1 材料与方法
1.1 试验设计
表1 试验饲粮组成及营养水平(干物质基础)Table 1 Composition and nutrient levels of experimental diets (DM basis)% |
| 项目 Items | 1组 Group 1 | 2组 Group 2 | 3组 Group 3 | 4组 Group 4 | 5组 Group 5 |
|---|---|---|---|---|---|
| 原料 Ingredients | |||||
| 玉米 Corn | 29.0 | 25.0 | 25.0 | 18.0 | 15.0 |
| 玉米淀粉 Corn starch | 7.0 | 6.5 | 3.5 | 4.0 | 3.0 |
| 麦麸 Wheat bran | 1.0 | 4.0 | 2.5 | 5.5 | 5.4 |
| 豆粕 Soybean meal | 4.0 | 9.8 | 13.3 | 18.5 | |
| 菜籽粕 Rapeseed meal | 1.7 | 1.5 | 2.5 | 3.6 | |
| 全株玉米青贮 Whole plant corn silage | 57.6 | 52.0 | 50.0 | 49.0 | 46.0 |
| 小麦秸 Wheat straw | 2.0 | 3.5 | 4.5 | 4.5 | 5.4 |
| 磷酸氢钙 CaHPO4 | 1.9 | 1.8 | 1.7 | 1.7 | 1.6 |
| 预混料 Premix1) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| 食盐 NaCl | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| 合计 Total | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| 营养水平 Nutrient levels2) | |||||
| 粗蛋白质 CP | 8.54 | 10.59 | 12.56 | 14.51 | 16.50 |
| 钙 Ca | 0.69 | 0.69 | 0.68 | 0.68 | 0.67 |
| 磷 P | 0.39 | 0.39 | 0.38 | 0.37 | 0.36 |
| 中性洗涤纤维 NDF | 34.73 | 34.50 | 34.27 | 34.85 | 34.81 |
| 酸性洗涤纤维 ADF | 18.97 | 18.61 | 18.71 | 18.94 | 19.08 |
| 代谢能 ME/(MJ/kg) | 9.17 | 9.09 | 9.10 | 9.09 | 9.09 |
1)预混料为每千克饲粮提供 The premix provided the following per kg of diets: VA 10 000 IU,VD 1 000 IU,VE 50 IU,Fe (as ferrous sulfate) 140 mg,Zn (as zinc sulfate) 80 mg,Cu (as copper sulfate) 10 mg,Mn (as manganese sulfate) 58 mg,I (as potassium iodide) 2.5 mg,Se (as sodium selenite) 0.35 mg,Co (as cobalt sulfate) 0.65 mg。 2)代谢能为计算值,其余营养水平为实测值。ME was a calculated value, while other nutrient levels were measured values. |
1.2 饲养管理
1.3 消化代谢试验
1.4 指标测定方法及公式
1.5 数据处理
2 结果与分析
2.1 饲粮CP水平对生长期云上黑山羊生长性能的影响
表2 饲粮CP水平对生长期云上黑山羊生长性能的影响Table 2 Effects of dietary CP level on growth performance of Yunshang black goats during growth period |
| 项目 Items | 1组 Group 1 | 2组 Group 2 | 3组 Group 3 | 4组 Group 4 | 5组 Group 5 |
|---|---|---|---|---|---|
| 初始体重 IBM/kg | 28.15±1.19 | 28.12±1.04 | 28.21±1.08 | 28.11±1.21 | 28.18±1.14 |
| 终末体重 FBW/kg | 30.56±1.17 | 31.36±1.25 | 32.36±1.72 | 31.77±1.26 | 31.50±1.29 |
| 平均日增重 ADG/(g/d) | 80.38±4.87d | 108.08±4.85c | 135.00±5.25a | 121.59±5.04b | 110.77±3.28c |
| 干物质采食量 DMI/(g/d) | 776.56±28.07c | 826.50±21.16b | 868.33±24.10a | 864.51±35.19a | 864.59±33.61a |
| 料重比 F/G | 10.80±3.12a | 7.75±3.23c | 6.41±1.10e | 7.17±1.23d | 8.04±1.92b |
同行数据肩标不同小写字母表示差异显著(P<0.05),相同或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different small letter superscripts mean significant difference (P<0.05), while with the same or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.2 饲粮CP水平对生长期云上黑山羊蛋白质代谢的影响
2.3 生长期云上黑山羊维持净蛋白质(NP)需要量
表3 饲粮CP水平对生长期云上黑山羊蛋白质代谢的影响Table 3 Effects of dietary CP level on protein metabolism of Yunshang black goats during growth period |
| 项目 Items | 1组 Group 1 | 2组 Group 2 | 3组 Group 3 | 4组 Group 4 | 5组 Group 5 |
|---|---|---|---|---|---|
| 粗蛋白质摄入量 CPI/(g/d) | 67.98±2.34e | 83.64±2.45d | 108.04±2.67c | 124.50±3.24b | 144.51±2.98a |
| 粪中粗蛋白质含量 Fecal CP content/(g/d) | 41.64±4.35 | 43.36±3.34 | 50.79±6.61 | 46.38±3.27 | 42.27±3.01 |
| 尿中粗蛋白质含量 Urinary CP content/(g/d) | 13.15±1.20e | 19.14±1.32d | 24.54±1.22c | 33.91±2.05b | 49.00±2.11a |
| 可消化粗蛋白质摄入量 DCPI/(g/d) | 33.70±1.35e | 42.38±1.25d | 57.94±0.98c | 77.88±0.89b | 102.50±1.54a |
| 粗蛋白质表观消化率 CP apparent digestibility/% | 40.83±3.26d | 50.61±3.54c | 53.63±3.68c | 62.62±2.21b | 70.89±2.64a |
| 粗蛋白质沉积量 CP deposition/(g/d) | 13.24±1.58e | 21.23±1.24d | 34.31±2.14c | 44.43±2.01b | 53.23±1.98a |

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