1 材料与方法
1.1 伦理声明
1.2 试验设计
表1 1~21日龄金陵白鸭试验饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of experimental diets for Jinling white ducks aged from 1 to 21 days (air-dry basis) % |
| 项目 Items | 组别Groups | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | Ⅵ | Ⅶ | Ⅷ | Ⅸ | |
| 原料Ingredients | |||||||||
| 玉米Corn | 60.00 | 56.26 | 51.90 | 63.28 | 59.00 | 54.15 | 66.55 | 62.13 | 56.40 |
| 豆粕Soybean meal (43%) | 20.88 | 27.30 | 33.01 | 22.15 | 28.49 | 34.01 | 23.42 | 29.30 | 35.00 |
| 小麦麸Wheat bran | 14.50 | 12.09 | 11.01 | 9.45 | 7.39 | 7.00 | 4.39 | 3.28 | 3.00 |
| 磷酸氢钙CaHPO4 | 1.20 | 1.20 | 1.20 | 1.20 | 1.20 | 1.20 | 1.20 | 1.20 | 1.20 |
| 石粉Limestone | 1.36 | 1.36 | 1.36 | 1.28 | 1.28 | 1.27 | 1.19 | 1.19 | 1.19 |
| 食盐NaCl | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
| 豆油Soybean oil | 0.65 | 0.90 | 0.90 | 1.30 | 1.30 | 1.80 | |||
| DL-蛋氨酸DL-Met (99%) | 0.20 | 0.20 | 0.20 | 0.15 | 0.16 | 0.16 | 0.11 | 0.11 | 0.11 |
| L-赖氨酸L-Lys (70%) | 0.43 | 0.23 | 0.41 | 0.21 | 0.41 | 0.21 | |||
| L-苏氨酸L-Thr (99%) | 0.20 | 0.10 | 0.02 | 0.19 | 0.10 | 0.01 | 0.19 | 0.01 | |
| L-色氨酸L-Thy (99%) | 0.06 | 0.03 | 0.06 | 0.03 | 0.06 | 0.03 | |||
| 沸石粉Zeolite powder | 0.37 | 0.43 | 0.50 | 0.38 | 0.44 | 0.50 | 0.38 | 0.44 | 0.50 |
| 预混料Premix1) | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| 合计Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
| 营养水平Nutrient levels2) | |||||||||
| 代谢能ME/(MJ/kg) | 11.55 | 11.58 | 11.54 | 12.05 | 12.11 | 12.02 | 12.55 | 12.51 | 12.51 |
| 粗蛋白质CP | 15.82 | 17.97 | 20.65 | 15.83 | 17.96 | 19.44 | 15.89 | 17.72 | 20.36 |
| 粗纤维CF | 3.74 | 3.82 | 3.70 | 3.69 | 3.65 | 3.72 | 3.67 | 3.69 | 3.76 |
| 钙Ca | 1.05 | 1.04 | 1.07 | 1.06 | 1.09 | 1.10 | 1.09 | 1.03 | 1.04 |
| 有效磷AP | 0.43 | 0.43 | 0.44 | 0.42 | 0.43 | 0.43 | 0.42 | 0.42 | 0.43 |
| 赖氨酸Lys | 1.16 | 1.14 | 1.12 | 1.10 | 1.18 | 1.10 | 1.11 | 1.08 | 1.15 |
| 蛋氨酸Met | 0.46 | 0.45 | 0.50 | 0.44 | 0.47 | 0.46 | 0.41 | 0.40 | 0.46 |
1)预混料为每千克饲粮提供The premix provided the following per kg of diets:VA 8 000 IU,VD3 2 000 IU,VE 20 IU,VK3 2 mg,VB1 2 mg,VB2 10 mg,VB6 4 mg,VB12 0.02 mg,烟酸 nicotinic acid 50 mg,D-泛酸 D-pantothenic acid 20 mg,叶酸 folic acid 1 mg,胆碱 choline 1 g,生物素 biotin 0.15 mg,Fe (as ferrous sulfate) 60 mg,Cu (as copper sulfate) 8 mg,Mn (as manganese sulfate) 100 mg,Zn (as zinc sulfate) 60 mg,I (as potassium iodide) 0.4 mg,Se (as sodium selenite) 0.3 mg。 |
