1 材料与方法
1.1 伦理声明
1.2 试验材料
1.3 试验设计
1.4 样品收集
1.5 营养成分测定
1.6 发酵品质测定
1.7 相对饲喂价值(RFV)
1.8 瘤胃降解率测定试验
1.8.1 试验设计及饲养管理
表1 基础饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of the basal diet (air-dry basis) % |
| 项目Items | 含量Content |
|---|---|
| 原料Ingredients | |
| 玉米青贮Corn silage | 21.40 |
| 苜蓿干草Alfalfa hay | 11.24 |
| 全棉籽Whole cottonseed | 7.11 |
| 湿啤酒糟Wet brewer’s grains | 4.72 |
| 糖蜜豆皮Molasses bean peel | 6.07 |
| 玉米粉Corn meal | 29.28 |
| 豆粕Soybean meal | 10.89 |
| 菜籽粕Rapeseed meal | 2.18 |
| 玉米蛋白粉Corn gluten meal | 0.70 |
| 脂肪酸钙Fatty acid calcium | 1.69 |
| 石粉Limestone | 0.39 |
| 小苏打NaHCO3 | 0.31 |
| 食盐NaCl | 0.30 |
| 氧化镁MgO | 0.20 |
| 缓释尿素Slow-release urea | 0.49 |
| 葡萄糖Glucose | 1.16 |
| 预混料Premix1) | 1.87 |
| 合计Total | 100.00 |
| 营养水平Nutrient levels2) | |
| 干物质DM | 48.37 |
| 粗蛋白质CP | 16.03 |
| 中性洗涤纤维NDF | 29.06 |
| 酸性洗涤纤维ADF | 17.53 |
| 淀粉Starch | 29.13 |
| 钙Ca | 0.61 |
| 磷P | 0.57 |
| 泌乳净能NEL/(MJ/kg) | 6.93 |
1)每千克预混料含有 One kg of the premix contained the following:VA 9 480 IU,VE 229.2 IU,Cu 1 814.35 mg,Zn 18 760.06 mg,Mn 11 674.90 mg,Se 26.22 mg,Co 143.77 mg,Fe 78.40 mg。 | |
2)泌乳净能为计算值,参照NRC(2001),其余营养水平为实测值。NEL was a calculated value, referring to NRC (2001), while the other nutrient levels were measured values. |
1.8.2 瘤胃降解率测定
1.8.3 计算方法
1.9 奶牛生产性能测定试验
1.9.1 试验设计及饲养管理
表2 试验饲粮组成及营养水平(风干基础)Table 2 Composition and nutrient levels of experimental diets (air-dry basis) % |
| 项目 Items | 20 cm组 Group 20 cm | 30 cm组 Group 30 cm | 40 cm组 Group 40 cm |
|---|---|---|---|
| 原料Ingredients | |||
| 留茬高度20 cm全株玉米青贮Whole-plant corn silage of stubble height 20 cm | 23.56 | ||
| 留茬高度30 cm全株玉米青贮Whole-plant corn silage of stubble height 30 cm | 24.17 | ||
| 留茬高度40 cm全株玉米青贮Whole-plant corn silage of stubble height 40 cm | 24.64 | ||
| 苜蓿干草Alfalfa hay | 10.59 | 10.51 | 10.44 |
| 全棉籽Whole cottonseed | 6.78 | 6.73 | 6.68 |
| 湿啤酒糟Wet brewer’s grains | 4.54 | 4.50 | 4.47 |
| 糖蜜豆皮Molasses bean peel | 3.76 | 3.73 | 3.71 |
| 玉米粉Corn meal | 28.47 | 28.24 | 28.07 |
| 豆粕Soybean meal | 10.73 | 10.65 | 10.58 |
| 菜籽粕Rapeseed meal | 2.14 | 2.12 | 2.11 |
| 玉米蛋白粉Corn gluten meal | 0.69 | 0.68 | 0.68 |
| 甘蔗糖蜜Cane molasses | 2.44 | 2.42 | 2.41 |
| 脂肪酸钙Fatty acid calcium | 1.63 | 1.62 | 1.61 |
