研究论文 RESEARCH PAPER

菌酶复合制剂对育肥湖羊生长性能、瘤胃发酵和细菌菌群的影响

  • 毕梦凡 ,
  • 王平 ,
  • 王利军 ,
  • 刘超齐 ,
  • 左瑞雨 ,
  • 朱湲湲 ,
  • 尹清强 ,
  • 宁长申 ,
  • 常娟
展开
  • 1. 河南农业大学动物科技学院, 郑州 450046;
    2. 河南省畜牧总站, 郑州 450008
毕梦凡(1997—),女,河南洛阳人,硕士研究生,从事饲料生物技术研究。E-mail:bimengfan0114@163.com

收稿日期: 2022-04-21

  网络出版日期: 2022-11-14

基金资助

河南省科技攻关项目(212102110006);河南省重大科技专项(201111311100);河南省教育厅重点科研项目(21A230012)

Effects of Probiotics and Enzyme Compound Preparation on Growth Performance, Rumen Fermentation and Bacterial Community of Fattening Hu Sheep

  • BI Mengfan ,
  • WANG Ping ,
  • WANG Lijun ,
  • LIU Chaoqi ,
  • ZUO Ruiyu ,
  • ZHU Yuanyuan ,
  • YIN Qingqiang ,
  • NING Changshen ,
  • CHANG Juan
Expand
  • 1. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China;
    2 Henan Provincial Animal Husbandry General Station, Zhengzhou 450008, China

Received date: 2022-04-21

  Online published: 2022-11-14

摘要

本试验旨在研究菌酶复合制剂对育肥湖羊生长性能、瘤胃发酵和细菌菌群的影响。试验选取156只60日龄体重相近、健康状况良好的雄性育肥湖羊,随机分为4个组,每组3个重复,每个重复13只羊。对照组(A组)饲喂基础饲粮,试验组饲喂在基础饲粮的基础上分别添加0.1%(B组)、0.2%(C组)和0.3%(D组)菌酶复合制剂的饲粮。试验预试期7 d,正试期60 d。结果表明:1)与A组相比,D组第31~60天及第1~60天湖羊平均日增重(ADG)显著提高(P<0.05),料重比(F/G)显著降低(P<0.05)。2)与A组相比,B组、C组和D组酸性洗涤纤维(ADF)表观消化率显著提高(P<0.05),B组和C组中性洗涤纤维(NDF)表观消化率显著提高(P<0.05)。3) D组瘤胃pH显著低于A组、B组和C组(P<0.05);与A组相比,B组、C组和D组瘤胃氨态氮(NH3-N)含量显著提高(P<0.05),瘤胃总挥发性脂肪酸、丙酸和异丁酸含量显著降低(P<0.05);B组和D组瘤胃丁酸含量显著降低(P<0.05)。4)16S rRNA测序结果表明,与A组相比,在门水平上,D组拟杆菌门(Bacteroidetes)和TM7相对丰度显著降低(P<0.05),放线菌门(Actinobacteria)和蓝藻菌门(Cyanobacteria)相对丰度显著提高(P<0.05);在属水平上,D组欧陆森氏菌属(Olsenella)相对丰度显著提高(P<0.05),YRC22相对丰度显著降低(P<0.05)。5)密螺旋体属(Treponema)相对丰度与NDF表观消化率和瘤胃NH3-N含量呈极显著正相关(P<0.01);欧陆森氏菌属相对丰度与ADF表观消化率呈显著正相关(P<0.05),与瘤胃pH和丙酸含量呈显著负相关(P<0.05)。综上所述,在饲粮中添加0.3%的菌酶复合制剂可以提高育肥湖羊ADG,降低F/G,提高饲粮ADF表观消化率,降低瘤胃拟杆菌门相对丰度,同时提高瘤胃欧陆森氏菌属相对丰度,有助于提高对饲粮中纤维的消化率。