表2 22~56日龄金陵白鸭试验饲粮组成及营养水平(风干基础)Table 2 Composition and nutrient levels of experimental diets for Jinling white ducks aged from 22 to 56 days (air-dry basis) % |
| 项目 Items | 组别Groups | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | Ⅵ | Ⅶ | Ⅷ | Ⅸ | |
| 原料Ingredients | |||||||||
| 玉米Corn | 64.50 | 60.30 | 56.00 | 67.50 | 63.75 | 58.95 | 68.60 | 65.00 | 63.00 |
| 豆粕Soybean meal (43%) | 16.50 | 22.80 | 29.10 | 16.23 | 22.22 | 28.66 | 17.00 | 23.00 | 29.33 |
| 小麦麸Wheat bran | 12.00 | 9.00 | 7.00 | 10.60 | 9.00 | 6.80 | 8.05 | 6.09 | 3.00 |
| 磷酸氢钙CaHPO4 | 1.10 | 1.12 | 1.15 | 1.13 | 1.15 | 1.17 | 1.16 | 1.18 | 1.20 |
| 石粉Limestone | 1.46 | 1.29 | 1.11 | 1.41 | 1.25 | 1.08 | 1.37 | 1.21 | 1.05 |
| 食盐NaCl | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
| 豆油Soybean oil | 0.20 | 0.40 | 0.65 | 0.50 | 0.70 | 1.80 | 1.80 | 1.00 | |
| DL-蛋氨酸DL-Met (99%) | 0.20 | 0.16 | 0.12 | 0.20 | 0.16 | 0.12 | 0.20 | 0.16 | 0.12 |
| L-赖氨酸L-Lys(70%) | 0.40 | 0.20 | 0.41 | 0.22 | 0.41 | 0.21 | |||
| L-苏氨酸L-Thr (99%) | 0.17 | 0.09 | 0.18 | 0.04 | 0.18 | 0.09 | |||
| L-色氨酸L-Thy (99%) | 0.05 | 0.02 | 0.04 | 0.02 | 0.04 | 0.02 | |||
| 沸石粉Zeolite powder | 2.82 | 4.02 | 4.32 | 0.85 | 0.89 | 1.72 | 0.39 | 0.44 | 0.50 |
| 预混料Premix1) | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| 合计Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
| 营养水平Nutrient levels2) | |||||||||
| 代谢能ME/(MJ/kg) | 11.53 | 11.51 | 11.55 | 12.06 | 12.04 | 12.01 | 12.55 | 12.51 | 12.50 |
| 粗蛋白质CP | 14.26 | 15.98 | 17.83 | 13.96 | 16.36 | 17.86 | 13.93 | 16.06 | 18.06 |
| 粗纤维CF | 3.22 | 3.21 | 3.37 | 3.29 | 3.25 | 3.31 | 3.26 | 3.23 | 3.30 |
| 钙Ca | 0.89 | 0.94 | 0.84 | 0.94 | 0.83 | 0.91 | 0.86 | 0.90 | 0.95 |
| 有效磷AP | 0.39 | 0.40 | 0.41 | 0.40 | 0.41 | 0.42 | 0.40 | 0.41 | 0.42 |
| 赖氨酸Lys | 0.98 | 0.96 | 0.93 | 0.98 | 0.96 | 0.98 | 0.96 | 0.98 | 0.95 |
| 蛋氨酸Met | 0.40 | 0.39 | 0.36 | 0.38 | 0.40 | 0.38 | 0.40 | 0.37 | 0.41 |
1)预混料为每千克饲粮提供 The premix provided the following per kg of diets:VA 8 000 IU,VD3 2 000 IU,VE 20 IU,VK3 2 mg,VB1 1.5 mg,VB2 10 mg,VB6 3 mg,VB12 0.02 mg,烟酸 nicotinic acid 50 mg,D-泛酸 D-pantothenic acid 10 mg,叶酸 folic acid 1 mg,胆碱 choline 1 g,生物素 biotin 0.15 mg,Fe (as ferrous sulfate) 60 mg,Cu (as copper sulfate) 8 mg,Mn (as manganese sulfate) 100 mg,Zn (as zinc sulfate) 60 mg,I (as potassium iodide) 0.4 mg,Se (as sodium selenite) 0.3 mg。 |