| 石粉Limestone | 0.38 | 0.38 | 0.38 |
| 小苏打NaHCO3 | 0.31 | 0.31 | 0.30 |
| 食盐NaCl | 0.29 | 0.29 | 0.29 |
| 氧化镁MgO | 0.20 | 0.20 | 0.20 |
| 缓释尿素Slow-release urea | 0.50 | 0.49 | 0.49 |
| 葡萄糖Glucose | 1.15 | 1.14 | 1.13 |
| 预混料Premix1) | 1.84 | 1.82 | 1.81 |
| 合计Total | 100.00 | 100.00 | 100.00 |
| 营养水平Nutrient levels2) | |||
| 干物质DM | 48.22 | 48.52 | 48.71 |
| 粗蛋白质CP | 15.99 | 16.07 | 16.01 |
| 粗脂肪EE | 7.65 | 8.03 | 7.77 |
| 粗灰分Ash | 7.32 | 7.56 | 7.52 |
| 中性洗涤纤维NDF | 29.38 | 28.73 | 26.19 |
| 酸性洗涤纤维ADF | 17.57 | 17.49 | 16.13 |
| 淀粉Starch | 28.31 | 29.94 | 30.11 |
| 钙Ca | 0.61 | 0.60 | 0.66 |
| 磷P | 0.56 | 0.59 | 0.57 |
| 泌乳净能NEL/(MJ/kg) | 6.90 | 6.95 | 6.97 |
1)每千克预混料含有 One kg of the premix contained the following:VA 1 200 000 IU,VD 360 000 IU,VE 9 950 IU,Ca 139 g,Cu 3.4 g,Zn 10.4 g,Mn 8.0 g,Co 0.2 g,Fe 2.0 g。 2)泌乳净能为计算值,参照NRC(2001),其余营养水平为实测值。NEL was a calculated value, referring to NRC (2001), while the other nutrient levels were measured values. |
1.9.2 生产性能及乳成分的测定
1.10 数据处理及统计分析
2 结果
2.1 不同留茬高度对全株玉米原料营养成分的影响
表3 不同留茬高度对全株玉米原料营养成分的影响Table 3 Effects of different stubble heights on nutrient composition of whole-plant corn raw material % |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 干物质DM | 37.22±1.75 | 37.36±1.95 | 38.28±1.47 | 0.589 |
| 粗蛋白质CP | 5.60±0.23 | 6.38±0.85 | 6.28±0.61 | 0.136 |
| 中性洗涤纤维NDF | 36.60±0.48a | 33.36±1.87b | 31.41±2.31b | 0.002 |
| 酸性洗涤纤维ADF | 19.37±0.39a | 17.62±1.24b | 16.55±1.39b | 0.005 |
| 粗脂肪EE | 4.11±0.13a | 3.88±0.31a | 3.27±0.17b | <0.001 |
| 粗灰分Ash | 3.94±0.20 | 3.92±0.20 | 3.86±0.28 | 0.850 |
| 淀粉Starch | 36.79±1.35b | 40.97±1.62a | 41.41±1.81a | <0.001 |
| 水溶性碳水化合物WSC | 4.15±1.14b | 4.68±0.69b | 6.34±1.03a | <0.001 |
| 非纤维性碳水化合物NFC | 46.29±1.99b | 47.97±1.68a | 48.49±2.00a | 0.020 |
同行数据肩标不同小写字母表示差异显著(P<0.05),相同或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different small letter superscripts mean significant difference (P<0.05), while with the same or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.2 不同留茬高度对全株玉米青贮营养成分和RFV的影响
表4 不同留茬高度对全株玉米青贮营养成分和RFV的影响Table 4 Effects of different stubble heights on nutrient composition and RFV of whole-plant corn silage |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 干物质DM/% | 32.17±1.11 | 33.26±1.77 | 34.12±2.49 | 0.292 |
