1 材料与方法
1.1 试验设计及样品采集
1.2 试验饲粮
表1 试验饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of experimental diets (air-dry basis) % |
| 项目 Items | 玉米饲粮Corn diet | 小麦饲粮Wheat diet | 高粱饲粮Sorghum diet | 花生粕饲粮Peanut meal diet | 混合饲粮Mixed diet | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | |||||
| 原料Ingredients | ||||||||||||||
| 玉米Corn | 94.90 | 95.00 | 22.90 | 22.30 | ||||||||||
| 小麦Wheat | 94.00 | 94.00 | 22.50 | 21.00 | ||||||||||
| 高粱Sorghum diet | 93.00 | 93.00 | 22.50 | 21.00 | ||||||||||
| 花生粕Peanut meal | 41.60 | 34.00 | 22.50 | 21.00 | ||||||||||
| 大豆油Soybean oil | 1.10 | 1.35 | 2.10 | 2.35 | 3.50 | 2.70 | 2.40 | 2.40 | ||||||
| 磷酸氢钙CaHPO4 | 1.80 | 1.70 | 1.50 | 1.35 | 2.00 | 1.85 | 2.00 | 2.00 | 1.70 | 1.60 | ||||
| 石粉Limestone | 1.50 | 1.50 | 1.60 | 1.50 | 1.10 | 1.00 | 1.10 | 1.00 | 1.30 | 1.20 | ||||
| 食盐NaCl | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | ||||
| 葡萄糖Glucose | 50.00 | 58.50 | 2.40 | 7.70 | ||||||||||
| 预混料Premix1) | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | ||||
| 二氧化钛TiO2 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | ||||
| 合计Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | ||||
| 营养水平Nutrient levels2) | ||||||||||||||
| 表观代谢能AME/(MJ/kg) | 12.35 | 12.39 | 12.37 | 12.46 | 12.22 | 12.31 | 12.27 | 12.24 | 12.26 | 12.32 | ||||
| 干物质DM | 91.70 | 90.70 | 91.89 | 91.69 | 91.61 | 91.42 | 96.68 | 96.68 | 93.09 | 93.19 | ||||
| 粗蛋白质CP | 7.80 | 7.68 | 13.25 | 13.47 | 8.65 | 8.80 | 13.96 | 12.27 | 14.63 | 13.85 | ||||
| 粗脂肪EE | 3.32 | 3.33 | 2.68 | 2.34 | 3.16 | 3.36 | 4.01 | 3.12 | 4.62 | 4.50 | ||||
| 粗灰分Ash | 4.94 | 5.13 | 5.14 | 5.30 | 4.82 | 4.77 | 6.13 | 6.51 | 5.69 | 5.86 | ||||
| 中性洗涤纤维NDF | 4.75 | 7.00 | 3.49 | 11.30 | 8.93 | 4.91 | 7.30 | 4.15 | 11.00 | 8.64 | ||||
| 酸性洗涤纤维ADF | 1.39 | 1.74 | 1.14 | 7.69 | 5.26 | 1.61 | 2.11 | 1.42 | 7.57 | 4.98 | ||||
| 钙Ca | 0.93 | 0.88 | 0.94 | 0.85 | 0.91 | 0.87 | 0.91 | 0.86 | 0.95 | 0.87 | ||||
| 总磷TP | 0.67 | 0.63 | 0.63 | 0.60 | 0.66 | 0.62 | 0.68 | 0.61 | 0.67 | 0.62 | ||||
| 总能GE/(MJ/kg) | 15.28 | 15.26 | 15.51 | 15.48 | 15.93 | 16.02 | 16.71 | 15.94 | 16.74 | 16.62 | ||||