1.3 饲养管理
1.4 样品采集及指标测定
1.4.1 饲粮营养水平
1.4.2 生长性能
1.4.3 体尺性状
1.4.4 屠宰性能
1.5 数据统计与分析
2 结果与分析
2.1 饲粮代谢能和粗蛋白质水平对1~21日龄金陵白鸭生长性能的影响
表3 饲粮代谢能和粗蛋白质水平对1~21日龄金陵白鸭生长性能的影响Table 3 Effects of dietary ME and CP levels on growth performance of Jinling white ducks aged from 1 to 21 days |
| 组别 Groups | 代谢能 ME/(MJ/kg) | 粗蛋白质 CP/% | 初始体重 IBW/g | 21日龄体重 BW at 21 days of age/g | 平均日 采食量 ADFI/(g/d) | 平均日增重 ADG/(g/d) | 料重比 F/G | |
|---|---|---|---|---|---|---|---|---|
| Ⅰ | 11.5 | 16.0 | 45.58 | 857.20 | 98.98a | 38.63 | 2.56a | |
| Ⅱ | 11.5 | 18.0 | 45.76 | 819.50 | 85.21ab | 36.85 | 2.31b | |
| Ⅲ | 11.5 | 20.0 | 45.58 | 840.00 | 88.40b | 37.82 | 2.33b | |
| Ⅳ | 12.0 | 16.0 | 45.76 | 836.17 | 81.29abc | 37.63 | 2.16bc | |
| Ⅴ | 12.0 | 18.0 | 45.71 | 839.00 | 78.46abc | 37.77 | 2.08c | |
| Ⅵ | 12.0 | 20.0 | 45.83 | 839.67 | 76.73bc | 37.80 | 2.03c | |
| Ⅶ | 12.5 | 16.0 | 45.67 | 858.33 | 77.31bc | 38.70 | 2.00c | |
| Ⅷ | 12.5 | 18.0 | 45.75 | 864.50 | 77.99bc | 38.99 | 2.00c | |
| Ⅸ | 12.5 | 20.0 | 45.67 | 827.00 | 73.05c | 37.21 | 1.96c | |
| SEM | 0.025 | 5.088 | 1.175 | 0.242 | 0.030 | |||
| 主效应Main effect | ||||||||
| 代谢能 ME/(MJ/kg) | 11.5 | 45.64 | 838.90 | 90.87Aa | 37.76 | 2.40Aa | ||
| 12.0 | 45.77 | 838.28 | 78.83Bb | 37.73 | 2.09Bb | |||
| 12.5 | 45.69 | 849.94 | 76.12Bb | 38.30 | 1.99Bb | |||
| 粗蛋白质 CP/% | 16.0 | 45.67 | 850.57 | 85.86Aa | 38.32 | 2.24Aa | ||
| 18.0 | 45.74 | 841.00 | 80.55ABab | 37.87 | 2.13Bb | |||
| 20.0 | 45.69 | 835.56 | 79.39Bb | 37.61 | 2.11Bb | |||
| P值P-value | ||||||||
| 代谢能ME | 0.118 | 0.580 | <0.001 | 0.565 | <0.001 | |||
| 粗蛋白质CP | 0.500 | 0.484 | 0.002 | 0.485 | 0.003 | |||
| 代谢能×粗蛋白质ME×CP | 0.319 | 0.300 | 0.030 | 0.301 | 0.097 | |||
同列数据肩标无字母或相同小写字母表示差异不显著(P>0.05),不同小写字母表示差异显著(P<0.05),不同大写字母表示差异极显著(P<0.01)。下表同。 | |
In the same column, values with no letter or the same small letter superscripts mean no significant difference (P>0.05), while with different small letter superscripts mean significant difference (P<0.05), and with different capital letter superscripts mean significant difference (P<0.01). The same as below. |
2.2 饲粮代谢能和粗蛋白质水平对22~56日龄金陵白鸭生长性能的影响
表4 饲粮代谢能和粗蛋白质水平对22~56日龄金陵白鸭生长性能的影响Table 4 Effects of dietary ME and CP levels on growth performance of Jinling white ducks aged from 22 to 56 days |
