1 材料与方法
1.1 试验材料
1.2 试验动物及饲粮
表1 基础饲粮组成及营养水平 (干物质基础)Table 1 Composition and nutrient levels of the basal diet (DM basis)% |
| 项目 Items | 含量 Content |
|---|---|
| 原料 Ingredient | |
| 白酒糟 Distiller’s grains | 30.00 |
| 玉米秸秆青贮 Corn straw silage | 52.00 |
| 花生藤 Peanut straw | 7.00 |
| 万寿菊粕 Marigold meal | 10.00 |
| 食盐 NaCl | 0.35 |
| 预混料 Premix1) | 0.65 |
| 合计 Total | 100.00 |
| 营养水平 Nutrient levels2) | |
| 粗蛋白质 CP | 13.22 |
| 中性洗涤纤维 NDF | 52.26 |
| 酸性洗涤纤维 ADF | 33.98 |
| 总能 GE/(MJ/kg) | 15.48 |
1)预混料为每千克饲粮提供The premix provided the following per kg of the diet:VE 3 000 IU,VD3 150 000 IU,VA 500 000 IU,Cu (as copper sulfate) 80 mg,Fe (as ferrous sulfate) 250 mg,Mn (as manganese sulfate) 200 mg,I (as potassium iodide) 1 mg,Zn (as zinc sulfate) 150 mg,Co (as cobalt sulfate) 2 mg,Se (as sodium selenite) 0.5 mg。 2)营养水平均为测定值。Nutrient levels were all measured values. |
1.3 常规营养成分测定
1.4 尼龙袋试验方法
1.5 计算公式
P=a+b(1-e-ct);
ED=a+b×c/(k+c)。
1.6 数据统计
2 结果与分析
2.1 粗饲料常规营养成分含量
表2 3种粗饲料的常规营养成分含量(风干基础)Table 2 Conventional nutritional composition contents in three roughages (air-dry basis)% |
| 项目 Items | 台湾甜象草 Taiwan sweet elephant grass | 桂牧1号杂交象草 Guimu No.1 hybrid elephant grass | 小麦秸秆 Wheat straw | P值 P-value |
|---|---|---|---|---|
| 干物质 DM | 93.20±0.04 | 92.85±0.14 | 95.87±0.05 | 0.250 |
| 粗蛋白质 CP | 7.67±0.07b | 11.41±0.13a | 4.37±1.25c | 0.005 |
| 粗灰分 Ash | 8.03±0.19c | 12.00±0.07b | 13.27±0.25a | <0.001 |
| 中性洗涤纤维 NDF | 65.20±0.66 | 63.97±0.19 | 66.73±1.29 | 0.103 |
| 酸性洗涤纤维 ADF | 37.15±0.16c | 40.37±0.06b | 40.96±0.17a | <0.001 |
同行数据肩标无字母或相同字母表示差异不显著(P>0.05),不同小写字母表示差异显著(P<0.05)。下表同 | |
In the same row, values with no letter or the same letter superscripts mean no significant difference (P>0.05), while with different small letter superscripts mean significant difference (P<0.05). The same as below. |
2.2 粗饲料的营养物质瘤胃降解率
2.2.1 台湾甜象草、桂牧1号杂交象草与小麦秸秆3种粗饲料的DM瘤胃降解率
表3 3种粗饲料的DM瘤胃降解率及降解参数Table 3 Rumen degradability and degradation parameters of DM in three roughages |
| 项目 Items | 台湾甜象草 Taiwan sweet elephant grass | 桂牧1号杂交象草 Guimu No.1 hybrid elephant grass | 小麦秸秆 Wheat straw | P值 P-value |
|---|---|---|---|---|
| 瘤胃降解率 Rumen degradability/% | ||||
| 2 h | 29.98±5.37a | 23.41±3.31b | 13.96±4.20c | <0.001 |
| 4 h | 34.07±2.65a | 31.50±4.55a | 18.36±1.58b | <0.001 |
| 6 h | 34.21±2.70a | 31.91±4.31a | 19.09±1.44b | <0.001 |
| 12 h | 41.28±5.94a | 34.16±6.61b | 19.33±7.13c | <0.001 |
| 24 h | 57.14±2.43a | 53.91±2.37b | 45.36±2.01c | <0.001 |
| 48 h | 65.87±1.31a | 63.69±1.36b | 55.52±1.93c | <0.001 |
| 72 h | 69.17±0.65a | 66.32±1.66b | 60.70±1.44c | <0.001 |
| a/% | 18.66±0.56a | 17.20±0.85a | 8.14±0.51b | <0.001 |
| b/% | 53.91±0.03 | 52.14±1.02 | 57.05±3.79 | 0.084 |
| c/(%/h) | 0.039±0.008 | 0.039±0.003 | 0.037±0.007 | 0.921 |
| ED/% | 48.62±2.48a | 46.12±0.73a | 38.95±1.17b | 0.004 |
a:快速降解部分;b:慢速降解部分;c:慢速降解部分的降解速率;ED:有效降解率。下表同 | |
a: the fraction of immediately degraded; b: the fraction of slowly degraded; c: the degradation rate of the fraction of slowly degraded; ED: the effective degradation. The same as below. |