1)预混料为每千克饲粮提供 The premix provided the following per kg of diets:17~20日龄 17 to 20 days of age,VA 10 000 IU,VD3 2 600 IU,VE 20 IU,VK3 2 mg,VB1 1.6 mg,VB2 6 mg,VB6 3 mg,VB12 0.012 mg,泛酸 pantothenic acid 20 mg,烟酸 nicotinic acid 30 mg,叶酸 folic acid 0.8 mg,生物素 biotin 0.12 mg,胆碱 choline 500 mg,Cu (as copper sulfate) 8 mg,Fe (as copper sulfate) 80 mg,Zn (as copper sulfate) 40 mg,Mn (as manganese sulfate) 80 mg,I (as potassium iodide) 0.35 mg,Se (as sodium selenite) 0.15 mg;38~41日龄 38 to 41 days of age,VA 4 000 IU,VD3 1 000 IU,VE 15 mg,VK3 1.5mg,VB1 3 mg,VB2 2 mg,泛酸 pantothenic acid 15 mg,烟酸 nicotinic acid 35 mg,VB6 3.5 mg,生物素 biotin 0.15 mg,叶酸 folic acid 0.8 mg,VB12 0.015 mg,Cu (as copper sulfate) 8 mg,Fe (as copper sulfate) 80 mg,Zn (as copper sulfate) 70 mg,Mn (as manganese sulfate) 80 mg,I (as potassium iodide) 0.7 mg,Se (as sodium selenite) 0.25 mg。 2)表观代谢能为计算值,参考《肉鸭饲养标准》(NY/T 2122—2012)计算,其余为实测值。表2同。AME was a calculated value, referred to Meat Duck Feeding Standard (NY/T 2122—2012), while the others were measured values. The same as Table 2. |
表2 商品饲粮组成及营养水平(风干基础)Table 2 Composition and nutrient levels of commercial diets (air-dry basis) % |
| 项目 Items | 1~16日龄 1 to 16 days of age | 22~37日龄 22 to 37 days of age |
|---|---|---|
| 原料Ingredients | ||
| 玉米Corn | 58.0 | 62.0 |
| 小麦麸Wheat bran | 8.0 | 11.3 |
| 大豆粕Soybean meal | 27.4 | 17.0 |
| 棉籽粕Cottonseed meal | 3.4 | |
| 大豆油Soybean oil | 2.0 | 2.0 |
| 磷酸氢钙CaHPO4 | 1.8 | 1.6 |
| 石粉Limestone | 1.5 | 1.4 |
| 食盐NaCl | 0.3 | 0.3 |
| 预混料Premix | 1.0 | 1.0 |
| 合计Total | 100.0 | 100.0 |
| 营养水平Nutrient levels | ||
| 表观代谢能AME/(MJ/kg) | 12.46 | 12.12 |
| 干物质DM | 91.33 | 91.87 |
| 粗蛋白质CP | 19.40 | 17.66 |
| 粗脂肪EE | 3.32 | 3.33 |
| 粗灰分Ash | 4.94 | 5.13 |
| 中性洗涤纤维NDF | 5.54 | 5.76 |
| 酸性洗涤纤维ADF | 1.58 | 1.64 |
| 钙Ca | 0.87 | 0.85 |
| 总磷TP | 0.67 | 0.63 |
| 总能GE/(MJ/kg) | 16.28 | 15.67 |
预混料为每千克饲粮提供 The premix provided the following per kg of diets:1~16日龄 1 to 16 days of age,VA 10 000 IU,VD3 2 600 IU,VE 20 IU,VK3 2 mg,VB1 1.6 mg,VB2 6 mg,VB6 3 mg,VB12 0.012 mg,泛酸 pantothenic acid 20 mg,烟酸 nicotinic acid 30 mg,叶酸 folic acid 0.8 mg,生物素 biotin 0.12 mg,胆碱 choline 500 mg,Cu (as copper sulfate) 8 mg,Fe (as copper sulfate) 80 mg,Zn (as