本文引用格式

毕梦凡 , 王平 , 王利军 , 刘超齐 , 左瑞雨 , 朱湲湲 , 尹清强 , 宁长申 , 常娟 . 菌酶复合制剂对育肥湖羊生长性能、瘤胃发酵和细菌菌群的影响[J]. 动物营养学报, 2022 , 34(11) : 7228 -7239 . DOI: 10.3969/j.issn.1006-267x.2022.11.041

Abstract

The purpose of this experiment was to study the effects of probiotics and enzyme compound preparation on the growth performance, rumen fermentation and bacterial community of fattening Hu sheep. A total of 156 healthy 60-day-old male fattening Hu sheep with similar body weight were randomly divided into 4 groups with 3 replicates per group and 13 sheep per replicate. Sheep in the control group (group A) were fed a basal diet, and those in the experimental groups were fed the basal diets supplemented with 0.1% (group B), 0.2% (group C) and 0.3% (group D) probiotics and enzyme compound preparation, respectively. The pre-trial period lasted for 7 days, and the trial period lasted for 60 days. The results showed as follows:1) compared with group A, the average daily gain (ADG) of Hu sheep from days 31 to 60 and 1 to 60 in group D was significantly increased (P<0.05), and the ratio of feed to gain (F/G) was significantly decreased (P<0.05). 2) Compared with group A, the acid detergent fiber (ADF) apparent digestibility in groups B, C and D was significantly increased (P<0.05), and the neutral detergent fiber (NDF) apparent digestibility in groups B and C was significantly increased (P<0.05). 3) The rumen pH in group D was significantly lower than that in groups A, B and C (P<0.05). Compared with group A, the rumen ammonia nitrogen (NH3-N) content in groups B, C and D was significantly increased (P<0.05), and the rumen contents of total volatile fatty acids, propionic acid and isobutyric acid in groups B, C and D were significantly decreased (P<0.05); the rumen butyric acid content in groups B and D was significantly decreased (P<0.05). 4) The 16S rRNA sequencing results showed that compared with group A, at the phylum level, the relative abundances of Bacteroidetes and TM7 in group D were significantly decreased (P<0.05), and the relative abundances of Actinobacteria and Cyanobacteria were significantly increased (P<0.05); at the genus level, the Olsenella relative abundance in group D was significantly increased (P<0.05), and the YRC22 relative abundance was significantly decreased (P<0.05). 5) The Treponema relative abundance was extremely significantly positively correlated with NDF apparent digestibility and rumen NH3-N content (P<0.01). The Olsenella relative abundance was significantly positively correlated with ADF apparent digestibility (P<0.05), and significantly negatively correlated with rumen pH and propionic acid content (P<0.05). In conclusion, dietary 0.3% probiotics and enzyme compound preparation can increase ADG, decrease F/G, increase ADF apparent digestibility, decrease Bacteroidetes relative abundance, and increase Olsenella relative abundance in rumen, which is helpful to improve dietary fiber digestibility of fattening Hu sheep.