2.2.2 台湾甜象草、桂牧1号杂交象草与小麦秸秆3种粗饲料的CP瘤胃降解率
表4 3种粗饲料的CP瘤胃降解率及降解参数Table 4 Rumen degradability and degradation parameters of CP in three roughages |
| 项目 Items | 台湾甜象草 Taiwan sweet elephant grass | 桂牧1号杂交象草 Guimu No.1 hybrid elephant grass | 小麦秸秆 Wheat straw | P值 P-value |
|---|---|---|---|---|
| 瘤胃降解率 Rumen degradability/% | ||||
| 2 h | 44.25±6.66 | 38.12±9.31 | 35.69±4.56 | 0.476 |
| 4 h | 44.32±4.63 | 44.82±4.67 | 45.37±8.42 | 0.979 |
| 6 h | 46.87±4.15 | 51.68±4.19 | 45.54±4.17 | 0.266 |
| 12 h | 47.89±7.06 | 55.05±3.38 | 50.04±2.77 | 0.250 |
| 24 h | 62.94±1.21a | 71.05±0.63a | 51.37±6.42b | 0.010 |
| 48 h | 72.69±1.04a | 76.44±0.61a | 52.70±4.05b | <0.001 |
| 72 h | 75.25±1.78a | 82.47±9.78a | 57.90±5.30b | 0.009 |
| 瘤胃降解参数 Rumen degradation parameters | ||||
| a/% | 29.85±0.71a | 25.83±0.71b | 17.29±0.06c | <0.001 |
| b/% | 44.70±0.69a | 42.74±0.71ab | 40.72±0.69b | 0.024 |
| c/(%/h) | 0.049±0.003 | 0.042±0.001 | 0.036±0.005 | 0.063 |
| ED/% | 57.23±1.75a | 50.42±1.47b | 38.95±1.11c | 0.003 |
2.2.3 台湾甜象草、桂牧1号杂交象草与小麦秸秆3种粗饲料的NDF瘤胃降解率
表5 3种粗饲料的NDF瘤胃降解率及降解参数Table 5 Rumen degradability and degradation paramerers of DNF in three roughages |
| 项目 Items | 台湾甜象草 Taiwan sweet elephant grass | 桂牧1号杂交象草 Guimu No.1 hybrid elephant grass | 小麦秸秆 Wheat straw | P值 P-value |
|---|---|---|---|---|
| 瘤胃降解率 Rumen degradability/% | ||||
| 2 h | 15.67±6.48a | 6.89±2.02b | 0.29±0.50c | 0.013 |
| 4 h | 18.75±2.95a | 12.81±0.98b | 0.31±0.53c | <0.001 |
| 6 h | 19.07±1.97a | 16.02±5.01a | 1.44±1.24b | 0.001 |
| 12 h | 29.70±6.53 | 20.99±4.93 | 12.67±6.52 | 0.064 |
| 24 h | 46.83±3.29a | 41.37±2.72a | 33.07±0.40b | 0.007 |
| 48 h | 57.98±1.37a | 54.69±1.02a | 47.84±1.66b | 0.002 |
| 72 h | 63.39±0.22a | 58.73±1.63b | 55.43±2.26c | 0.003 |
| 瘤胃降解参数 Rumen degradation parameters | ||||
| a/% | 6.11±0.56a | 4.00±0.69b | 3.57±0.00b | 0.030 |
| b/% | 58.11±2.15a | 54.56±2.71a | 43.58±0.01b | 0.011 |
| c/(%/h) | 0.051±0.002a | 0.038±0.001b | 0.055±0.003a | 0.007 |
| ED/% | 42.09±0.20a | 33.87±2.43b | 31.43±0.51b | 0.010 |
2.2.4 台湾甜象草、桂牧1号杂交象草与小麦秸秆3种粗饲料的ADF瘤胃降解率
表6 3种粗饲料的ADF瘤胃降解率及降解参数Table 6 Rumen degradability and degradation parameters of ADF in three roughages |
| 项目 Items | 台湾甜象草 Taiwan sweet elephant grass | 桂牧1号杂交象草 Guimu No.1 hybrid elephant grass | 小麦秸秆 Wheat straw | P值 P-value |
|---|---|---|---|---|
| 瘤胃降解率 Rumen degradability/% | ||||
| 2 h | 12.32±6.19a | 3.95±2.17b | 2.09±1.82b | 0.040 |
| 4 h | 13.64±3.13a | 9.58±0.61ab | 3.88±0.33b | 0.022 |
| 6 h | 12.77±0.77a | 10.86±1.63a | 4.50±0.27b | 0.002 |
| 12 h | 26.61±5.53 | 19.20±6.31 | 13.77±1.35 | 0.106 |
| 24 h | 42.89±4.01a | 40.70±3.35a | 28.57±4.71b | 0.010 |
| 48 h | 55.20±1.25a | 48.06±3.41b | 46.83±3.52b | 0.026 |
| 72 h | 60.92±0.56a | 56.73±2.19b | 56.12±1.89b | 0.026 |
| 瘤胃降解参数 Rumen degradation parameters | ||||
| a/% | 1.66±0.34c | 2.75±0.21b | 3.57±0.00a | 0.008 |
| b/% | 61.25±0.33a | 57.85±0.01b | 43.59±0.01c | <0.001 |
| c/(%/h) | 0.046±0.001 | 0.045±0.016 | 0.025±0.002 | 0.165 |
| ED/% | 38.09±0.77a | 36.49±5.15a | 22.79±0.93b | 0.027 |

京公网安备 11010802041926号