copper sulfate) 40 mg,Mn (as manganese sulfate) 80 mg,I (as potassium iodide) 0.35 mg,Se (as sodium selenite) 0.15 mg;22~37日龄 22 to 37 days of age,VA 4 000 IU,VD3 1 000 IU,VE 15 mg,VK3 1.5mg,VB1 3 mg,VB2 2 mg,泛酸 pantothenic acid 15 mg,烟酸 nicotinic acid 35 mg,VB6 3.5 mg,生物素 biotin 0.15 mg,叶酸 folic acid 0.8 mg,VB12 0.015 mg,Cu (as copper sulfate) 8 mg,Fe (as copper sulfate) 80 mg,Zn (as copper sulfate) 70 mg,Mn (as manganese sulfate) 80 mg,I (as potassium iodide) 0.7 mg,Se (as sodium selenite) 0.25 mg。 |
表3 无氮饲粮组成(风干基础)Table 3 Composition of ingredients for nitrogen-free diets (air-dry basis) % |
| 项目Items | 含量Content |
|---|---|
| 葡萄糖Glucose | 63.30 |
| 玉米淀粉Corn starch | 21.10 |
| 大豆油Soybean oil | 3.50 |
| 纤维素Cellulose | 5.00 |
| 氯化胆碱Choline chloride | 0.50 |
| 碳酸钾K2CO3 | 0.30 |
| 碳酸氢钠NaHCO3 | 0.80 |
| 氯化钾KCl | 0.30 |
| 氧化镁MgO | 0.10 |
| 磷酸氢钙CaHPO4 | 2.70 |
| 石粉Limestone | 0.90 |
| 预混料Premix | 1.00 |
| 二氧化钛TiO2 | 0.50 |
| 合计Total | 100.00 |
预混料为每千克饲粮提供 The premix provided the following per kg of the diet:VA 10 000 IU,VD3 2 600 IU,VE 20 IU,VK3 2 mg,VB1 1.6 mg,VB2 6 mg,VB6 3 mg,VB12 0.012 mg,泛酸 pantothenic acid 20 mg,烟酸 nicotinic acid 30 mg,叶酸 folic acid 0.8 mg,生物素 biotin 0.12 mg,胆碱 choline 500 mg,Cu (as copper sulfate) 8 mg,Fe (as copper sulfate) 80 mg,Zn (as copper sulfate) 40 mg,Mn (as manganese sulfate) 80 mg,I (as potassium iodide) 0.35 mg,Se (as sodium selenite) 0.15 mg。 |
表4 试验饲粮中氨基酸含量(干物质基础)Table 4 Amino acid contents in experimental diets (DM basis) % |
| 项目 Items | 玉米饲粮Corn diet | 小麦饲粮Wheat diet | 高粱饲粮Sorghum diet | 花生粕饲粮Peanut meal diet | 混合饲粮Mixed diet | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | 17~20日龄 17 to 20 days of age | 38~41日龄 38 to 41 days of age | |||||
| 必需氨基酸EAA | ||||||||||||||
| 赖氨酸Lys | 0.23 | 0.21 | 0.33 | 0.30 | 0.19 | 0.17 | 0.39 | 0.34 | 0.41 | 0.36 | ||||
| 蛋氨酸Met | 0.15 | 0.14 | 0.18 | 0.17 | 0.14 | 0.13 | 0.14 | 0.12 | 0.19 | 0.17 | ||||
| 苏氨酸Thr | 0.25 | 0.24 | 0.34 | 0.32 | 0.27 | 0.25 | 0.34 | 0.30 | 0.41 | 0.36 | ||||
| 异亮氨酸Ile | 0.26 | 0.24 | 0.44 | 0.42 | 0.35 | 0.32 | 0.43 | 0.37 | 0.49 | 0.45 | ||||
| 亮氨酸Leu | 0.85 | 0.80 | 0.82 | 0.77 | 1.11 | 1.04 | 0.81 | 0.72 | 1.15 | 1.02 | ||||