参考文献

[1] UYENO Y, SHIGEMORI S G U, SHIMOSATO T.Effect of probiotics/prebiotics on cattle health and productivity[J].Microbes and Environments, 2015, 30(2):126-132.  
[2] 肖君.米曲霉培养物对绵羊瘤胃发酵和菌群多样性的影响[D].硕士学位论文.长春:吉林农业大学, 2015. XIAO J.Effects of Aspergillus oryzae culture on rumen fermentation and microbial diversity[D].Master's Thesis.Changchun:Jilin Agricultural University, 2015.(in Chinese)
[3] MIADOKOVÁ E, SVIDOVÁ S, VLCKOVÁ V, et al.Diverse biomodulatory effects of glucomannan from Candida utilis[J].Toxicology in Vitro, 2006, 20(5):649-657.  
[4] VLČKOVÁ V, DÚHOVÁ V, SVIDOVÁ S, et al.Antigenotoxic potential of glucomannan on four model test systems[J].Cell Biology and Toxicology, 2004, 20(6):325-332.  
[5] 吴爽, 周玉香, 贾柔, 等.纤维素酶处理荞麦秸秆对其纤维结构和滩羊肉品质的影响[J].草业学报, 2021, 30(1):170-180. WU S, ZHOU Y X, JIA R, et al.Effects of cellulase treatment of buckwheat straw on fiber structure and meat quality of Tan sheep[J].Acta Prataculturae Sinica, 2021, 30(1):170-180.(in Chinese)
[6] 张对红.米曲霉培养物对湖羊生产性能和瘤胃功能的影响[D].硕士学位论文.兰州:兰州大学, 2021. ZHANG D H.Effects of Aspergillus oryzae culture on the performance and rumen function of Hu sheep[D].Master's Thesis.Lanzhou:Lanzhou University, 2021.(in Chinese)
[7] ZERBY H N, BARD J L, LOERCH S C, et al.Effects of diet and Aspergillus oryzae extract or Saccharomyces cervisiae on growth and carcass characteristics of lambs and steers fed to meet requirements of natural markets[J].Journal of Animal Science, 2011, 89(7):2257-2264.  
[8] 丁洪涛, 刘星, 夏冬华, 等.产朊假丝酵母对奶牛体外瘤胃发酵参数及日粮营养物质消化率的影响[J].中国畜牧杂志, 2012, 48(9):56-59. DING H T, LIU X, XIA D H, et al.Effects of Candida utilis on simulated rumen fermentation parameters and dietary nutrient digestibility of dairy cows[J].Chinese Journal of Animal Science, 2012, 48(9):56-59.(in Chinese)
[9] 肖杰, 孙攀峰, 李鹏伟, 等.饲粮中添加单宁和纤维素酶对育肥湖羊生长性能、营养物质消化率及肠道形态的影响[J].中国饲料, 2020(8):33-36. XIAO J, SUN P F, LI P W, et al.Effects of tannins and cellulase on growth performance, nutrients digestibility and intestinal morphology of Hu sheep[J].China Feed, 2020(8):33-36.(in Chinese)
[10] 姜碧薇, 周玉香, 王甜, 等.酶菌混合处理稻草和苜蓿干草对滩羊生长性能、血清生化指标、瘤胃细菌多样性及KEGG通路的影响[J].动物营养学报, 2021, 33(3):1482-1492. JIANG B W, ZHOU Y X, WANG T, et al.Effects of straw and alfalfa hay treated by enzyme and bacteria on growth performance, serum biochemical indexes, rumen bacterial diversity and KEGG pathway of Tan sheep[J].Chinese Journal of Animal Nutrition, 2021, 33(3):1482-1492.(in Chinese)
[11] VAN SOEST P J, ROBERTSON J B, LEWIS B A.Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science, 1991, 74(10):3583-3597.  
[12] BRODERICK G A, KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science, 1980, 63(1):64-75.  
[13] 牟豪杰, 孟庆翔, 任丽萍, 等.离子色谱法同时测定牛瘤胃液中乳酸和挥发性脂肪酸含量[J].理化检验(化学分册), 2009, 45(1):52-54. MOU H J, MENG Q X, REN L P, et al.Simultaneous determination of lactic acid and volatile fatty acids in ox rumen juice by ion chromatography[J].Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2009, 45(1):52-54.(in Chinese)
[14] ANGELAKIS E.Weight gain by gut microbiota manipulation in productive animals[J].Microbial Pathogenesis, 2017, 106:162-170.
