研究论文 RESEARCH PAPER

大菱鲆和红鳍东方鲀脂肪酸转运蛋白的组织分布及营养调控

  • 赵丽丽 ,
  • 王迪欣 ,
  • 廖章斌 ,
  • 毕清竹 ,
  • 马强 ,
  • 卫育良 ,
  • 梁萌青 ,
  • 乔秀亭 ,
  • 程镇燕 ,
  • 徐后国
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  • 1. 天津农学院水产学院, 天津 300392;
    2. 中国水产科学研究院黄海水产研究所, 青岛 266071
赵丽丽(1998-),女,山东潍坊人,硕士研究生,研究方向为水产动物营养与饲料。E-mail:2627828299@qq.com

收稿日期: 2022-02-14

  网络出版日期: 2022-10-17

基金资助

中央公益性科研机构基础研究基金项目(20603022022004, 2020TD48);财政部和农业农村部农业产业技术体系(CARS-47)

Tissue Distribution and Nutritional Regulation of Fatty Acid Transport Protein in Scophthalmus maximus and Takifugu rubripes

  • ZHAO Lili ,
  • WANG Dixin ,
  • LIAO Zhangbin ,
  • BI Qingzhu ,
  • MA Qiang ,
  • WEI Yuliang ,
  • LIANG Mengqing ,
  • QIAO Xiuting ,
  • CHENG Zhenyan ,
  • XU Houguo
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  • 1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
    2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Received date: 2022-02-14

  Online published: 2022-10-17

摘要

本研究对2种海洋硬骨鱼(大菱鲆和红鳍东方鲀)脂肪酸转运蛋白(FATP)亚型FATP1、FATP4和FATP6进行了序列分析,并对其在不同组织或不同营养状况下的基因表达特征进行了研究。在FATP表达的组织分布研究中,分别取大菱鲆和红鳍东方鲀的不同组织。在饲养试验中,分别饲喂大菱鲆和红鳍东方鲀幼鱼不同脂肪水平(8%、12%和16%)的试验饲料,进行9周的饲养试验,然后进行饥饿试验(30 d)。采用实时荧光定量PCR检测大菱鲆和红鳍东方鲀幼鱼不同组织中以及不同营养状态下FATP mRNA的相对表达量。结果表明: 1)大菱鲆和红鳍东方鲀FATP与其他鱼类和哺乳动物中的同源序列具有高度一致性,但FATP6与FATP1和FATP4的同源性较低。2)大菱鲆FATP1在大脑、心脏、眼睛和肾脏中高表达,红鳍东方鲀FATP1在肝脏中高表达。大菱鲆和红鳍东方鲀FATP4在消化道中高表达。大菱鲆FATP6在幽门盲肠和前肠中高表达,而红鳍东方鲀FATP6仅在肝脏中高表达。3)饲料脂肪水平对大菱鲆和红鳍东方鲀的肝脏FATP1、FATP4和FATP6的mRNA相对表达量无显著影响(P>0.05)。4)大菱鲆第30天的肝脏FATP1的mRNA相对表达量显著高于第0天、第10天和第20天(P<0.05)。红鳍东方鲀第16天和第31天的肝脏FATP1的mRNA相对表达量显著高于第0天、第1天、第4天和第9天(P<0.05),第9天和第31天的肝脏FATP4的mRNA相对表达量显著高于第0天、第1天和第4天(P<0.05),第16天的肝脏FATP6的mRNA相对表达量显著高于第0天、第1天、第4天、第9天和第31天(P<0.05)。由此可见,大菱鲆和红鳍东方鲀FATP表达的组织分布模式不同,但FATP表达对饲料脂肪水平和饥饿时间的响应相似。饲料脂肪水平对大菱鲆和红鳍东方鲀肝脏FATP1、FATP4和FATP6的表达均无显著影响,但饥饿可上调红鳍东方鲀肝脏FATP1、FATP4和FATP6以及大菱鲆肝脏FATP1的表达。