| 苯丙氨酸Phe | 0.28 | 0.26 | 0.46 | 0.41 | 0.39 | 0.34 | 0.57 | 0.52 | 0.63 | 0.54 | ||||
| 缬氨酸Val | 0.35 | 0.35 | 0.54 | 0.53 | 0.44 | 0.42 | 0.53 | 0.44 | 0.63 | 0.57 | ||||
| 组氨酸His | 0.20 | 0.18 | 0.29 | 0.26 | 0.20 | 0.18 | 0.28 | 0.26 | 0.32 | 0.28 | ||||
| 精氨酸Arg | 0.28 | 0.27 | 0.51 | 0.49 | 0.29 | 0.26 | 1.20 | 1.09 | 0.95 | 0.86 | ||||
| 非必需氨基酸NEAA | ||||||||||||||
| 甘氨酸Gly | 0.29 | 0.27 | 0.52 | 0.49 | 0.29 | 0.27 | 0.71 | 0.64 | 0.66 | 0.60 | ||||
| 天冬氨酸Asp | 0.51 | 0.49 | 0.66 | 0.63 | 0.59 | 0.56 | 1.45 | 1.36 | 1.25 | 1.15 | ||||
| 丝氨酸Ser | 0.34 | 0.32 | 0.55 | 0.53 | 0.38 | 0.35 | 0.58 | 0.55 | 0.63 | 0.59 | ||||
| 谷氨酸Glu | 1.61 | 1.50 | 3.68 | 3.56 | 1.95 | 1.85 | 2.45 | 2.26 | 3.07 | 2.79 | ||||
| 丙氨酸Ala | 0.55 | 0.54 | 0.44 | 0.43 | 0.79 | 0.76 | 0.50 | 0.45 | 0.71 | 0.67 | ||||
| 半胱氨酸Cys | 0.16 | 0.16 | 0.28 | 0.27 | 0.16 | 0.15 | 0.19 | 0.16 | 0.25 | 0.23 | ||||
| 脯氨酸Pro | 0.59 | 0.82 | 1.17 | 1.55 | 0.69 | 0.90 | 0.61 | 0.52 | 0.95 | 1.18 | ||||
| 总氨基酸TAA | 6.89 | 6.76 | 11.28 | 11.13 | 8.21 | 7.94 | 11.18 | 10.12 | 12.71 | 11.82 | ||||
1.3 饲养管理
1.4 检测指标及计算方法
1.4.1 检测指标
1.4.2 计算方法
1.5 数据统计与分析
2 结果与分析
2.1 日龄对北京鸭回肠末端EAAL的影响
表5 北京鸭回肠末端EAAL(干物质基础)Table 5 EAAL in terminal ileum of Beijing ducks (DM basis) mg/kg |
| 项目 Items | 内源性氨基酸损失EAAL | SEM | P值 P-value | |
|---|---|---|---|---|
| 21日龄21 days of age | 42日龄42 days of age | |||
| 必需氨基酸EAA | ||||
| 赖氨酸Lys | 1 576.02 | 1 383.20 | 64.20 | 0.138 |
| 蛋氨酸Met | 490.45a | 375.74b | 23.23 | 0.008 |
| 苏氨酸Thr | 1 344.90 | 1 456.72 | 41.01 | 0.181 |
| 异亮氨酸Ile | 1 214.29 | 1 171.44 | 35.82 | 0.568 |
| 亮氨酸Leu | 2 040.50 | 1 929.58 | 64.48 | 0.408 |
| 苯丙氨酸Phe | 1 206.96 | 1 186.95 | 37.06 | 0.798 |
| 缬氨酸Val | 1 479.98 | 1 547.29 | 43.73 | 0.461 |
| 组氨酸His | 543.12 | 507.49 | 17.05 | 0.312 |
| 精氨酸Arg | 1 045.13a | 725.45b | 55.13 | 0.001 |
| 非必需氨基酸NEAA | ||||
| 甘氨酸Gly | 1 414.88 | 1 459.09 | 42.38 | 0.619 |
| 天冬氨酸Asp | 2 273.58 | 2 203.85 | 64.86 | 0.608 |
| 丝氨酸Ser | 1 116.06 | 1 228.23 | 40.34 | 0.172 |
| 谷氨酸Glu | 3 865.97 | 3 669.87 | 107.68 | 0.381 |
| 丙氨酸Ala | 1 563.06 | 1 452.68 | 47.08 | 0.255 |
| 半胱氨酸Cys | 465.55b | 737.15a | 39.20 | <0.001 |
