研究论文 RESEARCH PAPER

霉菌毒素吸附剂与有机酸或植物精油的复合物对蛋鸡肠道黏膜屏障功能的影响

  • 陈继发 ,
  • 张佳鑫
展开
  • 宜春学院生命科学与资源环境学院, 江西省高等学校硒农业工程技术研究中心, 宜春 336000
陈继发(1992—),男,湖南邵阳人,讲师,博士,主要从事单胃动物营养与饲料科学研究。E-mail:jifachen66@yeah.net

收稿日期: 2021-11-18

  网络出版日期: 2022-11-14

基金资助

江西省教育厅科技项目(GJJ211624);宜春学院引进高层次人才科研启动基金(3360119022)

Effects of Mycotoxin Adsorbent-Organic Acid Complex and Mycotoxin Adsorbent-Essential Oil Complex on Intestinal Mucosal Barrier Function of Laying Hens

  • CHEN Jifa ,
  • ZHANG Jiaxin
Expand
  • Selenium Agricultural Engineering Technology Research Center of Jiangxi Provincial Colleges and Universities, College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China

Received date: 2021-11-18

  Online published: 2022-11-14

摘要

本试验旨在研究霉菌毒素吸附剂(MA)与有机酸(OA)或植物精油(EO)的复合物对蛋鸡肠道黏膜屏障功能的影响。选取270只健康的29周龄罗曼蛋鸡,随机分为3组,每组6个重复,每个重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.65 g/kg MA-OA复合物、0.70 g/kg MA-EO复合物的试验饲粮。预试期1周,正试期10周。结果表明:与对照组相比,饲粮添加MA-OA复合物或MA-EO复合物显著降低了回肠产气荚膜梭菌相对数量(P<0.05),显著下调了回肠黏膜髓样分化因子88和白细胞介素-1β mRNA相对表达水平(P<0.05),显著提高了回肠蔗糖酶活性和绒毛高度/隐窝深度值(P<0.05);饲粮添加MA-EO复合物显著上调了回肠黏膜闭合蛋白-5(claudin-5) mRNA相对表达水平(P<0.05);饲粮添加MA-OA复合物显著降低了回肠沙门氏菌相对数量(P<0.05),显著下调了回肠黏膜核转录因子-κB p65 mRNA相对表达水平(P<0.05),显著提高了回肠碱性磷酸酶活性和空肠绒毛高度/隐窝深度值(P<0.05)。由此可见,饲粮添加MA-OA复合物或MA-EO复合物均可提高蛋鸡的肠道屏障功能,改善肠道健康。

本文引用格式

陈继发 , 张佳鑫 . 霉菌毒素吸附剂与有机酸或植物精油的复合物对蛋鸡肠道黏膜屏障功能的影响[J]. 动物营养学报, 2022 , 34(11) : 6994 -7001 . DOI: 10.3969/j.issn.1006-267x.2022.11.020

Abstract

This study was conducted to investigate the effects of mycotoxin adsorbent (MA)-organic acid (OA) complex and MA-essential oil (EO) complex on intestinal mucosal barrier function of laying hens. A total of 270 healthy 29-week-old laying hens were randomly divided into 3 groups with 6 replicates per group and 15 hens per replicate. Hens in the control group were fed a basal diet, and hens in the test groups were fed the basal diet supplemented with 0.65 g/kg MA-OA complex and 0.70 g/kg MA-EO complex, respectively. The adjustment period lasted for 1 week, and the experimental period lasted for 10 weeks. The results showed as follows:compared with the control group, dietary supplemented with both MA-OA complex or MA-EO complex significantly reduced the relative amount of Clostridium perfringens in ileum (P<0.05), and significantly down-regulated the mRNA relative expression levels of myeloid differentiation factor 88 (MyD88) and interleukin-1β (IL-1β), while significantly increased the sucrase activity and villus height/crypt depth ratio in ileum (P<0.05). Besides, dietary supplemented with MA-EO complex significantly up-regulated the mRNA relative expression level of claudin-5 in ileal mucosa (P<0.05); dietary supplemented with MA-OA complex significantly reduced the relative amount of Salmonella in ileum (P<0.05), and significantly down-regulated the mRNA relative expression level of nuclear factor kappa B p65 (NF-κB p65) in ileal mucosa (P<0.05), while significantly enhanced the alkaline phosphatase activity in ileum and villus height/crypt depth ratio in jejunum (P<0.05). In conclusion, dietary supplemented with MA-OA complex or MA-EO complex can improve intestinal mucosal barrier function, which contributes to the health of the intestine in laying hens.

