研究论文 RESEARCH PAPER

饲粮添加粪肠球菌对生长期乌苏里貉生长性能、氮代谢、血清生化指标及粪便菌群的影响

  • 白银龙 ,
  • 牛一兵 ,
  • 张海华 ,
  • 巩文洋 ,
  • 曲毅程 ,
  • 龙娟 ,
  • 王夕国 ,
  • 巩元芳 ,
  • 赵惠媛 ,
  • 李素芬 ,
  • 冯敏山
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  • 1. 河北科技师范学院, 秦皇岛 066004;
    2. 秦皇岛市三晶新农饲料有限公司, 昌黎 066600;
    3. 河北省碣石皮毛产业研究院, 昌黎 066600
白银龙(1995-),男,河北保定人,硕士研究生,特种动物养殖专业。E-mail:bai406931548@126.com

收稿日期: 2022-02-26

  网络出版日期: 2022-10-17

基金资助

河北省重点研发计划项目(162366051D-8);秦皇岛市科技创新平台建设运行经费(201702B003);河北省重点研发计划项目(21327308D); 河北省特色动物种质资源挖掘与创新重点实验室项目; 河北科技师范学院动物科技学院资助项目(DK202103)

Effects of Dietary Enterococcus faecalis on Growth Performance, Nitrogen Metabolism, Serum Biochemical Indices and Fecal Microbiota of Growing Ussuri Raccoon Dogs

  • BAI Yinlong ,
  • NIU Yibing ,
  • ZHANG Haihua ,
  • GONG Wenyang ,
  • QU Yicheng ,
  • LONG Juan ,
  • WANG Xiguo ,
  • GONG Yuanfang ,
  • ZHAO Huiyuan ,
  • LI Sufen ,
  • FENG Minshan
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  • 1. Hebei Normal University of Science and Technology, Qinhuangdao 066004, China;
    2. Qinhuangdao Sanjing Xinnong Feed Co., Ltd., Changli 066600, China;
    3. Hebei Jieshi Fur Industry Research Institute, Changli 066600, China

Received date: 2022-02-26

  Online published: 2022-10-17

摘要

本试验旨在研究饲粮添加不同水平粪肠球菌对生长期乌苏里貉生长性能、氮代谢、血清生化指标及粪便菌群的影响,以确定粪肠球菌的适宜添加水平及作用机制。采用单因素完全随机试验设计,选择70日龄、体重[(2.44±0.24) kg]相近的健康乌苏里貉公貉40只,随机分成4个组,每组10只。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮基础上添加1×108(Ⅰ组)、1×109(Ⅱ组)和1×1010 CFU/kg(Ⅲ组)粪肠球菌的饲粮。试验预试期7 d,正试期70 d。结果表明: 1)与对照组相比, Ⅰ组和Ⅱ组平均日增重显著提高(P < 0.05),且Ⅰ组显著高于Ⅱ组(P < 0.05);Ⅰ组和Ⅱ组料重比显著降低(P < 0.05);Ⅲ组上述2项指标无显著差异(P>0.05)。2)与对照组相比, 3个粪肠球菌添加组氮沉积量以及Ⅰ组和Ⅱ组蛋白质生物学价值显著提高(P < 0.05)。3)与对照组相比, Ⅰ组和Ⅱ组血清白蛋白含量显著提高(P < 0.05), 3个粪肠球菌添加组血清尿素氮含量显著提高(P < 0.05)。4)与对照组相比, Ⅰ组粪便菌群ACE指数和Chao1指数显著提高(P < 0.05),拟杆菌门和巨球型菌属相对丰度显著提高(P < 0.05),小杆菌属、Catenisphaera和光冈菌属相对丰度显著降低(P < 0.05)。由此可见,饲粮添加粪肠球菌可以提高生长期乌苏里貉生长性能和氮利用率,促进有益菌增殖,抑制潜在病原菌生长,且添加水平以1×108 CFU/kg较为适宜。

本文引用格式

白银龙 , 牛一兵 , 张海华 , 巩文洋 , 曲毅程 , 龙娟 , 王夕国 , 巩元芳 , 赵惠媛 , 李素芬 , 冯敏山 . 饲粮添加粪肠球菌对生长期乌苏里貉生长性能、氮代谢、血清生化指标及粪便菌群的影响[J]. 动物营养学报, 2022 , 34(10) : 6687 -6694 . DOI: 10.3969/j.issn.1006-267x.2022.10.061

Abstract

This experiment was conducted to investigate the effects of different dietary levels of Enterococcus faecalis on the growth performance, nitrogen metabolism, serum biochemical indices and fecal microbiota of growing Ussuri raccoon dogs, so as to determine the optimal dietary level of Enterococcus faecalis and its mechanism. A total of 40 male healthy Ussuri raccoon dogs of 70-day-old with similar body weight of (2.44±0.24) kg were randomly divided into 4 groups with 10 replicates per group, and fed the basal diet (control group) or the basal diet supplemented with 1×108 (group Ⅰ), 1×109 (group Ⅱ) and 1×1010 CFU/kg (group Ⅲ) Enterococcus faecalis, respectively. The trial lasted for 70 days after a 7-day adaption. The results showed as follows: 1) compared with the control group, the average daily gain in groups Ⅰ and Ⅱ was significantly increased (P < 0.05), and which in group Ⅰ was significantly higher than that in group Ⅱ (P < 0.05); the feed to gain ratio in groups Ⅰ and Ⅱ was significantly decreased (P < 0.05); there were no significant differences in the above two indices in group Ⅲ (P>0.05). 2) Compared with the control group, the nitrogen deposition in all Enterococcus faecalis supplemental groups and the protein biological value in groups Ⅰ and Ⅱ were significantly increased (P < 0.05). 3) Compared with the control group, the serum albumin content in groups Ⅰ and Ⅱ was significantly increased (P < 0.05), and the serum urea nitrogen content in the three Enterococcus faecalis supplemental groups was significantly increased (P < 0.05). 4) Compared with the control group, the ACE index and Chao1 index of fecal microbiota in group Ⅰ were significantly increased (P < 0.05), the relative abundances of Bacteroidetes and Megasphaera were significantly increased (P < 0.05), and the relative abundances of Dialister, Catenisphaera and Mitsuokella were significantly decreased (P < 0.05). The results suggested that dietary Enterococcus faecalis can improve the growth performance and nitrogen utilization of growing Ussuri raccoon dogs, promote beneficial bacteria and inhibit potential pathogens; the optimal dietary level is 1×108 CFU/kg.

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