| 脯氨酸Pro | 1 235.12b | 1 552.18a | 56.91 | 0.002 |
| 总氨基酸TAA | 22 875.59 | 24 082.32 | 395.05 | 0.131 |
2.2 日龄和饲粮类型对北京鸭氨基酸SID的影响
表6 21和42日龄北京鸭氨基酸SID(干物质基础)Table 6 Amino acid SID of Beijing ducks at 21 days and 42 days of age (DM basis) % |
| 项目 Items | 必需氨基酸EAA | 非必需氨基酸NEAA | 总氨基酸 TAA | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 赖氨酸 Lys | 蛋氨酸 Met | 苏氨酸 Thr | 异亮氨酸 Ile | 亮氨酸 Leu | 苯丙氨酸 Phe | 缬氨酸 Val | 组氨酸 His | 精氨酸 Arg | 甘氨酸 Gly | 天冬氨酸 Asp | 丝氨酸 Ser | 谷氨酸 Glu | 丙氨酸 Ala | 半胱氨酸 Cys | 脯氨酸 Pro | |||||
| 饲粮类型 Diet type | 日龄 Day of age | |||||||||||||||||||
| 玉米饲粮 Corn diet | 21 | 90.82 | 89.91 | 79.24 | 87.63 | 92.93 | 81.99 | 86.87 | 80.35 | 88.12 | 80.75 | 86.99 | 85.78 | 92.19 | 91.48 | 84.71 | 87.90 | 88.56 | ||
| 42 | 66.50 | 84.96 | 79.24 | 85.27 | 91.06 | 73.83 | 88.71 | 87.11 | 83.55 | 82.27 | 83.97 | 84.19 | 93.01 | 90.37 | 95.01 | 92.75 | 89.85 | |||
| 小麦饲粮 Wheat diet | 21 | 79.08 | 74.34 | 61.46 | 75.02 | 77.70 | 72.39 | 73.49 | 73.09 | 68.71 | 69.82 | 64.82 | 76.22 | 89.39 | 64.51 | 79.95 | 85.42 | 79.13 | ||
| 42 | 101.68 | 98.34 | 97.06 | 100.92 | 100.66 | 91.73 | 99.20 | 97.05 | 92.61 | 94.32 | 97.20 | 96.74 | 101.56 | 97.34 | 102.26 | 99.81 | 100.37 | |||
| 高粱饲粮 Sorghum diet | 21 | 102.13 | 101.79 | 95.14 | 100.23 | 98.60 | 93.78 | 98.49 | 89.75 | 99.35 | 94.75 | 99.71 | 94.56 | 98.52 | 98.98 | 88.38 | 90.78 | 97.19 | ||
| 42 | 126.85 | 104.07 | 104.36 | 106.66 | 100.48 | 91.81 | 105.94 | 97.24 | 103.97 | 103.5 | 106.74 | 100.45 | 100.59 | 100.75 | 104.76 | 96.36 | 103.36 | |||
| 花生粕饲粮 Peanut meal diet | 21 | 76.06 | 84.10 | 80.90 | 86.57 | 89.18 | 85.00 | 86.41 | 84.13 | 91.56 | 76.52 | 88.38 | 82.08 | 94.09 | 90.53 | 80.05 | 84.76 | 87.56 | ||
| 42 | 92.20 | 94.00 | 99.84 | 97.28 | 97.45 | 89.37 | 99.16 | 90.19 | 94.06 | 81.46 | 92.78 | 91.58 | 95.70 | 100.37 | 102.98 | 99.64 | 95.80 | |||
| 混合饲粮 Mixed diet | 21 | 97.87 | 92.45 | 80.88 | 88.80 | 91.49 | 84.64 | 87.64 | 82.50 | 92.14 | 76.96 | 88.54 | 84.36 | 92.72 | 91.22 | 80.75 | 86.00 | 88.81 | ||
| 42 | 97.39 | 97.33 | 94.50 | 92.14 | 97.49 | 92.96 | 98.17 | 83.35 | 92.32 | 87.27 | 93.49 | 92.3 | 96.10 | 96.85 | 97.13 | 94.30 | 95.62 | |||