本文引用格式

赵丽丽 , 王迪欣 , 廖章斌 , 毕清竹 , 马强 , 卫育良 , 梁萌青 , 乔秀亭 , 程镇燕 , 徐后国 . 大菱鲆和红鳍东方鲀脂肪酸转运蛋白的组织分布及营养调控[J]. 动物营养学报, 2022 , 34(10) : 6620 -6633 . DOI: 10.3969/j.issn.1006-267x.2022.10.055

Abstract

In the present study, three fatty acid transport protein (FATP) isoforms, namely FATP1, FATP4 and FATP6 in two marine teleosts (Scophthalmus maximus and Takifugu rubripes) were characterized in terms of sequence analysis, as well as the gene expression characteristics in different tissues and different nutritional status were studied. In the study of tissue distribution of FATP expression, the different tissues of Scophthalmus maximus and Takifugu rubripes were selected. In the feeding experiment, juvenile fish of Scophthalmus maximus and Takifugu rubripes were fed experimental diets with different lipid levels (8%, 12% and 16%) for 9 weeks, followed by a starvation experiment (30 d). The FATP mRNA relative expression levels of in different tissues and different nutritional status of Scophthalmus maximus and Takifugu rubripes were detected by real-time fluorescent quantitative PCR. The results showed as follows: 1) the FATP of Scophthalmus maximus and Takifugu rubripes showed high identity to their orthologs in other fish species and mammals, but FATP6 showed a low identity to FATP1 and FATP4. 2) The FATP1 of Scophthalmus maximus was high expression in brain, heart, eye and kidney, and the FATP1 of Takifugu rubripes was high expression in liver. The FATP4 of Scophthalmus maximus and Takifugu rubripes was high expression in digestive tract. The FATP6 of Scophthalmus maximus was high expression in pyloric caecum and anterior intestine, and the e FATP6 of Takifugu rubripes was only high expression in liver. 3) Dietary lipid levels had no significant effects on mRNA relative expression levels of FATP1, FATP4 and FATP6 in liver of Scophthalmus maximus and Takifugu rubripes (P > 0.05). 4) The liver FATP1 mRNA relative expression level of Scophthalmus maximus on day 30 was significantly higher than that on day 0, day 10 and day 20 (P < 0.05). The liver FATP1 mRNA relative expression level of Takifugu rubripes on day 16 and day 31 was significantly higher than that on day 0, day 1, day 4 and day 20 (P < 0.05), the liver FATP4 mRNA relative expression level on day 9 and day 31 was significantly higher than that on day 0, day 1 and day 4 (P < 0.05), and the liver FATP6 mRNA relative expression level on day 16 was significantly higher than that on day 0, day 1, day 4, day 9 and day 31 (P < 0.05). In conclusion, the tissue distribution pattern of FATP expression is different between Scophthalmus maximus and Takifugu rubripes, however, the response of FATP expression to diet lipid level and starvation time is similar. Dietary lipid levels had no significant effects on expression of FATP1, FATP4 and FATP6 in liver of Scophthalmus maximus and Takifugu rubripes, however, the starvation can up-regulate the expression of FATP1, FATP4, FATP6 in liver of Scophthalmus maximus and FATP6 in liver of Takifugu rubripes.

