicates per group and 1 pig per replicate. Pigs in 6 groups were fed the corn basal diet and CDCP, two enzymolized soybean meals, eDPS and fish meal (FM) diets, respectively, and the digestible energy and metabolizable energy were determined by total fecal harvesting method. In experiment 2, twelve crossbred castrated boars (Duroc×Landraces×Yorkshire) with an initial body weight of (15.5±0.45) kg installed with T-shaped cannulas were randomly divided into 6 groups with 2 replicates per group and 1 pig per replicate. Pigs in 6 groups were fed the nitrogen-free diet and CDCP, two enzymolized soybean meals, DPS and FM diets, respectively, and the apparent terminal ileal digestibility (AID) and standard terminal ileal digestibility (SID) of amino acids were calculated by indicator method. The results showed that the gross energy and crude protein content of CDCP and SP-50 were higher than those of DPS; the gross energy of SP-20 was higher than that of DPS and lower than that of FM, and the crude protein content was lower than that of DPS and FM. The AID of total amino acids of SP-50 was significantly higher than that of CDCP, SP-20, DPS and FM (P < 0.05), and the AID of total amino acids of CDCP, SP-20 and FM was significantly higher than that of DPS (P < 0.05). The SID of total amino acids of CDCP, SP-50, SP-20 and FM was significantly higher than that of DPS (P < 0.05). In conclusion, the AID and SID of amino acids of two enzymolized soybean meals (SP-50 and SP-20) and CDCP were similar to FM. In terms of SID of amino acids, SP-50, SP-20 and CDCP can be used as potential Protein raw material substitutes in growing pig diets.

参考文献

[1] CHIBA L I.Protein supplements[M]//LEWIS A J,SOUTHERN L L.Swine nutrition.2nd ed.Boca Raton:CRC Press,2000:804-839.
[2] ZHANG S,PIAO X S,MA X K,et al.Comparison of spray-dried egg and albumen powder with conventional animal protein sources as feed ingredients in diets fed to weaned pigs[J].Animal Science Journal,2015,86(8):772-781.  
[3] KIM S W,EASTER R A.Nutritional value of fish meals in the diet for young pigs[J].Journal of Animal Science,2001,79(7):1829-1839.  
[4] JEONG J S,PARK J W,LEE S I,et al.Apparent ileal digestibility of nutrients and amino acids in soybean meal,fish meal,spray-dried plasma protein and fermented soybean meal to weaned pigs[J].Animal Science Journal,2016,87(5):697-702.  
[5] BERGSTRÖM J R,NELSSEN J L,TOKACH M D,et al.Evaluation of spray-dried animal plasma and select menhaden fish meal in transition diets of pigs weaned at 12 to 14 days of age and reared in different production systems[J].Journal of Animal Science,1997,75(11):3004-3009.  
[6] MYERS A J,GOODBAND R D,TOKACH M D,et al.The effects of porcine intestinal mucosa protein sources on nursery pig growth performance[J].Journal of Animal Science,2014,92(2):783-792.  
[7] SULABO R C,MATHAI J K,USRY J L,et al.Nutritional value of dried fermentation biomass,hydrolyzed porcine intestinal mucosa products,and fish meal fed to weanling pigs[J].Journal of Animal Science,2013,91(6):2802-2811.  
[8] SULABO R C,JU W S,STEIN H H.Amino acid digestibility and concentration of digestible and metabolizable energy in copra meal,palm kernel expellers,and palm kernel meal fed to growing pigs[J].Journal of Animal Science,2013,91(3):1391-1399.  
[9] CERVANTES-PAHM S K,STEIN H H.Ileal digestibility of amino acids in conventional,fermented,and enzyme-treated soybean meal and in soy protein isolate,fish meal,and casein fed to weanling pigs[J].Journal of Animal Science,2010,88(8):2674-2683.  
[10] COTTEN B,RAGLAND D,THOMSON J E,et al.Amino acid digestibility of plant protein feed ingredients for growing pigs[J].Journal of Animal Science,2016,94(3):1073-1082.  
[11] 李忠超.生长猪植物蛋白原料净能推测方程的构建[D].博士学位论文.北京:中国农业大学,2017. LI Z C.Net energy prediction of plant protein ingredients to growing pigs[D].Ph.D.Thesis.Beijing:China Agricultural University,2017.(in Chinese)
[12] MA X K,ZHANG S,SHANG Q H,et al.Determination and prediction of the apparent and standardized ileal amino acid digestibility in cottonseed meals fed to growing pigs[J].Animal Science Journal,2019,90(5):655-666.  