| 主效应Main effects | ||||||||||||||||||||
| 日龄 Day of age | 21 | 89.19b | 88.52b | 79.52b | 87.65b | 89.98b | 83.56 | 86.58b | 81.96b | 87.98b | 79.76b | 85.69b | 84.60b | 93.38b | 87.34b | 82.77b | 86.97b | 88.25b | ||
| 42 | 96.92a | 95.74a | 95.00a | 96.45a | 97.43a | 87.94 | 98.24a | 90.99a | 93.30a | 89.76a | 94.84a | 93.05a | 97.39a | 97.14a | 100.43a | 96.57a | 97.00a | |||
| SEM | 1.48 | 1.58 | 1.96 | 1.47 | 1.07 | 1.39 | 1.41 | 1.158 | 1.39 | 1.56 | 1.58 | 1.12 | 0.68 | 1.47 | 1.31 | 0.88 | 1.05 | |||
| 饲粮 Diet | 玉米饲粮 Corn diet | 78.66c | 87.44b | 79.24b | 86.45b | 92.00b | 77.91c | 87.79b | 83.73b | 85.84bc | 81.51b | 85.48bc | 84.99b | 92.60b | 90.93b | 89.86b | 90.33 | 89.21b | ||
| 小麦饲粮 Wheat diet | 90.38bc | 86.34b | 79.26b | 87.97b | 89.18b | 82.06bc | 86.35b | 85.07b | 80.66c | 82.07b | 81.01c | 86.48b | 95.48b | 80.93c | 91.11b | 92.62 | 89.75b | |||
| 高粱饲粮 Sorghum diet | 114.49a | 102.93a | 99.75a | 103.45a | 99.54a | 92.80a | 102.22a | 93.50a | 101.66a | 99.13a | 103.23a | 97.51a | 99.56a | 99.87a | 96.57a | 93.57 | 100.28a | |||
| 花生粕饲粮 Peanut meal diet | 84.13bc | 89.05b | 90.37ab | 91.93b | 93.32ab | 87.19ab | 92.79b | 87.16ab | 92.81b | 78.99b | 90.58b | 86.83b | 94.90b | 95.45ab | 91.52b | 92.20 | 91.68b | |||
| 混合饲粮 Mixed diet | 97.63b | 94.89b | 87.69b | 90.47b | 94.49ab | 88.80ab | 92.91b | 82.93b | 92.23b | 82.12b | 91.015b | 88.33b | 94.41b | 94.04ab | 88.94b | 90.15 | 92.22b | |||
| SEM | 2.48 | 1.58 | 1.96 | 1.47 | 1.07 | 1.39 | 1.41 | 1.16 | 1.39 | 1.56 | 1.58 | 1.12 | 0.68 | 1.16 | 1.31 | 0.89 | 1.05 | |||
| P值 P-value | 日龄Day of age | 0.037 | 0.008 | <0.001 | <0.001 | <0.001 | 0.071 | <0.001 | <0.001 | 0.010 | <0.001 | <0.001 | <0.001 | 0.001 | <0.001 | <0.001 | <0.001 | <0.001 | ||
| 饲粮Diet | <0.001 | 0.001 | <0.001 | <0.001 | 0.004 | 0.002 | <0.001 | 0.003 | <0.001 | <0.001 | <0.001 | <0.001 | 0.005 | <0.001 | 0.039 | 0.424 | <0.001 | |||
| 日龄×饲粮 Days of age×diet | <0.001 | 0.015 | 0.005 | 0.003 | 0.004 | 0.007 | 0.011 | 0.002 | <0.001 | 0.037 | <0.001 | 0.003 | 0.005 | <0.001 | 0.099 | 0.048 | 0.002 | |||
同列数据肩标无字母或相同字母表示差异不显著(P>0.05),不同小写字母表示差异显著(P<0.05)。 | |