参考文献

[1] HALL A M,SMITH A J,BERNLOHR D A.Characterization of the acyl-CoA synthetase activity of purified murine fatty acid transport protein 1[J].The Journal of Biological Chemistry,2003,278(44):43008-43013.  
[2] POHL J,RING A,HERMANN T,et al.Role of FATP in parenchymal cell fatty acid uptake[J].Biochimica et Biophysica Acta,2004,1686(1/2):1-6.
[3] BLACK P N,DIRUSSO C C.Vectorial acylation:linking fatty acid transport and activation to metabolic trafficking[J].Novartis Foundation Symposium,2007,286:127-138.
[4] RICHARDS M R,HARP J D,ORY D S,et al.Fatty acid transport protein 1 and long-chain acyl coenzyme A synthetase 1 interact in adipocytes[J].Journal of Lipid Research,2006,47(3):665-672.  
[5] QIAN S G,FUJII T,ITO K,et al.Cloning and functional characterization of a fatty acid transport protein (FATP) from the pheromone gland of a lichen moth,Eilema japonica,which secretes an alkenyl sex pheromone[J].Insect Biochemistry and Molecular Biology,2011,41(1):22-28.  
[6] TODORCEVIĆMŃ,VEGUSDAL A,GJØEN T,et al.Changes in fatty acids metabolism during differentiation of Atlantic salmon preadipocytes;effects of n-3 and n-9 fatty acids[J].Biochimica et Biophysica Acta,2008,1781(6/7):326-335.
[7] YAO J P,HU P C,ZHU Y H,et al.Lipid-lowering effects of lotus leaf alcoholic extract on serum,hepatopancreas,and muscle of juvenile grass carp via gene expression[J].Frontiers in Physiology,2020,11:584782.
[8] 毕清竹,梁萌青,廖章斌,等.饲料中胆汁酸对红鳍东方鲀脂肪酸组成及抗氧化能力的影响[J].上海海洋大学学报,2020,29(6):829-839. BI Q Z,LIANG M Q,LIAO Z B,et al.Effect of dietary bile acid supplementation on fatty acid composition and anti-oxidative capacity of tiger puffer Takifugu rubripes[J].Journal of Shanghai Ocean University,2020,29(6):829-839.(in Chinese)
[9] XU H G,ZHANG Y Q,WANG C Q,et al.Cloning and characterization of fatty acid transport proteins in Japanese seabass Lateolabrax japonicus,and their gene expressions in response to dietary arachidonic acid[J].Aquaculture Research,2017,48(12):5718-5728.  
[10] XU H G,BI Q Z,PRIBYTKOVA E,et al.Different lipid scenarios in three lean marine teleosts having different lipid storage patterns[J].Aquaculture,2021,536:736448.
[11] XU H G,TURCHINI G M,FRANCIS D S,et al.Are fish what they eat?A fatty acid's perspective[J].Progress in Lipid Research,2020,80:101064.
[12] REGOST C,ARZEL J,CARDINAL M,et al.Dietary lipid level,hepatic lipogenesis and flesh quality in turbot (Psetta maxima)[J].Aquaculture,2001,193(3/4):291-309.
[13] MA X H,BI Q Z,KONG Y Y,et al.Dietary lipid levels affected antioxidative status,inflammation response,apoptosis and microbial community in the intestine of juvenile turbot (Scophthalmus maximus L.)[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2022,264:111118.
[14] XU H G,MU Y C,ZHANG Y,et al.Graded levels of fish protein hydrolysate in high plant diets for turbot (Scophthalmus maximus):effects on growth performance and lipid accumulation[J].Aquaculture,2016,454:140-147.
[15] KIKUCHI K,FURUTA T,IWATA N,et al.Effect of dietary lipid levels on the growth, feed utilization,body composition and blood characteristics of tiger puffer Takifugu rubripes[J].Aquaculture,2009,298(1/2):111-117.
[16] TAKII K,UKAWA M,NAKAMURA M,et al.Suitable lipid level in brown fish meal diet for tiger puffer[J].Fisheries Science,1995,61(5):841-844.  
[17] LIAO Z B,SUN Z Y,BI Q Z,et al.Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions[J].Fish Physiology and Biochemistry,2021,47(6):1739-1758.  
[18] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408.  
[19] ANDERSON C M,STAHL A.SLC27 fatty acid transport proteins[J].Molecular Aspects of Medicine,2013,34(2/3):516-528.
[20] LEWIS S E,LISTENBERGER L L,ORY D S,et al.Membrane topology of the murine fatty acid transport protein 1[J].Journal of Biological Chemistry,2001,276(40):37042-37050.  
