研究论文 RESEARCH PAPER

饲料中添加枯草芽孢杆菌HSP05对凡纳滨对虾生长性能、抗病力和非特异性免疫力的影响

  • 于道德 ,
  • 陈胜林 ,
  • 郭少菁 ,
  • 樊英 ,
  • 王晓璐 ,
  • 宋静静 ,
  • 刘洪军
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  • 1. 山东省海洋科学研究院, 青岛 266104;
    2. 滨州市海洋发展研究院, 滨州 256603;
    3. 山东省海水养殖病害防治重点实验室, 青岛 266104
于道德(1978-),男,山东青岛人,博士,研究方向为水产动物营养与繁育。E-mail:wensentte@163.com

收稿日期: 2022-02-26

  网络出版日期: 2022-10-17

基金资助

山东省现代农业产业技术体系虾蟹类创新团队项目(创新团队SDAIT-13-1号)

Effects of Dietary Bacillus subtilis HSP05 on Growth Performance, Disease Resistance and Non-Specific Immunity of Litopenaeus vannamei

  • YU Daode ,
  • CHEN Shenglin ,
  • GUO Shaojing ,
  • FAN Ying ,
  • WANG Xiaolu ,
  • SONG Jingjing ,
  • LIU Hongjun
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  • 1. Marine Science Research Institute of Shandong Province, Qingdao 266104, China;
    2. Binzhou Ocean Development Research Institute, Binzhou 256603;
    3. Key Laboratory of Mariculture Disease Control of Shandong Province, Qingdao 266104, China

Received date: 2022-02-26

  Online published: 2022-10-17

摘要

本试验旨在研究饲料中添加枯草芽孢杆菌(Bacillus subtilis) HSP05对凡纳滨对虾(Litopenaeus vannamei)生长性能、抗病力和非特异性免疫力的影响。挑选大小一致的1 200尾凡纳滨对虾虾苗,随机分为4组,每组3个重复,每个重复100尾。各组对虾分别投喂枯草芽孢杆菌HSP05添加量为0(对照组)、105(BS1组)、107(BS2组)、109 CFU/g(BS3组)的试验饲料。试验期4周。结果表明: 1) BS1、BS2和BS3组的终末体重、终末体长、增重率和特定生长率显著高于对照组(P < 0.05)。2) BS1、BS2和BS3组的肝胰腺蛋白酶、淀粉酶活性显著高于对照组(P < 0.05), BS1、BS2和BS3组的肝胰腺谷丙转氨酶和谷草转氨酶活性均显著低于对照组(P < 0.05)。3) BS1、BS2和BS3组的血清酚氧化酶和溶菌酶活性显著高于对照组(P < 0.05)。BS1、BS2和BS3组的肝胰腺过氧化氢酶活性显著高于对照组(P < 0.05), BS2和BS3组的肝胰腺超氧化物歧化酶活性显著高于对照组(P < 0.05)。4) BS1、BS2和BS3组的血细胞吞噬率显著高于对照组(P < 0.05), BS1、BS2和BS3组的呼吸爆发活性显著低于对照组(P < 0.05)。5)副溶血弧菌侵染持续7 d时,对照组的存活率为46.67%,而BS1、BS2和BS3组的存活率均达到82.67%以上。由此可见,饲料中添加枯草芽孢杆菌HSP05可促进凡纳滨对虾生长,增强免疫力,提高抗病力。枯草芽孢杆菌HSP05的添加量为107 CFU/g时,凡纳滨对虾的生长表现最优,非特异性免疫得到有效提高。

本文引用格式

于道德 , 陈胜林 , 郭少菁 , 樊英 , 王晓璐 , 宋静静 , 刘洪军 . 饲料中添加枯草芽孢杆菌HSP05对凡纳滨对虾生长性能、抗病力和非特异性免疫力的影响[J]. 动物营养学报, 2022 , 34(10) : 6634 -6642 . DOI: 10.3969/j.issn.1006-267x.2022.10.056

Abstract

This experiment was conducted to study the effects of dietary Bacillus subtilis HSP05 on growth performance, disease resistance and non-specific immunity of Litopenaeus vannamei. A total of 1 200 Litopenaeus vannamei young shrimp with the same size were randomly divided into 4 groups with 3 replicates per group and 100 shrimp per replicate. Prawns in 4 groups were fed experimental diets supplemented with 0 (control group), 105 (BS1 group), 107 (BS2 group) and 109 CFU/g (BS3 group) Bacillus subtilis HSP05, respectively. The experiment lasted for 4 weeks. The results showed as follows: 1) the final body weight, final body length, weight gain rate and specific growth rate of BS1, BS2 and BS3 groups were significantly higher than those of the control group (P < 0.05). 2) The activities of protease and amylase in hepatopancreas of BS1, BS2 and BS3 groups were significantly higher than those of the control group (P < 0.05), and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in hepatopancreas of BS1, BS2 and BS3 groups were significantly lower than those of the control group (P < 0.05). 3) The activities of phenol oxidase and lysozyme in serum of BS1, BS2 and BS3 groups were significantly higher than those of the control group (P < 0.05). The hepatopancreas catalase activity of BS1, BS2 and BS3 groups was significantly higher than that of the control group (P < 0.05), and the hepatopancreas superoxide dismutase activity of BS2 and BS3 groups was significantly higher than that of the control group (P < 0.05). 4) The phagocytosis rate of blood cell of BS1, BS2 and BS3 groups was significantly higher than that of the control group (P < 0.05), and the respiratory burst activity of BS1, BS2 and BS3 groups was significantly lower than that of the control group (P < 0.05). 5) After infection with Vibrio parahaemolyticus 7 days, the survival rate of the control group was 46.67%, and the survival rate of BS1, BS2 and BS3 groups was more than 82.67%. In conclusion, dietary Bacillus subtilis HSP05 can promote the growth of Litopenaeus vannamei, and enhance immunity and disease resistance. When dietary adding 107 CFU/g Bacillus subtilis HSP05, the growth shows the best, and effectively improve the non-specific immune.

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