[13] HULSHOF T G,VAN DER POEL A F B,HENDRIKS W H,et al.Processing of soybean meal and 00-rapeseed meal reduces protein digestibility and pig growth performance but does not affect nitrogen solubilization along the small intestine[J].Journal of Animal Science,2016,94(6):2403-2414.  
[14] ZENG Q F,YANG G L,LIU G N,et al.Effects of dietary gossypol concentration on growth performance,blood profiles,and hepatic histopathology in meat ducks[J].Poultry Science,2014,93(8):2000-2009.  
[15] JEONG J S,KIM I H.Comparative efficacy of up to 50% partial fish meal replacement with fermented soybean meal or enzymatically prepared soybean meal on growth performance,nutrient digestibility and fecal microflora in weaned pigs[J].Animal Science Journal,2015,86(6):624-633.  
[16] LI R,HOU G F,SONG Z H,et al.Effects of different protein sources completely replacing fish meal in low-protein diet on growth performance,intestinal digestive physiology,and nitrogen digestion and metabolism in nursery pigs[J].Animal Science Journal,2019,90(8):977-989.  
[17] LI R,HOU G F,SONG Z H,et al.Nutritional value of enzyme-treated soybean meal, concentrated degossypolized cottonseed protein,dried porcine solubles and fish meal for 10-to-20 kg pigs[J].Animal Feed Science and Technology,2019,252:23-33.
[18] GOEBEL K P,STEIN H H.Phosphorus digestibility and energy concentration of enzyme-treated and conventional soybean meal fed to weanling pigs[J].Journal of Animal Science,2011,89(3):764-772.  
[19] STEIN H H,SHIPLEY C F,EASTER R A.Technical note:a technique for inserting a T-cannula into the distal ileum of pregnant sows[J].Journal of Animal Science,1998,76(5):1433-1436.  
[20] Committee on Nutrient Requirements of Swine,Board on Agriculture and Natural Resources,Division on Earth and Life Studies,et al.Nutrient requirements of swine[M].11th ed.Washington,D.C.:National Academies Press,2012.
[21] 肖志明,李丽蓓,邓涛,等.饲料原料中酸溶蛋白的测定方法研究[J].中国畜牧杂志,2016,52(2):72-75,80. XIAO Z M,LI L B,DENG T,et al.Study for the determination of acid-soluble protein in feed materials[J].Chinese Journal of Animal Science,2016,52(2):72-75,80.(in Chinese)
[22] 姜友军,付爱华,杨超,等.大豆水溶性蛋白质测定的影响因素探讨[J].粮食储藏,2016,45(6):32-35. JIANG Y J,FU A H,YANG C,et al.Discussion on factors in fluencing the measuring methods of water-soluble protein in soybean[J].Grain Storage,2016,45(6):32-35.(in Chinese)
[23] 刘桂宾,张璐,李元,等.豆粕蛋白溶解度测定方法的研究[J].检验检疫科学,2007,17(1):36-39. LIU G B,ZHANG L,LI Y,et al.Study on the test of the analytical methods of the protein solubility of soybean meals[J].Inspection and Quarantine Science,2007,17(1):36-39.(in Chinese)
[24] 邓雪娟,刘国华,蔡辉益,等.分光光度计法测定家禽饲料和食糜中二氧化钛[J].饲料工业,2008,29(2):57-58. DENG X J,LIU G H,CAI H Y,et al.Determine of titanium dioxide in poultry feed and chyme by spectrophotometer[J].Feed Industry,2008,29(2):57-58.(in Chinese)
[25] 张铖铖,李敏,张石蕊,等.猪脱酚棉籽蛋白能量及粗蛋白质消化率的比较评定[J].饲料工业,2012,33(6):26-28. ZHANG C C,LI M,ZHANG S R,et al.Evaluation of digestible energy content and apparent digestibility of crude protein of degossypolled cottonseed protein (DCP) for swine[J].Feed Industry,2012,33(6):26-28.(in Chinese)
[26] ZHANG W J,XU Z R,ZHAO S H,et al.Development of a microbial fermentation process for detoxification of gossypol in cottonseed meal[J].Animal Feed Science and Technology,2007,135(1/2):176-186.