参考文献

[1] OKUMURA R, TAKEDA K.Maintenance of intestinal homeostasis by mucosal barriers[J].Inflammation and Regeneration, 2018, 38:5.
[2] PRASAI T P, WALSH K B, BHATTARAI S P, et al.Biochar, bentonite and zeolite supplemented feeding of layer chickens alters intestinal microbiota and reduces campylobacter load[J].PLoS One, 2016, 11(4):e0154061.
[3] CHEN J F, XU M M, KANG K L, et al.The effects and combinational effects of Bacillus subtilis and montmorillonite on the intestinal health status in laying hens[J].Poultry Science, 2020, 99(3):1311-1319.  
[4] MCKNIGHT L L, PEPPLER W, WRIGHT D C, et al.A blend of fatty acids, organic acids, and phytochemicals induced changes in intestinal morphology and inflammatory gene expression in coccidiosis-vaccinated broiler chickens[J].Poultry Science, 2019, 98(10):4901-4908.  
[5] 曾子悠, 苟清碧, 古长松, 等.饲粮添加植物精油和酸化剂组合对麻黄肉鸡生长性能、肠道健康和肉品质的影响[J].动物营养学报, 2021, 33(6):3237-3249. ZENG Z Y, GOU Q B, GU C S, et al.Effects of dietary plant essential oil and acidifier combination on growth performance, intestinal health and meat quality of Mahuang broilers[J].Chinese Journal of Animal Nutrition, 2021, 33(6):3237-3249.(in Chinese)
[6] 陈继发, 耿晓峰.霉菌毒素吸附剂-有机酸复合物和霉菌毒素吸附剂-植物精油复合物对蛋鸡生产性能、蛋品质及血浆激素、抗氧化和免疫指标的影响[J].动物营养学报, 2020, 32(12):5667-5675. CHEN J F, GENG X F.Effects of mycotoxin adsorbent-organic acid complex and mycotoxin adsorbent-essential oil complex on laying performance, egg quality and plasma hormone, antioxidant and immune indices of laying hens[J].Chinese Journal of Animal Nutrition, 2020, 32(12):5667-5675.(in Chinese)
[7] 陈继发.霉菌毒素吸附剂与有机酸或植物精油的复合物对蛋鸡饲粮养分表观消化率及肠道微生物的影响[J].中国畜牧杂志, 2021, 57(12):239-243. CHEN J F.Effects of mycotoxin adsorbent-organic acid complex and mycotoxin adsorbent-essential oil complex on apparent utilization of nutritions and intestinal microflora of laying hens[J].Chinese Journal of Animal Science, 2021, 57(12):239-243.(in Chinese)
[8] CHEN J F, LIU X, QU X Y, et al.Montmorillonite improved the intestinal mucosal barrier functions of laying hens in late production[J].Journal of Animal Physiology and Animal Nutrition, 2019, 103(4):1081-1089.  
[9] 陈继发, 匡佑华, 康克浪, 等.蒙脱石、枯草芽孢杆菌及其互作对蛋鸡血浆激素含量、卵巢繁殖相关基因表达和肠道微生物的影响[J].动物营养学报, 2019, 31(1):294-303. CHEN J F, KUANG Y H, KANG K L, et al.Effects of montmorillonite, Bacillus subtilis and their interaction on hormone contents in plasma, reproduction related genes expression in ovarian and intestinal microflora of laying hens[J].Chinese Journal of Animal Nutrition, 2019, 31(1):294-303.(in Chinese)
[10] CHEN X L, WANG J K, WU Y M, et al.Effects of chemical treatments of rice straw on rumen fermentation characteristics, fibrolytic enzyme activities and populations of liquid- and solid-associated ruminal microbes in vitro[J].Animal Feed Science and Technology, 2008, 141(1/2):1-14.
[11] 陈继发.蒙脱石及其与枯草芽孢杆菌联用对蛋鸡生产性能和肠道功能的影响[D].博士学位论文.长沙:湖南农业大学, 2019. CHEN J F.Effects of montmorillonite and its combination with Bacillus subtilis on production performance and intestinal function of laying hens[D].Ph.D.Thesis.Changsha:Hunan Agricultural University, 2019.(in Chinese)
[12] WANG H, LIANG S S, LI X Y, et al.Effects of encapsulated essential oils and organic acids on laying performance, egg quality, intestinal morphology, barrier function, and microflora count of hens during the early laying period[J].Poultry Science, 2019, 98(12):6751-6760.  
[13] GONG H J, YANG Z Q, CELI P, et al.Effect of benzoic acid on production performance, egg quality, intestinal morphology, and cecal microbial community of laying hens[J].Poultry Science, 2021, 100(1):196-205.  
[14] PRZYBYLSKA-GORNOWICZ B, LEWCZUK B, PRUSIK M, et al.The effects of deoxynivalenol and zearalenone on the pig large intestine.A light and electron microscopy study[J].Toxins, 2018, 10(4):148.
[15] JIA R, MA Q G, FAN Y, et al.The toxic effects of combined aflatoxins and zearalenone in naturally contaminated diets on laying performance, egg quality and mycotoxins residues in eggs of layers and the protective effect of Bacillus subtilis biodegradation product[J].Food and Chemical Toxicology, 2016, 90:142-150.