| 组别 Groups | 代谢能 ME/(MJ/kg) | 粗蛋白质 CP/% | 初始体重 IBW/g | 56日龄体重 BW at 56 days of age/g | 平均日 采食量 ADFI/(g/d) | 平均日增重 ADG/(g/d) | 料重比 F/G |
|---|---|---|---|---|---|---|---|
| Ⅰ | 11.5 | 14.0 | 832.50 | 2 439.24ab | 214.47a | 45.91ab | 4.68a |
| Ⅱ | 11.5 | 16.0 | 832.50 | 2 447.50ab | 215.66a | 46.14ab | 4.67a |
| Ⅲ | 11.5 | 18.0 | 834.17 | 2 447.00ab | 217.58a | 46.08ab | 4.73a |
| Ⅳ | 12.0 | 14.0 | 834.17 | 2 547.11a | 209.54ab | 48.95a | 4.31ab |
| Ⅴ | 12.0 | 16.0 | 834.17 | 2 438.80ab | 205.00ab | 45.85ab | 4.47ab |
| Ⅵ | 12.0 | 18.0 | 834.17 | 2 555.00a | 209.87ab | 49.17a | 4.27ab |
| Ⅶ | 12.5 | 14.0 | 831.67 | 2 470.83ab | 203.76ab | 46.83ab | 4.35ab |
| Ⅷ | 12.5 | 16.0 | 834.17 | 2 481.57ab | 195.01bc | 47.07ab | 4.16b |
| Ⅸ | 12.5 | 18.0 | 833.33 | 2 381.48b | 183.30c | 44.23b | 4.15b |
| SEM | 0.319 | 12.925 | 1.894 | 0.369 | 0.044 | ||
| 主效应Main effect | |||||||
| 代谢能 ME/(MJ/kg) | 11.5 | 833.06 | 2 444.58b | 215.90Aa | 46.04b | 4.70Aa | |
| 12.0 | 834.17 | 2 513.64a | 208.13Aa | 47.99a | 4.35Bb | ||
| 12.5 | 833.06 | 2 444.63b | 194.02Bb | 46.04b | 4.22Bb | ||
| 粗蛋白质 CP/% | 14.0 | 832.78 | 2485.73 | 209.26 | 47.23 | 4.45 | |
| 16.0 | 833.61 | 2455.96 | 205.22 | 46.35 | 4.43 | ||
| 18.0 | 833.89 | 2461.16 | 203.58 | 46.49 | 4.38 | ||
| P值P-value | |||||||
| 代谢能ME | 0.268 | 0.029 | <0.001 | 0.032 | <0.001 | ||
| 粗蛋白质CP | 0.341 | 0.546 | 0.223 | 0.524 | 0.761 | ||
| 代谢能×粗蛋白质ME×CP | 0.503 | 0.047 | 0.041 | 0.048 | 0.444 | ||
2.3 饲粮代谢能和粗蛋白质水平对56日龄金陵白鸭体尺性状的影响
表5 饲粮代谢能和粗蛋白质水平对56日龄金陵白鸭体尺性状的影响Table 5 Effects of dietary ME and CP levels on body size traits of Jinling white ducks aged 56 days cm |
| 组别 Groups | 代谢能 ME/(MJ/kg) | 粗蛋白质 CP/% | 体斜长 Body slope length | 胸深 Breast depth | 胸宽 Breast width | 半潜水长 Half-diving depth | 龙骨长 Fossil bone length | 胫长 Tibial length | 胫围 Tibial circumference |
|---|---|---|---|---|---|---|---|---|---|
| Ⅰ | 11.5 | 14.0 | 23.30 | 8.61ab | 9.84ab | 52.17 | 13.45 | 8.22 | 4.71 |
| Ⅱ | 11.5 | 16.0 | 23.45 | 8.59ab | 9.67ab | 52.42 | 13.40 | 8.17 | 4.66 |
| Ⅲ | 11.5 | 18.0 | 23.68 | 8.59ab | 8.75b | 52.45 | 12.28 | 8.44 | 4.71 |
| Ⅳ | 12.0 | 14.0 | 23.67 | 8.11b | 9.04ab | 52.68 | 13.28 | 8.19 | 4.77 |
| Ⅴ | 12.0 | 16.0 | 22.60 | 8.99a | 9.44ab | 52.25 | 13.60 | 8.20 | 4.78 |