In the same row, values with no letter or the same letter superscripts mean no significant difference (P>0.05), while with different small letter superscripts mean significant difference (P<0.05). |
2.3 混合饲粮中北京鸭的氨基酸AID和SID的测定值与预测值
表7 21日龄混合饲粮中北京鸭的氨基酸AID和SID的测定值与预测值Table 7 Measured value and predicted value of amino acid AID and SID in mixed diets of Beijing ducks at 21 days of age % |
| 项目 Items | 标准回肠消化率SID | 表观回肠消化率AID | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 测定值 Measured value | 预测值 Predicted value | 差值 Difference | SEM | P值 P-value | 测定值 Measured value | 预测值 Predicted value | 差值 Difference | SEM | P值 P-value | |||
| 必需氨基酸EAA | ||||||||||||
| 赖氨酸Lys | 97.87 | 95.86 | 2.01 | 3.14 | 0.459 | 60.85a | 32.52b | 28.33 | 4.00 | <0.001 | ||
| 蛋氨酸Met | 92.45 | 86.44 | 6.01 | 4.84 | 0.254 | 67.30a | 53.48b | 13.82 | 2.54 | 0.001 | ||
| 苏氨酸Thr | 80.88 | 78.48 | 2.40 | 2.28 | 0.327 | 50.06a | 38.18b | 11.88 | 3.26 | 0.008 | ||
| 异亮氨酸Ile | 88.80 | 86.32 | 2.47 | 2.22 | 0.302 | 65.70a | 56.56b | 9.14 | 2.22 | 0.005 | ||
| 亮氨酸Leu | 91.49 | 89.94 | 1.56 | 2.14 | 0.491 | 74.68a | 68.00b | 6.68 | 1.54 | 0.003 | ||
| 苯丙氨酸Phe | 84.64 | 82.61 | 2.04 | 2.12 | 0.369 | 66.51a | 57.31b | 9.20 | 2.17 | 0.004 | ||
| 缬氨酸Val | 87.64 | 85.55 | 2.10 | 1.22 | 0.129 | 65.68a | 56.98b | 8.70 | 2.11 | 0.004 | ||
| 组氨酸His | 82.50 | 82.89 | -0.39 | 1.46 | 0.795 | 66.78a | 62.32b | 4.46 | 1.82 | 0.044 | ||
| 精氨酸Arg | 92.14 | 87.94 | 4.20 | 3.04 | 0.209 | 81.80a | 74.66b | 7.14 | 1.43 | 0.002 | ||
| 非必需氨基酸NEAA | ||||||||||||
| 甘氨酸Gly | 76.96 | 77.00 | -0.04 | 1.03 | 0.974 | 56.89a | 51.35b | 5.54 | 1.82 | 0.019 | ||
| 天冬氨酸Asp | 88.54 | 86.18 | 2.36 | 1.72 | 0.212 | 71.42a | 63.84b | 7.58 | 1.50 | 0.002 | ||
| 丝氨酸Ser | 84.36 | 82.92 | 1.44 | 3.48 | 0.691 | 67.95a | 61.77b | 6.18 | 1.97 | 0.016 | ||
| 谷氨酸Glu | 92.72 | 92.92 | -0.20 | 2.37 | 0.936 | 81.43a | 78.75b | 2.68 | 0.78 | 0.011 | ||
| 丙氨酸Ala | 91.22 | 88.66 | 2.56 | 1.52 | 0.137 | 70.59a | 62.29b | 8.30 | 1.90 | 0.003 | ||
| 半胱氨酸Cys | 80.75 | 81.74 | -0.99 | 1.56 | 0.547 | 63.54a | 59.83b | 3.71 | 1.36 | 0.029 | ||
| 脯氨酸Pro | 86.00 | 86.49 | -0.50 | 3.10 | 0.877 | 74.26a | 71.29b | 2.97 | 0.97 | 0.018 | ||