[16] 周建川, 史东辉, 计成.玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇对动物毒性的研究进展[J].动物营养学报, 2020, 32(6):2460-2466. ZHOU J C, SHI D H, JI C.Research progress on toxicity of zearalenone and deoxynivalenol in animals[J].Chinese Journal of Animal Nutrition, 2020, 32(6):2460-2466.(in Chinese)
[17] XIE K, HE X, HU G L, et al.The preventive effect and mechanisms of adsorbent supplementation in low concentration aflatoxin B1 contaminated diet on subclinical symptom and histological lesions of broilers[J].Poultry Science, 2022, 101(3):101634.
[18] WAN X L, YANG Z B, YANG W R, et al.Toxicity of increasing aflatoxin B1 concentrations from contaminated corn with or without clay adsorbent supplementation in ducklings[J].Poultry Science, 2013, 92(5):1244-1253.  
[19] PIOTROWSKA M, SLIŻEWSKA K, NOWAK A, et al.The effect of experimental fusarium mycotoxicosis on microbiota diversity in porcine ascending colon contents[J].Toxins, 2014, 6(7):2064-2081.  
[20] JOHNSTON D G W, CORR S C.Toll-like receptor signalling and the control of intestinal barrier function[J].Methods in Molecular Biology, 2016, 1390:287-300.
[21] DENG Z T, HAN D P, WANG Y Y, et al.Lactobacillus casei protects intestinal mucosa from damage in chicks caused by Salmonella pullorum via regulating immunity and the Wnt signaling pathway and maintaining the abundance of gut microbiota[J].Poultry Science, 2021, 100(8):101283.
[22] LIU S D, SONG M H, YUN W, et al.Effect of carvacrol essential oils on immune response and inflammation-related genes expression in broilers challenged by lipopolysaccharide[J].Poultry Science, 2019, 98(5):2026-2033.  
[23] YANG X, LIU Y L, YAN F, et al.Effects of encapsulated organic acids and essential oils on intestinal barrier, microbial count, and bacterial metabolites in broiler chickens[J].Poultry Science, 2019, 98(7):2858-2865.  
[24] PALAMIDI I, MOUNTZOURIS K C.Diet supplementation with an organic acids-based formulation affects gut microbiota and expression of gut barrier genes in broilers[J].Animal Nutrition, 2018, 4(4):367-377.  
[25] YARMOHAMMADI BARBARESTANI S, JAZI V, MOHEBODINI H, et al.Effects of dietary lavender essential oil on growth performance, intestinal function, and antioxidant status of broiler chickens[J].Livestock Science, 2020, 233:103958.
[26] 陈继发, 曲湘勇.蒙脱石和枯草芽孢杆菌对蛋鸡血浆生化指标、小肠刷状缘酶活性及黏蛋白表达的影响[J].中国畜牧杂志, 2020, 56(9):192-197. CHEN J F, QU X Y.Effects of smectite and Bacillus subtilis on plasma biochemical parameters, brush border enzymes activities, and the expressions of mucoproteins in small intestine of laying hens[J].Chinese Journal of Animal Science, 2020, 56(9):192-197.(in Chinese)
[27] LIU S D, SONG M H, YUN W, et al.Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers[J].Journal of Animal Physiology and Animal Nutrition, 2018, 102(5):1257-1265.  
[28] 郭彤, 吴艳, 李晓晨, 等.Cu2+/ZnO-蒙脱石对断奶仔猪生长性能、肠道菌群、黏膜二糖酶活性及肠黏膜形态的影响[J].中国畜牧杂志, 2016, 52(17):48-53, 59. GUO T, WU Y, LI X C, et al.Effects of Cu2+/ZnO- montmorillonite complexes on growth performance, intestinal microflora, mucosa disaccharidase activities and morphology of weanling pigs[J].Chinese Journal of Animal Science, 2016, 52(17):48-53, 59.(in Chinese)
[29] AMBROSIO C M S, DE ALENCAR S M, DE SOUSA R L M, et al.Antimicrobial activity of several essential oils on pathogenic and beneficial bacteria[J].Industrial Crops and Products, 2017, 97:128-136.
[30] QARALLEH H.Thymol rich Thymbra capitata essential oil inhibits quorum sensing, virulence and biofilm formation of beta lactamase producing Pseudomonas aeruginosa[J].Natural Product Sciences, 2019, 25(2):172-180.  
[31] SHAO D Y, LI J, LI J, et al.Inhibition of gallic acid on the growth and biofilm formation of Escherichia coli and Streptococcus mutans[J].Journal of Food Science, 2015, 80(6):M1299-M1305.
文章导航

/