| Ⅵ | 12.0 | 18.0 | 23.83 | 8.78ab | 10.02ab | 52.17 | 13.43 | 8.05 | 4.78 |
| Ⅶ | 12.5 | 14.0 | 23.40 | 8.42ab | 9.84ab | 51.95 | 13.10 | 8.19 | 4.69 |
| Ⅷ | 12.5 | 16.0 | 23.22 | 8.98a | 10.11a | 51.95 | 13.30 | 8.28 | 4.70 |
| Ⅸ | 12.5 | 18.0 | 23.77 | 8.83ab | 9.94ab | 51.20 | 13.18 | 8.07 | 4.73 |
| SEM | 0.147 | 0.067 | 0.104 | 0.174 | 0.076 | 0.036 | 0.023 | ||
| 主效应Main effect | |||||||||
| 代谢能 ME/(MJ/kg) | 11.5 | 23.48 | 8.59 | 9.42b | 52.34 | 13.38 | 8.27 | 4.69 | |
| 12.0 | 23.37 | 8.63 | 9.50ab | 53.37 | 13.44 | 8.14 | 4.78 | ||
| 12.5 | 23.46 | 8.74 | 9.95a | 51.70 | 13.18 | 8.18 | 4.71 | ||
| 粗蛋白质 CP/% | 14.0 | 23.46 | 8.38Bb | 9.57 | 52.27 | 13.28 | 8.20 | 4.72 | |
| 16.0 | 23.08 | 8.85Aa | 9.72 | 52.21 | 13.45 | 8.22 | 4.71 | ||
| 18.0 | 23.76 | 8.73Aa | 9.57 | 51.94 | 13.30 | 8.19 | 4.74 | ||
| P值P-value | |||||||||
| 代谢能ME | 0.948 | 0.536 | 0.042 | 0.237 | 0.439 | 0.326 | 0.328 | ||
| 粗蛋白质CP | 0.199 | 0.004 | 0.687 | 0.732 | 0.695 | 0.949 | 0.889 | ||
| 代谢能×粗蛋白质ME×CP | 0.700 | 0.109 | 0.010 | 0.849 | 0.946 | 0.170 | 0.989 | ||
2.4 饲粮代谢能和粗蛋白质水平对56日龄金陵白鸭屠宰性能的影响
表6 饲粮代谢能和粗蛋白质水平对56日龄金陵白鸭屠宰性能的影响Table 6 Effects of dietary ME and CP levels on slaughter performance of Jinling white ducks aged 56 days |
| 组别 Groups | 代谢能 ME/(MJ/kg) | 粗蛋白质 CP | 屠宰率 Dressing percentage/% | 半净膛率 Semi- eviscerated percentage/% | 全净膛率 Eviscerated percentage/% | 胸肌率 Breast muscle percentage/% | 腿肌率 Thigh muscle percentage/% | 腹脂率 Abdominal fat percentage/ % | 皮脂率 Subcutaneous fat percentage/ % | 胸肌厚度 Breast muscle thickness/cm |
|---|---|---|---|---|---|---|---|---|---|---|
| Ⅰ | 11.5 | 14.0 | 89.06ab | 81.81 | 75.80 | 14.10 | 11.27a | 1.02b | 21.13b | 1.61 |
| Ⅱ | 11.5 | 16.0 | 87.47ab | 81.09 | 74.60 | 14.27 | 9.48ab | 1.71ab | 24.83ab | 1.64 |
| Ⅲ | 11.5 | 18.0 | 86.86b | 80.56 | 74.56 | 13.40 | 10.27ab | 1.26ab | 23.08ab | 1.57 |
| Ⅳ | 12.0 | 14.0 | 89.86a | 80.44 | 74.29 | 13.10 | 9.85ab | 1.41ab | 24.28ab | 1.59 |
| Ⅴ | 12.0 | 16.0 | 87.72ab | 81.89 | 75.70 | 14.26 | 10.64ab | 1.17ab | 21.68ab | 1.58 |
| Ⅵ | 12.0 | 18.0 | 87.99ab | 81.62 | 75.65 | 14.60 | 9.28b | 1.22ab | 24.52ab | 1.58 |
| Ⅶ | 12.5 | 14.0 | 87.19ab | 80.90 | 74.43 | 13.92 | 9.29b | 1.92a | 26.41a | 1.49 |
| Ⅷ | 12.5 | 16.0 | 89.04ab | 82.40 | 77.20 | 14.37 | 9.77ab | 1.41ab | 22.57ab | 1.63 |