[15] KRITAS S K, GOVARIS A, CHRISTODOULOPOULOS G, et al.Effect of Bacillus licheniformis and Bacillus subtilis supplementation of ewe's feed on sheep milk production and young lamb mortality[J].Journal of Veterinary Medicine A, 2006, 53(4):170-173.  
[16] 邱玉朗, 李林, 纪传来, 等.秸秆玉米浆混合发酵对肉羊生长及血液指标的影响[J].饲料研究, 2019, 42(1):12-14. QIU Y L, LI L, JI C L, et al.Effects of straw corn slurry mixed fermentation on growth and blood indexes of mutton sheep[J].Feed Research, 2019, 42(1):12-14.(in Chinese)
[17] HERNÁNDEZ R, GONZÁLEZ S S, PINOS-RODRÍGUEZ J M, et al.Effect of a yeast culture on nitrogen balance and digestion in lambs fed early and mature orchard grass[J].Journal of Applied Animal Research, 2009, 35(1):53-56.  
[18] 李忠玲, 杜忍让, 武和平, 等.复合酶制剂对陕北绒山羊增重效果的研究[J].畜牧兽医杂志, 2014, 33(6):4-7. LI Z L, DU R R, WU H P, et al.Study on gaining effect of compound enzyme preparation in Shanbei white-cashmere goat[J].Journal of Animal Science and Veterinary Medicine, 2014, 33(6):4-7.(in Chinese)
[19] 拉加.多酶益生素处理青稞秸秆饲喂育肥羊增重效果试验[J].山东畜牧兽医, 2018, 39(3):7-8. LA J.Effect of multi enzyme probiotics on weight gain of fattening sheep fed with highland barley straw[J].Shandong Journal of Animal Science and Veterinary Medicine, 2018, 39(3):7-8.(in Chinese)
[20] 肖怡.三种益生菌对肉羊甲烷排放、物质代谢和瘤胃发酵的影响[D].硕士学位论文.阿拉尔:塔里木大学, 2016. XIAO Y.Effects of three probiotics on methane emission, nutrient metabolism and rumen fermentation in mutton sheep[D].Master's Thesis.Alar:Tarim University, 2016.(in Chinese)
[21] NEWBOLD C J, MCINTOSH F M, WALLACE R J.Changes in the microbial population of a rumen-simulating fermenter in response to yeast culture[J].Canadian Journal of Animal Science, 1998, 78(2):241-244.  
[22] 王红梅, 屠焰, 司丙文, 等.不同配伍酶制剂处理玉米秸秆对肉用绵羊生长性能和营养物质消化率的影响[J].中国农业科学, 2016, 49(24):4806-4813. WANG H M, TU Y, SI B W, et al.Effects of corn straw treated with different compatibility enzymes on growth performance and nutrient digestibility of mutton sheep[J].Scientia Agricultura Sinica, 2016, 49(24):4806-4813.(in Chinese)
[23] 曾辉, 邱玉朗, 李林, 等.酶制剂和乳酸菌对秸秆微贮饲料质量及瘤胃降解率的影响[J].中国畜牧杂志, 2018, 54(11):84-88. ZENG H, QIU Y L, LI L, et al.Effect of enzyme preparation and Lactobacillus on the quality and rumen degradation rate of corn stalks microbial feeds[J].Chinese Journal of Animal Science, 2018, 54(11):84-88.(in Chinese)
[24] WANG Y, MCALLISTER T A, RODE L M, et al.Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the rumen simulation technique (Rusitec)[J].The British Journal of Nutrition, 2001, 85(3):325-332.  
[25] 李孟伟, 彭丽娟, 彭开屏, 等.抑制不同种类瘤胃微生物活性对水牛瘤胃发酵和脂肪酸代谢的影响[J].中国畜牧兽医, 2020, 47(9):2767-2778. LI M W, PENG L J, PENG K B, et al.Effects of inhibition of different rumen microbial activity on rumen fermentation and fatty acid metabolism in buffaloes[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(9):2767-2778.(in Chinese)
[26] WALLACE R J, ARTHAUD L, NEWBOLD C J.Influence of Yucca shidigera extract on ruminal ammonia concentrations and ruminal microorganisms[J].Applied and Environmental Microbiology, 1994, 60(6):1762-1767.  
