动物营养学报 >

2022 , Vol. 34 >Issue 10: 6491 - 6501

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.10.044

研究论文 RESEARCH PAPER

氧化锌对感染猪流行性腹泻病毒的新生阶段仔猪生长性能、肠道屏障功能和抗氧化能力的影响

  • 王思甜 ,
  • 郑丽云 ,
  • 齐雅 ,
  • 刘志鹏 ,
  • 郭双双 ,
  • 侯永清 ,
  • 张正帆 ,
  • 丁斌鹰
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  • 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
王思甜(1997-),女,辽宁盘锦人,硕士研究生,研究方向动物营养与饲料科学。E-mail:2419454259@qq.com

收稿日期: 2022-04-02

  网络出版日期: 2022-10-17

基金资助

湖北省中央引导地方科技发展专项(2021BGE047)

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Effects of Zinc Oxide on Growth Performance, Intestinal Barrier Function and Antioxidant Capacity of Neonatal Piglets Infected with Porcine Epidemic Diarrhea Virus

  • WANG Sitian ,
  • ZHENG Liyun ,
  • QI Ya ,
  • LIU Zhipeng ,
  • GUO Shuangshuang ,
  • HOU Yongqing ,
  • ZHANG Zhengfan ,
  • DING Binying
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2022-04-02

  Online published: 2022-10-17

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摘要

本试验旨在研究氧化锌(ZnO)对感染猪流行性腹泻病毒(PEDV)的新生阶段仔猪生长性能、肠道屏障功能和抗氧化能力的影响。采用完全随机区组试验设计,将24头平均体重为(2.5±0.2) kg的健康7日龄仔猪(杜×长×大)随机分为4组,分别为对照组、ZnO组、PEDV组和PEDV+ZnO组,每组6个重复,每个重复1头猪。试验期为12 d,分为感染前(第1~8天)和感染后(第9~12天)2个阶段。ZnO组和PEDV+ZnO组每天灌服100 mg/kg BW ZnO;第9天07:00, PEDV组和PEDV+ZnO组每头仔猪灌服1 mL的PEDV溶液(含106 TCID50),对照组和ZnO组灌服等体积的磷酸盐缓冲溶液(PBS)。结果表明:感染前,与对照组相比,灌服ZnO仔猪料重比(F/G)显著降低(P < 0.05)。灌服ZnO显著降低了PEDV感染造成的仔猪的F/G、腹泻指数、空肠肠道评分和血浆二胺氧化酶活性(DAO)的显著增加(P < 0.05);灌服ZnO缓解了PEDV感染造成的仔猪空肠绒毛高度(VH)和绒毛面积(VA)以及空肠隐窝深度(CD)显著下降(P < 0.05);灌服ZnO显著提高了PEDV感染造成的仔猪血清过氧化氢酶(CAT)活性显著降低(P < 0.05);灌服ZnO缓解了PEDV感染造成的仔猪空肠谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性的显著降低与十二指肠丙二醛(MDA)含量的显著升高(P < 0.05)。综上所述,灌服100 mg/kg BW ZnO能通过改善小肠形态,增强肠道屏障功能,提高抗氧化能力,改善PEDV感染仔猪生长性能。

关键词： 氧化锌; PEDV; 生长性能; 肠道屏障功能; 抗氧化功能

本文引用格式

王思甜 , 郑丽云 , 齐雅 , 刘志鹏 , 郭双双 , 侯永清 , 张正帆 , 丁斌鹰 . 氧化锌对感染猪流行性腹泻病毒的新生阶段仔猪生长性能、肠道屏障功能和抗氧化能力的影响[J]. 动物营养学报, 2022 , 34(10) : 6491 -6501 . DOI: 10.3969/j.issn.1006-267x.2022.10.044

Abstract

This study was conducted to investigate the effects of zinc oxide (ZnO) on growth performance, intestinal barrier function and antioxidant capacity of neonatal piglets infected with porcine epidemic diarrhea virus (PEDV). In a completely randomized block design, twenty-four 7-day-old piglets (Duroc×Landrace×Yorkshire), with an average body weight of (2.5±0.2) kg, were randomly divided into 4 groups with 6 replicates per group and 1 pig per replicate. The groups were as follows: control group, ZnO group, PEDV group and PEDV+ZnO group. The experiment lasted for 12 days and was divided into two stages as pre-infected stage (days 1 to 8) and infected stage (days 9 to 12). Piglets in the ZnO group and PEDV+ZnO group were orally administered with 100 mg/kg BW ZnO every day. Piglets in the PEDV group and PEDV+ZnO group were orally inoculated with 1 mL PEDV solution at a dose of 106 TCID50 (50% tissue culture infection dose) per pig on day 9 at 07:00, while piglets in the control group and ZnO group were given equal volume phosphate buffer solution (PBS). The results showed that ZnO administration decreased the feed to gain ratio (F/G) compared with the control group before infection (P < 0.05). The F/G, diarrhea rate, jejunal intestinal score and plasma diamine oxidase activity of piglets were significantly increased by PEDV infection (P < 0.05), while those could be significantly decreased by ZnO administration (P < 0.05). The villus height (VH) and villus area (VA) of duodenum and jejunum and the crypt depth (CD) of jejunum and ileum of piglets were significantly decreased by PEDV infection (P < 0.05), and ZnO could mitigate these effects (P < 0.05). Moreover, PEDV infection significantly decreased the serum catalase (CAT) activity of piglets (P < 0.05), while it significantly increased by ZnO administration (P < 0.05). The activities of jejunal catalase glutathione peroxidase (GSH-Px), CAT and total superoxide dismutase (T-SOD) of piglets were significantly decreased as well as the duodenal and jejunal malondialdehyde (MDA) content was significantly increased after PEDV infection, while those increased by ZnO administration (P < 0.05). which could be rescued by ZnO administration (P < 0.05). Overall, administration of 100 mg/kg BW ZnO can improve growth performance and intestinal morphology, enhance intestinal barrier function and antioxidant capacity of pigs infected with PEDV.

Key words： zinc oxide; PEDV; growth performance; intestinal barrier function; antioxidant capacity

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