| Ⅸ | 12.5 | 18.0 | 88.39ab | 82.14 | 75.87 | 13.72 | 10.14ab | 1.58ab | 23.97ab | 1.56 |
| SEM | 0.231 | 0.217 | 0.239 | 0.160 | 0.157 | 0.065 | 0.410 | 0.027 | ||
| 主效应Main effect | ||||||||||
| 代谢能 ME/(MJ/kg) | 11.5 | 87.80 | 81.15 | 74.92 | 13.93 | 10.34 | 1.33ab | 23.01 | 1.61 | |
| 12.0 | 88.52 | 81.32 | 75.21 | 13.99 | 9.92 | 1.27b | 23.49 | 1.58 | ||
| 12.5 | 88.21 | 81.81 | 75.83 | 14.00 | 9.73 | 1.64a | 23.32 | 1.56 | ||
| 粗蛋白质 CP/% | 14.0 | 88.70 | 81.05 | 74.77 | 13.71 | 10.14 | 1.45 | 23.94 | 1.56 | |
| 16.0 | 88.08 | 81.79 | 75.83 | 14.30 | 9.96 | 1.43 | 23.03 | 1.61 | ||
| 18.0 | 87.75 | 81.44 | 75.36 | 13.91 | 9.90 | 1.35 | 23.86 | 1.57 | ||
| P值P-value | ||||||||||
| 代谢能ME | 0.372 | 0.428 | 0.242 | 0.978 | 0.217 | 0.028 | 0.372 | 0.824 | ||
| 粗蛋白质CP | 0.174 | 0.373 | 0.161 | 0.309 | 0.781 | 0.771 | 0.559 | 0.726 | ||
| 代谢能×粗蛋白质ME×CP | 0.012 | 0.222 | 0.071 | 0.225 | 0.009 | 0.020 | 0.015 | 0.896 | ||
2.5 饲粮代谢能和粗蛋白质水平对56日龄金陵白鸭内脏器官指数的影响
表7 饲粮代谢能和粗蛋白质水平对56日龄金陵白鸭内脏器官指数的影响Table 7 Effects of dietary energy and crude protein levels on visceral organ indexes of Jinling white ducks aged 56 days g/kg |
| 组别 Groups | 代谢能 ME/(MJ/kg) | 粗蛋白质 CP/% | 心脏指数 Heart index | 肝脏指数 Liver index | 肌胃指数 Gizzard index | 腺胃指数 Proventriculus index |
|---|---|---|---|---|---|---|
| Ⅰ | 11.5 | 14.0 | 5.80ABab | 21.29 | 23.48 | 3.25 |
| Ⅱ | 11.5 | 16.0 | 5.96Aa | 19.53 | 23.36 | 3.37 |
| Ⅲ | 11.5 | 18.0 | 5.35ABab | 19.72 | 22.25 | 3.33 |
| Ⅳ | 12.0 | 14.0 | 5.02Bb | 19.85 | 23.10 | 3.57 |
| Ⅴ | 12.0 | 16.0 | 5.50ABab | 21.25 | 23.15 | 3.13 |
| Ⅵ | 12.0 | 18.0 | 5.40ABab | 18.52 | 23.07 | 3.42 |
| Ⅶ | 12.5 | 14.0 | 5.51ABab | 18.72 | 22.67 | 3.61 |
| Ⅷ | 12.5 | 16.0 | 5.03Bb | 20.16 | 22.69 | 3.12 |
| Ⅸ | 12.5 | 18.0 | 5.64ABab | 18.18 | 23.56 | 3.33 |
| SEM | 0.067 | 0.349 | 0.257 | 0.073 | ||
| 主效应Main effect | ||||||
| 代谢能 ME/(MJ/kg) | 11.5 | 5.70a | 20.18 | 23.03 | 3.32 | |
| 12.0 | 5.31b | 19.87 | 23.11 | 3.37 | ||
| 12.5 | 5.39ab | 19.02 | 22.97 | 3.35 | ||
| 粗蛋白质 CP/% | 14.0 | 5.44 | 19.95 | 23.08 | 3.48 | |
| 16.0 | 5.49 | 20.31 | 23.07 | 3.21 | ||
| 18.0 | 5.46 | 18.81 | 22.96 | 3.36 | ||
| P值P-value | ||||||
| 代谢能ME | 0.021 | 0.371 | 0.980 | 0.963 | ||
| 粗蛋白质CP | 0.936 | 0.189 | 0.980 | 0.348 | ||
| 代谢能×粗蛋白质ME×CP | 0.004 | 0.454 | 0.717 | 0.678 | ||

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