| 粗蛋白质CP/% | 7.27±0.49 | 7.68±0.17 | 7.22±0.30 | 0.114 |
| 中性洗涤纤维NDF/% | 35.62±2.91a | 35.00±1.17a | 32.00±1.17b | 0.012 |
| 酸性洗涤纤维ADF/% | 17.80±1.42a | 17.41±0.43a | 15.75±0.41b | 0.003 |
| 粗脂肪EE/% | 3.52±0.15 | 3.44±0.13 | 3.49±0.06 | 0.540 |
| 粗灰分Ash/% | 4.16±0.72a | 3.85±0.13b | 3.85±0.11b | <0.001 |
| 淀粉Starch/% | 35.86±1.82b | 38.14±2.52b | 41.44±2.34a | 0.007 |
| 水溶性碳水化合物WSC/% | 1.64±0.41b | 1.90±0.44b | 2.36±0.51a | <0.001 |
| 非纤维性碳水化合物NFC/% | 47.21±2.15b | 48.02±2.66b | 51.82±2.06a | <0.001 |
| 相对饲喂价值RFV | 197.28±8.92b | 200.44±7.55b | 223.03±8.72a | 0.006 |
2.3 不同留茬高度对全株玉米青贮发酵品质的影响
表5 不同留茬高度对全株玉米青贮发酵品质的影响Table 5 Effects of different stubble heights on fermentation quality of whole-plant corn silage |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| pH | 3.85±0.08 | 3.83±0.06 | 3.80±0.06 | 0.223 |
| 乳酸LA/% | 5.22±0.75 | 4.72±0.84 | 4.59±0.85 | 0.459 |
| 氨态氮NH3-N/(mmol/L) | 4.58±0.09 | 4.70±0.31 | 4.35±0.15 | 0.186 |
| 乙酸AA/(mmol/L) | 34.21±1.64b | 65.45±5.78a | 69.12±5.14a | <0.001 |
| 丙酸PA/(mmol/L) | 12.03±0.16b | 15.36±1.13a | 15.08±0.48a | <0.001 |
| 丁酸BA/(mmol/L) | 1.24±0.07a | 0.90±0.09b | 0.70±0.25b | 0.006 |
| 总挥发性脂肪酸TVFA/(mmol/L) | 53.06±1.81b | 88.28±7.70a | 90.71±6.23a | <0.001 |
2.4 不同留茬高度全株玉米青贮在奶牛瘤胃中的营养物质降解率及降解参数
2.4.1 不同留茬高度全株玉米青贮DM降解率及降解参数
表6 不同留茬高度全株玉米青贮DM降解率及降解参数Table 6 DM degradation rates and degradation parameters of whole-plant corn silage with different stubble heights |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 降解率Degradation rates/% | ||||
| 0 h | 17.98±1.56 | 19.24±2.66 | 21.23±3.20 | 0.230 |
| 4 h | 21.74±7.43 | 22.09±5.01 | 24.86±9.11 | 0.730 |
| 7 h | 23.79±1.35 | 23.44±2.18 | 26.48±3.32 | 0.326 |
| 12 h | 27.61±3.90 | 28.08±1.13 | 32.40±5.63 | 0.193 |
| 24 h | 38.27±7.28 | 38.90±5.70 | 43.95±9.42 | 0.410 |
| 30 h | 47.05±5.14 | 48.60±4.50 | 50.02±5.44 | 0.604 |
| 36 h | 48.68±1.66 | 50.00±5.94 | 55.42±6.11 | 0.083 |
| 48 h | 55.32±2.23b | 56.33±2.03b | 62.44±2.33a | <0.001 |
| 72 h | 60.14±2.04b | 62.72±2.59b | 70.46±4.34a | <0.001 |
| 降解参数Degradation parameters | ||||
| 快速降解部分a/% | 14.50±2.27 | 14.43±1.17 | 16.86±0.94 | 0.190 |
