动物营养学报 >

2022 , Vol. 34 >Issue 11: 7402 - 7410

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.11.057

研究论文 RESEARCH PAPER

紫云英苷通过增强核转录因子E2相关因子2基因表达修复力竭运动造成的小鼠肝脏损伤

  • 张文天 ,
  • 吴秋雪 ,
  • 祝锴烨 ,
  • 罗海静 ,
  • 张珍珠 ,
  • 邵淑丽 ,
  • 张伟伟
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  • 1. 齐齐哈尔大学生命科学与农林学院, 齐齐哈尔 161006;
    2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 齐齐哈尔 161006
张文天(1998—),男,黑龙江林甸人,硕士研究生,从事分子生物学研究。E-mail:13359603304@163.com

收稿日期: 2022-04-20

  网络出版日期: 2022-11-14

基金资助

黑龙江省自然科学基金项目（LH2021C099）；黑龙江省教育厅基本业务专项（135109255）；黑龙江省省属高等学校基本科研业务费科研项目（植物性食品加工技术特色学科专项）（YSTSXK201890）；齐齐哈尔大学研究生创新科研项目（YJSCX2021032）

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Astragalin Enhances Nuclear Factor E2 Related Factor 2 Gene Expression to Repair Liver Injury Induced by Exhaustive Exercise in Mice

  • ZHANG Wentian ,
  • WU Qiuxue ,
  • ZHU Kaiye ,
  • LUO Haijing ,
  • ZHANG Zhenzhu ,
  • SHAO Shuli ,
  • ZHANG Weiwei
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  • 1. School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;
    2. Key Laboratory of Resistant Genetic Engineering and Cold Biodiversity Conservation of Heilongjiang Province, Qiqihar 161006, China

Received date: 2022-04-20

  Online published: 2022-11-14

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摘要

为探索紫云英苷对力竭运动小鼠肝脏损伤的修复作用，选取48只8周龄无特异性病原体（SPF）级C57BL/6J雄性小鼠，随机分成4组，分别为二甲基亚砜（DMSO）组、力竭运动组、50 mg/kg紫云英苷组、100 mg/kg紫云英苷组，其中DMSO组不做运动，其余3组进行一次性力竭运动4 h。2个紫云英苷组在力竭运动后分别灌胃50和100 mg/kg BW紫云英苷，力竭运动组在力竭运动后灌胃等量生理盐水，DMSO组灌胃等量生理盐水配制的0.1% DMSO。灌胃1 h后处死小鼠，检测小鼠肝脏中羟自由基（·OH）、超氧阴离子（O2-）清除能力，谷胱甘肽（GSH）含量，谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GSH-Px）活性以及核转录因子E2相关因子2（Nrf2）、谷氨酸-半胱氨酸连接酶催化亚基（GCLC）和谷氨酸-半胱氨酸连接酶调节亚基（GCLM）的基因和蛋白表达情况。结果显示：与DMSO组相比，力竭运动组小鼠肝脏中羟自由基（·OH）和超氧阴离子（O2-）清除能力分别降低54%（P<0.01）和43%（P<0.01），GSH含量降低27%（P<0.05），GR和GSH-Px活性分别降低32%（P<0.01）和37%（P<0.05），同时Nrf2和GCLM的mRNA和蛋白相对表达量显著或极显著降低（P<0.05或P<0.01）。与力竭运动组相比，灌胃浓度为50、100 mg/kg的紫云英苷分别使小鼠肝脏中·OH清除能力提高1.45（P<0.05）和1.25倍（P<0.01），O2-清除能力提高1.78（P<0.01）和1.95倍（P<0.01），GSH含量提高1.36（P<0.05）和2.21倍（P<0.01），GSH-Px活性提高1.54（P<0.01）和1.99倍（P<0.01），GR活性提高1.42（P<0.01）和1.62倍（P<0.01）。此外，与力竭运动组相比，灌胃浓度为50和100 mg/kg的紫云英苷均可使小鼠肝脏中Nrf2、GCLC、GCLM的mRNA和蛋白相对表达量显著或极显著升高（P<0.05或P<0.01）。综上可知，适量的紫云英苷可通过上调Nrf2以及GCLC、GCLM基因的表达修复力竭运动对小鼠肝脏造成的损伤。

关键词： 紫云英苷; 力竭运动; 肝脏损伤

本文引用格式

张文天 , 吴秋雪 , 祝锴烨 , 罗海静 , 张珍珠 , 邵淑丽 , 张伟伟 . 紫云英苷通过增强核转录因子E2相关因子2基因表达修复力竭运动造成的小鼠肝脏损伤[J]. 动物营养学报, 2022 , 34(11) : 7402 -7410 . DOI: 10.3969/j.issn.1006-267x.2022.11.057

Abstract

To explore the repairing effect of astragalin on liver injury in exhaustive exercise mice, forty-eight 8-week-old specific-pathogen-free (SPF) grade C57BL/6J male mice were randomly divided into four groups:DMSO group, exhaustion exercise group, 50 mg/kg astragalin group and 100 mg/kg astragalin group. In addition to the DMSO group, the other three groups received acute exhaustive exercise for 4 h. After exhaustive exercise, the mice of the two astragalin groups were gavaged with 50 and 100 mg/kg BW astragalin, respectively, the mice of the exhaustion exercise group were gavaged with the same amount of normal saline, and the mice of the DMSO group were gavaged with 0.1% DMSO prepared in normal saline. The mice were killed one hour later, and the scavenging capacity of hydroxyl radical superoxide anion, glutathione content, the activities of glutathione reductase and glutathione peroxidase, and the proteins and genes expression of Nrf2, glutamate-cysteine ligase cysteine subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) in liver of mice were determined. The results show that, compared with the DMSO group, after acute exhaustive exercise the scavenging capacity of ·OH and O2- in mice liver decreased by 54% (P<0.01) and 43% (P<0.01), respectively, the content of GSH decreased by 27% (P<0.05), the activities of GR and GSH-Px decreased by 32% (P<0.01) and 37% (P<0.05), respectively, and the mRNA and protein relative expression levels of Nrf2 and GCLM were significantly or extremely significantly decreased (P<0.05 or P<0.01). Compared with the exhaustive exercise group, the scavenging ability of ·OH in 50 and 100 mg/kg astragalin groups increased by 1.45 (P<0.01) and 1.25 times (P<0.01), respectively, the scavenging capacity of O2- increased by 1.78 (P<0.01) and 1.95 times (P<0.01), the content of GSH increased by 1.36 (P<0.05) and 2.21 times (P<0.01), the activity of GSH-Px increased by 1.54 (P<0.01) and 1.99 times (P<0.01), the activity of GR increased by 1.42 (P<0.01) and 1.62 times (P<0.01). Moreover, the mRNA and protein relative expression levels of Nrf2, GCLC and GCLM in liver of mice were significantly or extremely significantly increased compared with the exhaustive exercise group (P<0.05 or P<0.01). In conclusion, appropriate amount of astragalin can repair the injury of mouse liver caused by exhaustive exercise through up-regulating Nrf2, GCLC and GCLM genes expression.

Key words： astragalin; exhaustive exercise; liver injury

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