[21] MILGER K,HERRMANN T,BECKER C,et al.Cellular uptake of fatty acids driven by the ER-localized acyl-CoA synthetase FATP4[J].Journal of Cell Science,2006,119(Pt 22):4678-4688.
[22] STUHLSATZ-KROUPER S M,BENNETT N E,SCHAFFER J E.Molecular aspects of fatty acid transport:mutations in the IYTSGTTGXPK motif impair fatty acid transport protein function[J].Prostaglandins,Leukotrienes,and Essential Fatty Acids,1999,60(5/6):285-289.
[23] ORDOVÁS L,ROY R,ZARAGOZA P,et al.Structural and functional characterization of the bovine solute carrier family 27 member 1(SLC27A1) gene[J].Cytogenetic and Genome Research,2006,115(2):115-122.  
[24] BINNERT C,KOISTINEN H A,MARTIN G,et al.Fatty acid transport protein-1 mRNA expression in skeletal muscle and in adipose tissue in humans[J].American Journal of Physiology.Endocrinology and Metabolism,2000,279(5):E1072-E1079.
[25] WU Q W,ORTEGON A M,TSANG B,et al.FATP1 is an insulin-sensitive fatty acid transporter involved in diet-induced obesity[J].Molecular and Cellular Biology,2006,26(9):3455-3467.  
[26] TORSTENSEN B E,NANTON D A,OLSVIK P A,et al.Gene expression of fatty acid-binding proteins,fatty acid transport proteins (CD36 and FATP) and β-oxidation-related genes in Atlantic salmon (Salmo salar L.) fed fish oil or vegetable oil[J].Aquaculture Nutrition,2009,15(4):440-451.  
[27] KANEKO G,YAMADA T,HAN Y N,et al.Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream[J].General and Comparative Endocrinology,2013,184:51-60.
[28] STAHL A.A current review of fatty acid transport proteins (SLC27)[J].Pflugers Archiv:European Journal of Physiology,2004,447(5):722-727.  
[29] LENZ L S,MARX J,CHAMULITRAT W,et al.Adipocyte-specific inactivation of Acyl-CoA synthetase fatty acid transport protein 4(FATP4) in mice causes adipose hypertrophy and alterations in metabolism of complex lipids under high fat diet[J].Journal of Biological Chemistry,2011,286(41):35578-35587.  
[30] SHIM J,MOULSON C L,NEWBERRY E P,et al.Fatty acid transport protein 4 is dispensable for intestinal lipid absorption in mice[J].Journal of Lipid Research,2009,50(3):491-500.  
[31] MOULSON C L,LIN M H,WHITE J M,et al.Keratinocyte-specific expression of fatty acid transport protein 4 rescues the wrinkle-free phenotype in Slc27a4/FATP4 mutant mice[J].Journal of Biological Chemistry,2007,282(21):15912-15920.  
[32] FITSCHER B A,RIEDEL H D,YOUNG K C,et al.Tissue distribution and cDNA cloning of a human fatty acid transport protein (hsFATP4)[J].Biochimica et Biophysica Acta,1998,1443(3):381-385.  
[33] HERRMANN T,BUCHKREMER F,GOSCH I,et al.Mouse fatty acid transport protein 4(FATP4):characterization of the gene and functional assessment as a very long chain acyl-CoA synthetase[J].Gene,2001,270(1/2):31-40.
[34] MOHD-YUSOF N Y,MONROIG O,MOHD-ADNAN A,et al.Investigation of highly unsaturated fatty acid metabolism in the Asian sea bass,Lates calcarifer[J].Fish Physiology and Biochemistry,2010,36(4):827-843.  
[35] GIMENO R E,ORTEGON A M,PATEL S,et al.Characterization of a heart-specific fatty acid transport protein[J].Journal of Biological Chemistry,2003,278(18):16039-16044.  
[36] MAROTTA M,FERRER-MARTNEZ A,PARNAU J,et al.Fiber type-and fatty acid composition-dependent effects of high-fat diets on rat muscle triacylglyceride and fatty acid transporter protein-1 content[J].Metabolism,2004,53(8):1032-1036.  
[37] 陈培赟,任潇潇,毕保良.饲料中添加黄藤素对吉富罗非鱼生长性能、抗氧化能力和非特异性免疫的影响[J].上海海洋大学学报,2021,30(5):812-820. CHEN P Y,REN X X,BI B L.Effects of dietary palmatine on growth performance,antioxidant capacity and non-specific immunity of GIFT strain of Nile tilapia (Oreochromis niloticus)[J].Journal of Shanghai Ocean University,2021,30(5):812-820.(in Chinese)
