动物营养学报 >

2022 , Vol. 34 >Issue 11: 7361 - 7369

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.11.053

研究论文 RESEARCH PAPER

不同浓度短链脂肪酸对奶牛瘤胃上皮细胞免疫反应、紧密连接蛋白和G-蛋白偶联受体41表达的影响

  • 詹康 ,
  • 谭德金 ,
  • 孟梓橦 ,
  • 马晓宇 ,
  • 赵国琦
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  • 扬州大学动物科学与技术学院, 扬州 225009
詹康(1988—),男,江苏南京人,讲师,博士,主要从事动物营养与饲料研究。E-mail:kzhan@yzu.edu.cn

收稿日期: 2022-05-05

  网络出版日期: 2022-11-14

基金资助

江苏省自然科学基金项目（BK20190898）；国家自然科学基金项目（32002200）；财政部和农业农村部国家现代农业产业技术体系资助项目（CARS-36）

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Effects of Different Concentrations of Short-Chain Fatty Acids on Immune Response, Tight Junction Protein and G Protein-Coupled Receptor 41 Expression in Bovine Rumen Epithelial Cells

  • ZHAN Kang ,
  • TAN Dejin ,
  • MENG Zitong ,
  • MA Xiaoyu ,
  • ZHAO Guoqi
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2022-05-05

  Online published: 2022-11-14

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摘要

本试验旨在研究不同浓度短链脂肪酸（SCFAs）对奶牛瘤胃上皮细胞（BRECs）免疫反应、紧密连接蛋白和G-蛋白偶联受体41（GPR41）表达的影响以及GPR41与促炎症因子、趋化因子和紧密连接蛋白之间的关系。试验设计4个试验组，分别用20、50、100和150 mmol/L SCFAs培养BRECs 24 h后提取总RNA，每组3个重复。结果显示：1） SCFAs浓度为100和150 mmol/L SCFAs时，促炎症因子肿瘤坏死因子-α（TNF-α）的mRNA相对表达量显著高于SCFAs浓度为20和50 mmol/L时（P<0.05）。2）随着SCFAs浓度的增加，CXC趋化因子配体3（CXCL3）、CXC趋化因子配体5（CXCL5）和CXC趋化因子配体8（CXCL8）的mRNA相对表达量逐渐下调，以SCFAs浓度为150 mmol/L时最低。SCFAs浓度为100 mmol/L时，GPR41的mRNA相对表达量显著高于SCFAs浓度为20和50 mmol/L时（P<0.05）。3） SCFAs浓度为150 mmol/L时紧密连接蛋白闭合蛋白-1（Claudin-1）和闭锁小带蛋白-1（ZO-1）的mRNA相对表达量最低，且显著低于SCFAs浓度为20和50 mmol/L时（P<0.05）。4）相关性分析表明，GPR41的mRNA相对表达量与促炎症因子TNF-α的mRNA相对表达量呈显著正相关（P<0.05），与趋化因子CXCL3、CXCL5和CXCL8的mRNA相对表达量呈显著负相关（P<0.05），与紧密连接蛋白Claudin-1和ZO-1的mRNA相对表达量呈负相关趋势（0.05≤P<0.10）。综上所述，高浓度（100和150 mmol/L） SCFAs诱导BRECs的促炎症反应，抑制趋化因子表达和免疫反应，减少紧密连接蛋白的表达，破坏瘤胃上皮的屏障功能。

关键词： 奶牛瘤胃上皮细胞; 短链脂肪酸; 免疫; 趋化因子; G-蛋白偶联受体41

本文引用格式

詹康 , 谭德金 , 孟梓橦 , 马晓宇 , 赵国琦 . 不同浓度短链脂肪酸对奶牛瘤胃上皮细胞免疫反应、紧密连接蛋白和G-蛋白偶联受体41表达的影响[J]. 动物营养学报, 2022 , 34(11) : 7361 -7369 . DOI: 10.3969/j.issn.1006-267x.2022.11.053

Abstract

To investigate the effects of different concentrations of short-chain fatty acids (SCFAs) on immune response, tight junction protein and G protein-coupled receptor 41 (GPR41) expression in bovine rumen epithelial cells (BRECs) and the relationships between GPR41 and pro-inflammatory factors, chemokines and tight junction proteins. Four experimental groups were designed with three replicates each. BRECs of the four experimental groups were cultured with 20, 50, 100 and 150 mmol/L SCFAs, and cellular total RNA were extracted after 24 h of culture. The results showed as follows:1) the mRNA expression level of pro-inflammatory factor tumor necrosis factor-α (TNF-α) at 100 and 150 mmol/L SCFAs was significantly higher than that at 20 and 50 mmol/L SCFAs (P<0.05). 2) The mRNA expression levels of CXC chemokine ligand 3 (CXCL3), CXC chemokine ligand 5 (CXCL5) and CXC chemokine ligand 8 (CXCL8) were gradually downregulated with the increase of SCFAs concentration, and they were lowest when BRECs incubated with 150 mmol/L SCFAs. The mRNA expression levels of GPR41 at the concentration of 100 mmol/L SCFAs was significantly higher than that at the concentrations of 20 and 50 mmol/L SCFAs (P<0.05). 3) When the concentration of SCFAs was 150 mmol/L, it had the lowest mRNA expression levels of tight junction proteins claudin-1 (Claudin-1) and zonula occludens-1 (ZO-1), which were significantly decreased compared with 20 and 50 mmol/L SCFAs (P<0.05). 4) Correlation analysis showed that GPR41 mRNA expression level showed a significant positive correlation with the pro-inflammatory factor TNF-α mRNA expression level (P<0.05). However, a significant negative correlation with the chemokines CXCL3, CXCL5 and CXCL8 mRNA expression levels (P<0.05), and a negative correlation trend with the tight junction proteins Claudin-1 and ZO-1 mRNA expression levels (0.05 ≤ P<0.10). In conclusion, high concentrations (100 and 150 mmol/L) of SCFAs can induce pro-inflammatory responses of BRECs, and inhibit chemokine expression and immune response, reduce the expression of tight junction proteins, and disrupt the barrier function of rumen epithelium.

Key words： bovine rumen epithelial cells; short-chain fatty acids; immune; chemokines; GPR41

参考文献

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