| 总氨基酸TAA | 88.81 | 87.31 | 1.50 | 1.42 | 0.325 | 72.04a | 65.40b | 6.64 | 1.59 | 0.004 | ||
表8 42日龄混合饲粮中北京鸭的氨基酸AID和SID的测定值与预测值Table 8 Measured value and predicted value of amino acid AID and SID in mixed diets of Beijing ducks at 42 days of age % |
| 项目 Items | 标准回肠消化率SID | 表观回肠消化率AID | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 测定值 Measured value | 预测值 Predicted value | 差值 Difference | SEM | P值 P-value | 测定值 Measured value | 预测值 Predicted value | 差值 Difference | SEM | P值 P-value | ||
| 必需氨基酸EAA | |||||||||||
| 赖氨酸Lys | 97.39 | 94.46 | 2.93 | 1.75 | 0.138 | 62.03a | 48.68b | 13.35 | 2.81 | 0.002 | |
| 蛋氨酸Met | 97.33 | 94.99 | 2.34 | 1.62 | 0.193 | 76.31a | 68.55b | 7.76 | 1.55 | 0.002 | |
| 苏氨酸Thr | 94.50 | 96.67 | -2.17 | 1.78 | 0.262 | 57.26a | 49.06b | 8.20 | 1.65 | 0.002 | |
| 异亮氨酸Ile | 92.14 | 93.17 | -1.03 | 3.53 | 0.780 | 68.12 | 66.54 | 1.58 | 2.40 | 0.531 | |
| 亮氨酸Leu | 97.49 | 97.51 | -0.01 | 1.53 | 0.993 | 80.10a | 75.30b | 4.76 | 1.48 | 0.015 | |
| 苯丙氨酸Phe | 92.96 | 87.93 | 5.03 | 3.52 | 0.196 | 72.60a | 61.03b | 11.57 | 3.41 | 0.012 | |
| 缬氨酸Val | 98.17 | 98.79 | -0.62 | 2.42 | 0.996 | 73.24a | 65.89b | 7.35 | 2.10 | 0.010 | |
| 组氨酸His | 83.35 | 88.82 | -5.47 | 2.96 | 0.107 | 66.80 | 71.68 | -4.88 | 2.48 | 0.090 | |
| 精氨酸Arg | 92.32 | 93.43 | -1.11 | 1.78 | 0.553 | 84.54 | 83.42 | 1.12 | 1.79 | 0.551 | |
| 非必需氨基酸NEAA | |||||||||||
| 甘氨酸Gly | 87.27 | 86.52 | 0.75 | 2.88 | 0.802 | 64.68a | 57.79b | 6.89 | 2.78 | 0.043 | |
| 天冬氨酸Asp | 93.49 | 94.11 | -0.62 | 1.12 | 0.597 | 75.77a | 71.29b | 4.48 | 1.06 | 0.004 | |
| 丝氨酸Ser | 92.30 | 93.05 | -0.75 | 1.28 | 0.578 | 73.02a | 68.45b | 4.57 | 1.22 | 0.007 | |
| 谷氨酸Glu | 96.10 | 97.92 | -1.82 | 1.42 | 0.240 | 83.97 | 82.98 | 0.99 | 0.62 | 0.152 | |
| 丙氨酸Ala | 96.85 | 98.03 | -1.18 | 1.54 | 0.468 | 76.69a | 72.27b | 4.42 | 0.79 | 0.001 | |
| 半胱氨酸Cys | 97.13b | 101.73a | -4.60 | 1.87 | 0.044 | 67.29 | 63.19 | 4.10 | 1.82 | 0.059 | |
| 脯氨酸Pro | 94.30 | 97.93 | -3.63 | 1.54 | 0.051 | 82.21a | 80.05b | 2.16 | 0.77 | 0.027 | |
| 总氨基酸TAA | 95.62 | 97.22 | -1.60 | 1.43 | 0.299 | 76.80a | 73.05b | 3.76 | 0.87 | 0.004 | |

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