| 慢速降解部分b/% | 57.79±4.51 | 58.69±3.83 | 63.10±4.75 | 0.293 |
| 慢速降解部分的降解速率c/(%/h) | 0.027±0.011 | 0.025±0.005 | 0.024±0.006 | 0.864 |
| 有效降解率ED/% | 40.03±1.46b | 40.55±1.14b | 45.17±0.62a | 0.003 |
2.4.2 不同留茬高度全株玉米青贮NDF降解率及降解参数
表7 不同留茬高度全株玉米青贮NDF降解率及降解参数Table 7 NDF degradation rates and degradation parameters of whole-plant corn silage with different stubble heights |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 降解率Degradation rates/% | ||||
| 0 h | 8.68±0.46 | 9.54±1.93 | 8.39±3.17 | 0.744 |
| 4 h | 9.45±0.64 | 10.05±1.11 | 10.84±1.77 | 0.327 |
| 7 h | 12.71±2.22 | 13.58±1.46 | 14.32±2.13 | 0.464 |
| 12 h | 16.03±1.26 | 17.51±3.13 | 19.27±4.50 | 0.334 |
| 24 h | 31.55±1.91 | 32.61±6.35 | 35.07±8.82 | 0.640 |
| 30 h | 38.25±4.55 | 41.42±6.23 | 43.16±3.65 | 0.370 |
| 36 h | 41.84±2.64b | 42.12±0.65b | 46.53±0.47a | 0.004 |
| 48 h | 47.01±3.57b | 49.06±0.71b | 54.32±3.95a | 0.004 |
| 72 h | 53.80±4.68b | 55.82±1.79b | 61.54±4.11a | 0.014 |
| 降解参数Degradation parameters | ||||
| 快速降解部分a/% | 5.05±0.40 | 5.31±2.04 | 6.38±2.04 | 0.615 |
| 慢速降解部分b/% | 65.19±4.59 | 65.75±8.36 | 74.96±9.89 | 0.528 |
| 慢速降解部分的降解速率c/(%/h) | 0.023±0.008 | 0.024±0.009 | 0.021±0.004 | 0.834 |
| 有效降解率ED/% | 31.57±0.29b | 32.93±0.98b | 35.77±1.31a | 0.005 |
2.4.3 不同留茬高度全株玉米青贮ADF降解率及降解参数
表8 不同留茬高度全株玉米青贮ADF降解率及降解参数Table 8 ADF degradation rates and degradation parameters of whole-plant corn silage with different stubble heights |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 降解率Degradation rates/% | ||||
| 0 h | 9.26±2.70 | 9.12±0.41 | 9.42±2.33 | 0.986 |
| 4 h | 9.28±1.03 | 9.51±1.00 | 10.20±0.42 | 0.310 |
| 7 h | 13.11±2.37 | 13.66±2.02 | 15.03±1.39 | 0.306 |
| 12 h | 19.25±2.08 | 19.35±0.36 | 21.60±1.55 | 0.069 |
| 24 h | 25.47±1.95b | 26.16±2.25b | 31.51±2.19a | <0.001 |
| 30 h | 31.17±4.42 | 33.28±3.66 | 36.04±4.67 | 0.201 |
| 36 h | 39.70±0.85b | 40.28±1.26b | 44.61±3.21a | 0.003 |
| 48 h | 44.08±3.93b | 46.85±3.65ab | 49.83±3.80a | 0.018 |
| 72 h | 50.12±1.81b | 55.51±3.44a | 58.76±3.63a | 0.004 |
| 降解参数Degradation parameters | ||||
| 快速降解部分a/% | 6.37±1.35 | 6.54±0.23 | 7.30±0.78 | 0.486 |
| 慢速降解部分b/% | 64.12±10.75 | 77.57±10.83 | 74.51±5.14 | 0.180 |
| 慢速降解部分的降解速率c/(%/h) | 0.017±0.005 | 0.015±0.003 | 0.017±0.001 | 0.601 |