[27] 王开丽,张石蕊,贺喜,等.脱酚棉籽蛋白对黄羽肉鸡生产性能、屠宰性能和血液指标的影响[J].中国家禽,2013,35(13):23-27. WANG K L,ZHANG S R,HE X,et al.Effects of degossypolized cottonseed protein on production performance,slaughter performance and blood indexes of yellow-feather broiler[J].China Poultry,2013,35(13):23-27.(in Chinese)
[28] 沈俊,陈达图,胡官波,等.生长猪脱酚棉籽蛋白消化能的评定及估测模型研究[J].动物营养学报,2014,26(8):2262-2269. SHEN J,CHEN D T,HU G B,et al.Evaluation and prediction model of digestible energy of degossypolized cottonseed protein for growing pigs[J].Chinese Journal of Animal Nutrition,2014,26(8):2262-2269.(in Chinese)
[29] ROJAS O J,STEIN H H.Concentration of digestible and metabolizable energy and digestibility of amino acids in chicken meal,poultry byproduct meal,hydrolyzed porcine intestines,a spent hen-soybean meal mixture,and conventional soybean meal fed to weanling pigs[J].Journal of Animal Science,2013,91(7):3220-3230.  
[30] YAO K,GUAN S,LI T J,et al.Dietary L-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weanling piglets[J].British Journal of Nutrition,2011,105(5):703-709.  
[31] YAO K,YIN Y L,CHU W Y,et al.Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs[J].The Journal of Nutrition,2008,138(5):867-872.  
[32] MATEOS G G,MOHITI-ASLI M,BORDA E,et al.Effect of inclusion of porcine mucosa hydrolysate in diets varying in lysine content on growth performance and ileal histomorphology of broilers[J].Animal Feed Science and Technology,2014,187:53-60.
[33] FRIKHA M,MOHITI-ASLI M,CHETRIT C,et al.Hydrolyzed porcine mucosa in broiler diets:effects on growth performance,nutrient retention,and histomorphology of the small intestine[J].Poultry Science,2014,93(2):400-411.  
[34] BAKER K M,STEIN H H.Amino acid digestibility and concentration of digestible and metabolizable energy in soybean meal produced from conventional,high-protein,or low-oligosaccharide varieties of soybeans and fed to growing pigs[J].Journal of Animal Science,2009,87(7):2282-2290.  
[35] JACELA J Y,FROBOSE H L,DEROUCHEY J M,et al.Amino acid digestibility and energy concentration of high-protein corn dried distillers grains and high-protein sorghum dried distillers grains with solubles for swine[J].Journal of Animal Science,2010,88(11):3617-3623.  
[36] LIU Y,SONG M,ALMEIDA F N,et al.Energy concentration and amino acid digestibility in corn and corn coproducts from the wet-milling industry fed to growing pigs[J].Journal of Animal Science,2014,92(10):4557-4565.  
[37] KIM B G,LIU Y,STEIN H H.Energy concentration and phosphorus digestibility in yeast products produced from the ethanol industry,and in brewers'yeast,fish meal,and soybean meal fed to growing pigs[J].Journal of Animal Science,2014,92(12):5476-5484.  
[38] SUN H,TANG J W,YAO X H,et al.Effects of dietary inclusion of fermented cottonseed meal on growth,cecal microbial population,small intestinal morphology,and digestive enzyme activity of broilers[J].Tropical Animal Health and Production,2013,45(4):987-993.  
[39] CRANSTON J J,RIVERA J D,GALYEAN M L,et al.Effects of feeding whole cottonseed and cottonseed products on performance and carcass characteristics of finishing beef cattle[J].Journal of Animal Science,2006,84(8):2186-2199.  
[40] STEIN H H,SE VE B,FULLER M F,et al.Invited review:amino acid bioavailability and digestibility in pig feed ingredients:terminology and application[J].Journal of Animal Science,2007,85(1):172-180.  
[41] PANGENI D,JENDZA J A,ANIL L,et al.Effect of replacing conventional soybean meal with low-oligosaccharide soybean meal on growth performance and carcass characteristics of wean-to-finish pigs[J].Journal of Animal Science,2017,95(6):2605-2613.
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