[27] MATTHEWS C, CRISPIE F, LEWIS E, et al.The rumen microbiome:a crucial consideration when optimising milk and meat production and nitrogen utilisation efficiency[J].Gut Microbes, 2019, 10(2):115-132.  
[28] 苏丽萍, 何长芳, 马双青, 等.添加复合纤维素酶对青海育肥藏系羔羊瘤胃发酵的影响研究[J].青海畜牧兽医杂志, 2014, 44(4):22-24. SU L P, HE C F, MA S Q, et al.Effect of adding compound cellulase enzyme on rumen fermentation of fattening lamb[J].Chinese Qighai Journal of Animal and Veterinary Sciences, 2014, 44(4):22-24.(in Chinese)
[29] 牛建康.活性干酵母对泌乳牛生产性能、瘤胃发酵及甲烷排放的影响[D].硕士学位论文.保定:河北农业大学, 2019. NIU J K.Effects of active dry yeasts on performance, rumen fermentation and methane production of lactating dairy cows[D].Master's Thesis.Baoding:Agricultural University of Hebei, 2019.(in Chinese)
[30] COLLADO M C, ISOLAURI E, SALMINEN S, et al.The impact of probiotic on gut health[J].Current Drug Metabolism, 2009, 10(1):68-78.  
[31] LEE J E, LEE S, SUNG J, et al.Analysis of human and animal fecal microbiota for microbial source tracking[J].The ISME Journal, 2011, 5:362-365.
[32] CHANG J, WANG T, WANG P, et al.Compound probiotics alleviating aflatoxin B1 and zearalenone toxic effects on broiler production performance and gut microbiota[J].Ecotoxicology and Environmental Safety, 2020, 194:110420.
[33] SUL'ÁK M, SIKOROVÁ L, JANKUVOVÁ J, et al.Variability of actinobacteria, a minor component of rumen microflora[J].Folia Microbiologica, 2012, 57(4):351-353.  
[34] PANDYA P R, SINGH K M, PARNERKAR S, et al.Bacterial diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16S rDNA analysis[J].Journal of Applied Genetics, 2010, 51(3):395-402.  
[35] 钟港, 陈东, 田科雄, 等.发酵菌糠替代白酒糟对育肥牛瘤胃发酵参数、微生物菌群结构和功能的影响[J].动物营养学报, 2022, 34(1):381-394. ZHONG G, CHEN D, TIAN K X, et al.Effects of replacing distiller's grains with fermented spent mushroom substrate on rumen fermentation parameters, microbial community structure and function of fattening cattle[J].Chinese Journal of Animal Nutrition, 2022, 34(1):381-394.(in Chinese)
[36] ZHAO L P, MENG Q X, REN L P, et al.Effects of nitrate addition on rumen fermentation, bacterial biodiversity and abundance[J].Asian-Australasian Journal of Animal Sciences, 2015, 28(10):1433-1441.  
[37] 毛丙永.功能性低聚糖对肠道细菌的影响及机制[D].博士学位论文.无锡:江南大学, 2015. MAO B Y.Effects of functional oligosaccharides on the gut bacteria and the mechanism[D].Ph.D. Thesis.Wuxi:Jiangnan University, 2015.(in Chinese)
[38] MAGER L F, BURKHARD R, PETT N, et al.Microbiome-derived inosine modulates response to checkpoint inhibitor immunotherapy[J].Science, 2020, 369(6510):1481-1489.  
[39] GAOWA N R, PANKE-BUISSE K, WANG S X, et al.Brisket disease is associated with lower volatile fatty acid production and altered rumen microbiome in Holstein heifers[J].Animals, 2020, 10(9):1712.
[40] 李希, 毛杨毅, 罗惠娣, 等.饲粮纤维水平对育肥羔羊瘤胃微生物组成及多样性的影响[J].中国畜牧兽医, 2021, 48(4):1251-1263. LI X, MAO Y Y, LUO H D, et al.Effects of dietary crude fiber level on rumen microbial composition and diversity of fattening lambs[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(4):1251-1263.(in Chinese)
文章导航

/