| 有效降解率ED/% | 28.52±2.13b | 30.94±1.00ab | 33.92±1.64a | 0.010 |
2.4.4 不同留茬高度全株玉米青贮Starch降解率及降解参数
表9 不同留茬高度全株玉米青贮Starch降解率及降解参数Table 9 Starch degradation rates and degradation parameters of whole-plant corn silage with different stubble heights |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 降解率Degradation rates/% | ||||
| 0 h | 40.63±8.28 | 39.96±8.85 | 38.45±9.42 | 0.919 |
| 4 h | 73.77±0.25 | 75.87±4.90 | 77.52±4.92 | 0.528 |
| 7 h | 78.52±2.64 | 79.09±3.07 | 81.10±3.75 | 0.471 |
| 12 h | 83.35±2.40 | 85.66±4.66 | 87.87±3.32 | 0.140 |
| 24 h | 88.81±3.97 | 90.04±2.52 | 92.51±1.59 | 0.300 |
| 30 h | 91.88±3.15 | 92.88±1.80 | 94.15±2.95 | 0.455 |
| 36 h | 94.87±1.80 | 95.44±5.48 | 96.94±1.48 | 0.672 |
| 48 h | 97.03±1.19 | 97.52±1.59 | 98.88±0.07 | 0.192 |
| 72 h | 98.93±0.74 | 99.04±0.44 | 99.73±0.25 | 0.249 |
| 降解参数Degradation parameters | ||||
| 快速降解部分a/% | 68.02±2.38 | 70.39±3.48 | 68.00±8.32 | 0.827 |
| 慢速降解部分b/% | 31.37±2.18 | 28.91±3.07 | 31.03±7.20 | 0.792 |
| 慢速降解部分的降解速率c/(%/h) | 0.046±0.008 | 0.047±0.014 | 0.077±0.049 | 0.406 |
| 有效降解率ED/% | 86.78±0.61b | 87.74±0.83b | 89.62±0.82a | 0.010 |
2.5 饲喂不同留茬高度全株玉米青贮对奶牛生产性能及乳成分的影响
表10 饲喂不同留茬高度全株玉米青贮对奶牛生产性能及乳成分的影响Table 10 Effects of feeding whole-plant corn silage with different stubble heights on performance and milk composition of dairy cows |
| 项目 Items | 留茬高度Stubble height/cm | P值 P-value | ||
|---|---|---|---|---|
| 20 | 30 | 40 | ||
| 产奶量Milk yield/(kg/d) | 42.80±1.94c | 44.13±2.71b | 47.14±2.58a | <0.001 |
| 4%乳脂校正乳4%FCM/kg | 37.60±1.70c | 40.50±2.48b | 45.22±2.48a | <0.001 |
| 能量校正乳ECM/kg | 41.35±1.87c | 44.48±2.73b | 49.48±2.71a | <0.001 |
| 饲料转化率FCR | 1.67±0.19b | 1.72±0.14b | 1.94±0.18a | <0.001 |
| 30 d泌乳持续力30 d lactation sustainability/% | 96.47±4.60 | 99.65±6.70 | 100.36±10.27 | 0.676 |
| 乳脂Milk Fat/% | 3.19±0.21c | 3.43±0.18b | 3.70±0.15a | <0.001 |
| 乳蛋白Milk protein/% | 3.05±0.25c | 3.21±0.22b | 3.27±0.10a | <0.001 |
| 乳糖Lactose/% | 5.34±0.27 | 5.43±0.14 | 5.35±0.15 | 0.254 |
| 乳尿素氮MUN/(mg/dL) | 10.84±2.74 | 9.66±1.50 | 10.79±3.02 | 0.183 |
| 体细胞数SCC/(×103个/mL) | 165.88±6.53 | 169.67±8.02 | 176.25±9.98 | 0.135 |

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