[38] DU J L,CAO L P,JIA R,et al.Alleviative effects of total flavones of Glycyrrhiza uralensis Fisch on oxidative stress and lipid metabolism disorder induced by high-fat diet in intestines of Tilapia (Oreochromis niloticus)[J].3 Biotech,2021,11(7):348.
[39] YAN J,LIAO K,MAI K S,et al.Dietary lipid levels affect lipoprotein clearance,fatty acid transport,lipogenesis and lipolysis at the transcriptional level in muscle and adipose tissue of large yellow croaker (Larimichthys crocea)[J].Aquaculture Research,2017,48(7):3925-3934.  
[40] WANG Y L,MENG R,XU X R,et al.Effects of nutritional status and diet composition on fatty acid transporters expression in zebrafish (Danio rerio)[J].Aquaculture Research,2019,50(3):904-914.  
[41] MAN M Z,HUI T Y,SCHAFFER J E,et al.Regulation of the murine adipocyte fatty acid transporter gene by insulin[J].Molecular Endocrinology,1996,10(8):1021-1028.
[42] MEMON R A,FEINGOLD K R,MOSER A H,et al.Regulation of fatty acid transport protein and fatty acid translocase mRNA levels by endotoxin and cytokines[J].The American Journal of Physiology,1998,274(2):E210-E217.
[43] XIE D Z,WANG M,WANG S Q,et al.Fat powder can be a feasible lipid source in aquafeed for the carnivorous marine teleost golden pompano,Trachinotus ovatus[J].Aquaculture International,2020,28(3):1153-1168.  
[44] RIERA-HEREDIA N,LUTFI E,GUTIÉRREZ J,et al.Fatty acids from fish or vegetable oils promote the adipogenic fate of mesenchymal stem cells derived from gilthead sea bream bone potentially through different pathways[J].PLoS One,2019,14(4):e0215926.
[45] LI X S,CHEN Q C,LI Q F,et al.Effects of high levels of dietary linseed oil on the growth performance,antioxidant capacity,hepatic lipid metabolism,and expression of inflammatory genes in large yellow croaker (Larimichthys crocea)[J].Frontiers in Physiology,2021,12:631850.
[46] WU L X,WEI C C,YANG S B,et al.Effects of fat and fatty acids on the formation of autolysosomes in the livers from yellow catfish Pelteobagrus fulvidraco[J].Genes,2019,10(10):751.
[47] LEI C X,LI M M,TIAN J J,et al.Transcriptome analysis of golden pompano (Trachinotus ovatus) liver indicates a potential regulatory target involved in HUFA uptake and deposition[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics,2020,33:100633.
[48] ZHU Y H,TAN Q S,ZHANG L S,et al.The migration of docosahexenoic acid (DHA) to the developing ovary of female zebrafish (Danio rerio)[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2019,233:97-105.
[49] MASHEK D G,COLEMAN R A.Cellular fatty acid uptake:the contribution of metabolism[J].Current Opinion in Lipidology,2006,17(3):274-278.  
[50] HOLLOWAY G P,CHOU C J,LALLY J,et al.Increasing skeletal muscle fatty acid transport protein 1(FATP1) targets fatty acids to oxidation and does not predispose mice to diet-induced insulin resistance[J].Diabetologia,2011,54(6):1457-1467.  
[51] TORSTENSEN B E,STUBHAUG I.Beta-oxidation of 18:3n-3 in Atlantic salmon (Salmo salar L.) hepatocytes treated with different fatty acids[J].Lipids,2004,39(2):153-160.  
[52] LIAO K,MENG R,RAN Z S,et al.Short-term starvation in silver pomfret (Pampus argenteus):molecular effects on lipid mobilization and utilization[J].Aquaculture Research,2017,48(9):4874-4885.  
[53] SÁNCHEZ-GURMACHES J,CRUZ-GARCIA L,GUTIÉRREZ J,et al.mRNA expression of fatty acid transporters in rainbow trout:in vivo and in vitro regulation by insulin,fasting and inflammation and infection mediators[J].Comparative Biochemistry and Physiology.Part A,Molecular&Integrative Physiology,2012,163(2):177-188.  
[54] SÁNCHEZ-GURMACHES J,ØSTBYE T K,NAVARRO I,et al.In vivo and in vitro insulin and fasting control of the transmembrane fatty acid transport proteins in Atlantic salmon (Salmo salar)[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2011,301(4):R947-R957.
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