研究论文 RESEARCH PAPER

葛根多糖对健康犊牛基础临床指标、生理生化指标及血浆代谢物的影响

  • 莘余 ,
  • 吕尚揆 ,
  • 尤留超 ,
  • 樊磊 ,
  • 张琼 ,
  • 苏柘僮 ,
  • 曹随忠 ,
  • 余树民 ,
  • 沈留红
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  • 1. 四川农业大学动物医学院, 动物疫病与人类健康四川省重点实验室, 奶牛疾病研究中心, 成都 611130;
    2. 广西英路维特药物有限公司, 荔浦 546600
莘余(1996-),女,河北张家口人,硕士研究生,从事奶牛疾病研究。E-mail:xy15031324924@163.com

收稿日期: 2022-07-14

  网络出版日期: 2022-10-17

基金资助

四川省科技厅科技计划项目(2019YJ0650);四川农业大学本科科研兴趣培养计划(KY2020186)

Effects of Pueraria Polysaccharides on Basic Clinical Indices, Physiological and Biochemical Indices and Plasma Metabolites of Healthy Calves

  • SHEN Yu ,
  • LYU Shangkui ,
  • YOU Liuchao ,
  • FAN Lei ,
  • ZHANG Qiong ,
  • SU Zhetong ,
  • CAO Suizhong ,
  • YU Shumin ,
  • SHEN Liuhong
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  • 1. Medical Research Center for Cow Disease, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. Guangxi Innovate Medical Technology Co., Ltd., Lipu 546600, China

Received date: 2022-07-14

  Online published: 2022-10-17

摘要

本试验旨在研究葛根多糖对健康犊牛基础临床指标、生理生化指标及血浆代谢物的影响。试验选取年龄[(6.20±3.65) d]和体重[(47.09±4.50) kg]相近的健康荷斯坦犊牛24头,随机分为对照组(C组)和试验组(E组),每组12头。E组每天每头牛在牛奶中添加400 mg/kg葛根多糖,连续5 d;C组不添加。观察并记录7 d内试验犊牛体温、心率、呼吸频率及21 d内犊牛体重和腹泻率。检测第1、3、5、7天犊牛血常规、血清生化指标及第7天组间血浆差异代谢物。结果显示:1)葛根多糖对犊牛体温、心率、呼吸频率、血常规指标、血清生化指标和体重均无显著影响(P>0.05)。E组犊牛试验第1~7天、第8~14天、第15~21天体重均高于C组;第8~21天,C组和E组犊牛腹泻率分别为5.36%和0。2)共鉴定出14种血浆差异代谢物,其中具有促进肠道修复及发育作用的二酰甘油和瓜氨酸丰度显著上调(P<0.05),而具有促炎作用的溶血磷脂酰胆碱18 ∶ 0以及参与机体抗氧化调节的胆绿素丰度均显著下调(P<0.05)。由此可见,葛根多糖对健康犊牛无明显临床副作用,可调节健康犊牛机体代谢,促进肠道发育,增强机体抗氧化能力,降低机体炎性反应,从而减少犊牛腹泻,促进犊牛生长。

本文引用格式

莘余 , 吕尚揆 , 尤留超 , 樊磊 , 张琼 , 苏柘僮 , 曹随忠 , 余树民 , 沈留红 . 葛根多糖对健康犊牛基础临床指标、生理生化指标及血浆代谢物的影响[J]. 动物营养学报, 2022 , 34(10) : 6609 -6619 . DOI: 10.3969/j.issn.1006-267x.2022.10.054

Abstract

This experiment was conducted to explore the effects of Pueraria polysaccharides on basic clinical indices, physiological and biochemical indices and plasma metabolites of healthy calves. Twenty-four healthy calves with similar age [(6.20±3.65) d] and body weight [(47.09±4.50) kg] were randomly divided into control group (group C) and test group (group E), each group contained 12 calves. Calves in group E were fed with 400 mg/kg Pueraria polysaccharides in the milk of each cow every day for 5 days, while the others in group C were not added. The body temperature, heart rate, respiratory rate of calves within 7 days, and body weight and diarrhea rate of calves within 21 days were observed and recorded. The routine blood indices, serum biochemical indices on days 1, 3, 5 and 7 and the plasma differential metabolites between two groups on day 7 were detected. The results showed as follows: 1) Pueraria polysaccharides had no significant effects on body temperature, heart rate, respiratory rate, routine blood indices, serum biochemical indices and body weight of calves (P>0.05). During days 1 to 7, days 8 to 14 and days 15 to 21, the body weight of calves of group E was higher than that of group C; during days 8 to 21, the diarrhea rates of calves of group C and group E were 5.36% and 0, respectively. 2) There were 14 differential metabolites were identified, among them, the abundances of diacylglycerol and citrulline which could promote intestinal development were significantly upregulated (P <0.05), while the abundances of lysophosphatidylcholine 18 ∶ 0 and biliverdin were significantly down-regulated (P <0.05), which had proinflammatory effect and involve the body antioxidant regulation, respectively. In conclusion, Pueraria polysaccharides have no significant clinical side effects on healthy calves, and it can regulate metabolism of healthy calves, promote intestinal development, enhance antioxidant capacity and reduce the inflammatory reaction, thus reduce the diarrhea and promote the growth of calves.

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