研究论文 RESEARCH PAPER

饲粮中添加岩藻多糖对断奶羔羊器官指数、肝功能指标、肝脏和脾脏抗氧化能力以及脾脏免疫功能的影响

  • 王嘉麟 ,
  • 许悦雯 ,
  • 王胜男 ,
  • 邱晓媛 ,
  • 吴鹏鑫 ,
  • 高振华 ,
  • 赵志辉 ,
  • 于辉 ,
  • 尹福泉
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  • 1. 广东海洋大学滨海农业学院, 湛江 524088;
    2. 佛山科学技术学院生命科学与工程学院, 佛山 528225
王嘉麟(2000-),男,四川成都人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1956181738@qq.com

收稿日期: 2022-04-08

  网络出版日期: 2022-10-17

基金资助

2019年广东省科技厅项目——雷州山羊健康养殖技术集成(K19015);2020年广东省科技专项资金(“大专项”+“任务清单”)项目——雷州山羊保育繁推一体化生态健康养殖关键技术创新与示范;广东省农业农村厅项目——广东省现代草牧业(羊)产业技术体系(2019KJ127);广东省教育厅项目——华南农业大学-广东海洋大学高校服务乡村振兴共同体黑山羊养殖协同服务;2021年省科技专项资金(“大专项+任务清单”)竞争性分配项目——“粤西地区全株青贮玉米种质资源引进、高效生产及饲用价值研究”;2019年广东省重点领域研发计划书项目——中药废弃物资源化利用关键技术研究及示范(2019B110209005)

Effects of Dietary Fucoidan on Organ Indexes, Liver Function Indexes, Liver and Spleen Antioxidant Capacity and Spleen Immune Function of Weaned Goat Kids

  • WANG Jialin ,
  • XU Yuewen ,
  • WANG Shengnan ,
  • QIU Xiaoyuan ,
  • WU Pengxin ,
  • GAO Zhenhua ,
  • ZHAO Zhihui ,
  • YU Hui ,
  • YIN Fuquan
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  • 1. Binhai Agricultural College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. School of Life and Engineering, Foshan University, Foshan 528225, China

Received date: 2022-04-08

  Online published: 2022-10-17

摘要

本试验旨在探究饲粮中添加岩藻多糖对断奶羔羊器官指数、肝功能、肝脏和脾脏抗氧化能力以及脾脏免疫功能的影响。选择60只健康且体重相近的2月龄断奶去势川中黑山羊公羔,随机分为4组,每组15个重复,每个重复1只羊。对照组(CON组)饲喂基础饲粮,试验组(F1、F2和F3组)分别在基础饲粮中添加0.1%、0.3%和0.5%的岩藻多糖。预试期7 d,正试期30 d。结果表明,1)各组之间肝脏和脾脏指数无显著差异(P>0.05)。2)试验第15天,与CON组相比,F1、F2和F3组血清乳酸脱氢酶(LDH)活性显著降低(P<0.05)。试验第30天,与CON组相比,F1、F2和F3组血清谷草转氨酶(AST)活性显著降低(P<0.05),F2和F3组血清LDH活性显著降低(P<0.05)。3)与CON组相比,F1、F2和F3组断奶羔羊肝脏丙二醛(MDA)和过氧化氢(H2O2)含量显著降低(P<0.05),F2和F3组肝脏谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05)。4)与CON组相比,F1、F2和F3组断奶羔羊脾脏H2O2含量显著降低(P<0.05),F2和F3组脾脏过氧化氢酶(CAT)和总超氧化物歧化酶(T-SOD)活性显著提高(P<0.05)。5)与CON组相比,F1、F2和F3组脾脏肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量显著降低(P<0.05),F2和F3组脾脏白细胞介素-1β(IL-1β)含量显著降低(P<0.05),F1、F2和F3组脾脏白细胞介素-2(IL-2)和白细胞介素-10(IL-10)含量显著提高(P<0.05)。综上所述,饲粮中添加岩藻多糖能够改善断奶羔羊的肝功能,提高肝脏和脾脏抗氧化能力,增强脾脏免疫功能。在本试验条件下,饲粮中添加0.3%岩藻多糖效果最佳。

本文引用格式

王嘉麟 , 许悦雯 , 王胜男 , 邱晓媛 , 吴鹏鑫 , 高振华 , 赵志辉 , 于辉 , 尹福泉 . 饲粮中添加岩藻多糖对断奶羔羊器官指数、肝功能指标、肝脏和脾脏抗氧化能力以及脾脏免疫功能的影响[J]. 动物营养学报, 2022 , 34(10) : 6587 -6595 . DOI: 10.3969/j.issn.1006-267x.2022.10.052

Abstract

The purpose of this study was to evaluate the effects of dietary fucoidan on organ indexes, liver function indexes, liver and spleen antioxidant capacity and spleen immune function of weaned goat kids. Sixty 2-month-old weaned castrated male goat kids (Chuanzhong black goats) were randomly allocated to 4 groups with 15 replicates per group and 1 goat per replicate. Goats in the control group (CON group) were fed a basal diet, while others in experimental groups (F1, F2 and F3 groups) were fed the basal diet supplemented with 0.1%, 0.3% and 0.5% fucoidan, respectively. The pre-trial period lasted for 7 days, and the formal trial period lasted for 30 days. The results showed as follows: 1) no significantly differences were observed on liver and spleen indexes among all groups (P>0.05). 2) On day 15, compared with the CON group, the serum dehydrogenase (LDH) activity of F1, F2 and F3 groups was significantly decreased (P < 0.05). On day 30, compared with the CON group, the serum aspartate aminotransferase (AST) activity of F1, F2 and F3 groups was significantly decreased (P < 0.05), and the serum LDH activity of F2 and F3 groups was significantly decreased (P < 0.05). 3) Compared with the CON group, the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in liver of F1, F2 and F3 groups were significantly decreased (P < 0.05), and the liver glutathione peroxidase (GSH-Px) activity of F2 and F3 groups was significantly increased (P < 0.05). 4) Compared with the CON group, the spleen H2O2 content of F1, F2 and F3 groups was significantly decreased (P < 0.05), and the activities of catalase (CAT) and total antioxidant capacity (T-SOD) in spleen of F2 and F3 groups were significantly increased (P < 0.05). 5) Compared with the CON group, the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in spleen of F1, F2 and F3 groups were significantly decreased (P < 0.05), the spleen nterleukin-1β (IL-1β) content of F2 and F3 groups was significantly decreased (P < 0.05), and the contents of interleukin-2 (IL-2) and interleukin-10 (IL-10) in spleen of F1, F2 and F3 groups were significantly increased (P < 0.05). In conclusion, dietary fucoidan has positive effects on liver function of weaned goat kids, improve the liver and spleen antioxidant capacity, enhance the spleen immune function. Besides, in this study, dietary supplementation of 0.3% fucoidan has the better effects.

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