1 材料与方法
1.1 伦理声明
1.2 试验材料
表1 粗糙米、糙米与玉米常规营养成分及毒素含量比较(风干基础)Table 1 Comparison on nutritional components and toxin contents of partially husked rice, brown rice and corn (air-dry basis) % |
| 项目 Items | 玉米 Corn | 粗糙米 Partially husked rice | 糙米 Brown rice |
|---|---|---|---|
| 水分Moisture | 13.9 | 14.9 | 15.0 |
| 粗蛋白质CP | 7.2 | 7.6 | 7.6 |
| 粗脂肪EE | 3.5 | 1.8 | 1.5 |
| 粗纤维CF | 2.1 | 1.6 | 0.7 |
| 粗灰分Ash | 1.3 | 1.2 | 0.8 |
| 总磷TP | 0.26 | 0.22 | 0.18 |
| 酸性洗涤纤维ADF | 1.8 | 1.7 | 0.8 |
| 中性洗涤纤维NDF | 7.0 | 2.9 | 1.2 |
| 淀粉Starch | 65.0 | 71.2 | 74.7 |
| 玉米赤霉烯酮Zearalenone/(μg/kg) | <20.0 | <20.0 | <20.0 |
| 呕吐毒素Vomitoxin/(mg/kg) | 0.4 | <0.2 | <0.2 |
| 黄曲霉毒素B1 Aflatoxin B1/(μg/kg) | <2.0 | <2.0 | <2.0 |
1.3 试验设计与饲粮
表2 试验饲粮组成及营养水平(风干基础)Table 2 Composition and nutrient levels of experimental diets (air-dry basis) % |
| 项目 Items | 12~24日龄12 to 24 days of age | 25~38日龄25 to 38 days of age | |||||
|---|---|---|---|---|---|---|---|
| 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | ||
| 原料Ingredients | |||||||
| 玉米Corn | 47.77 | 27.65 | 27.94 | 41.54 | 11.36 | 11.80 | |
| 粗糙米Partially husked rice | 20.00 | 30.00 | |||||
| 糙米Brown rice | 20.00 | 30.00 | |||||
| 豆粕Soybean meal | 26.89 | 26.76 | 26.71 | 22.61 | 22.40 | 22.32 | |
| 面粉Flour | 10.00 | 10.00 | 10.00 | 15.00 | 15.00 | 15.00 | |
| 花生粕Peanut meal | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 | |
| 玉米蛋白粉Corn gluten meal | 3.00 | 3.00 | 3.00 | 4.50 | 4.50 | 4.50 | |
| 豆油Soybean oil | 2.56 | 2.81 | 2.56 | 5.95 | 6.32 | 5.95 | |
| 玉米干酒糟及其可溶物Corn DDGS | 2.00 | 2.00 | 2.00 | 3.00 | 3.00 | 3.00 | |
| 石粉Limestone | 1.30 | 1.28 | 1.28 | 1.16 | 1.13 | 1.12 | |
| 磷酸氢钙CaHPO4 | 0.87 | 0.92 | 0.93 | 0.65 | 0.73 | 0.75 | |
| L-赖氨酸硫酸盐L-Lys·H2SO4 | 0.58 | 0.55 | 0.55 | 0.62 | 0.58 | 0.58 | |
| 食盐NaCl | 0.28 | 0.28 | 0.28 | 0.26 | 0.26 | 0.26 | |
| DL-蛋氨酸DL-Met | 0.25 | 0.25 | 0.25 | 0.21 | 0.22 | 0.22 | |
| 预混料Premix1) | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | |
| 合计Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | |
| 营养水平Nutrient levels2) | |||||||
| 粗蛋白质CP | 22.74 | 22.17 | 22.49 | 21.92 | 21.76 | 22.26 | |
| 代谢能ME/(MJ/kg) | 12.14 | 12.14 | 12.14 | 13.10 | 13.10 | 13.10 | |
| 粗纤维CF | 2.52 | 2.61 | 2.40 | 2.43 | 2.70 | 2.11 | |
1)预混料为每千克饲粮提供The premix provided the following per kg of diets:Mg 100 mg,Zn 75 mg,Fe 75 mg,I 0.65 mg,Cu 8 mg,Se 0.35 mg,VA 9 000 IU,VD3 2 000 IU,VE 20 IU,VK3 3.1 mg,VB1 1.2 mg,VB2 6.0 mg,烟酸 nicotinic acid 66 mg,泛酸 pantothenic acid 10 mg,VB6 2.6 mg,生物素 biotin 0.1 mg,叶酸 folic acid 0.7 mg,VB12 0.02 mg。 |
1.4 饲养管理
1.5 测定指标及方法
1.5.1 生长性能
1.5.2 屠宰性能
屠宰率(%)=(屠体重/宰前活重)×100;
全净膛率(%)=(全净膛重/宰前活重)×100;
胸肌率(%)=(双侧胸肌重/全净膛重)×100;
腿比率(%)=(双侧腿重/全净膛重)×100;
翅膀率(%)=(双侧翅膀/全净膛重)×100;
腹脂率(%)=[腹脂重/(全净膛重+腹脂重)]×100;
肌胃率(%)=(肌胃重/宰前活重)×100;
腺胃率(%)=(腺胃重/宰前活重)×100。
1.5.3 肠道发育
相对重量(g/kg)=肠段重/宰前活重;
单位长度重(g/cm)=肠段重/肠段长度。
1.5.4 肠道健康评分
1.5.5 盲肠肠道菌群
1.5.6 经济效益
每只鸡饲料成本(元/只)=平均采食量(kg/只)×饲料单价(元/kg);
增重饲料成本(元/kg)=采食量(kg)×饲料单价(元/kg)/增重(kg)。
1.6 数据处理与统计分析
2 结果与分析
2.1 粗糙米或糙米部分替代玉米对肉鸡生长性能的影响
表3 粗糙米或糙米部分替代玉米对肉鸡生长性能的影响Table 3 Effects of partial replacement of corn with partially husked rice or brown rice on growth performance of broilers |
| 项目 Items | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | SEM | P值 P-value |
|---|---|---|---|---|---|
| 12~24日龄12 to 24 days of age | |||||
| 12日龄体重BW at 12 days of age/g | 379.01 | 377.26 | 381.40 | 1.486 | 0.537 |
| 平均日增重ADG/(g/d) | 72.74 | 70.58 | 71.68 | 0.646 | 0.408 |
| 料重比F/G | 1.31 | 1.33 | 1.31 | 0.008 | 0.485 |
| 平均日采食量ADFI/(g/d) | 95.29 | 94.14 | 94.04 | 0.553 | 0.606 |
| 24日龄体重BW at 24 days of age/g | 1 324.64 | 1 294.82 | 1 313.30 | 9.243 | 0.428 |
| 25~38日龄25 to 38 days of age | |||||
| 平均日增重ADG/(g/d) | 87.92 | 89.35 | 92.13 | 1.272 | 0.406 |
| 料重比F/G | 1.74 | 1.72 | 1.69 | 0.010 | 0.204 |
| 平均日采食量ADFI/(g/d) | 152.76 | 153.95 | 155.82 | 1.891 | 0.794 |
| 38日龄体重BW at 38 days of age/g | 2 566.12 | 2 545.70 | 2 603.08 | 25.219 | 0.638 |
| 12~38日龄12 to 38 days of age | |||||
| 平均日增重ADG/(g/d) | 81.00 | 80.31 | 82.28 | 0.924 | 0.675 |
| 料重比F/G | 1.54 | 1.56 | 1.53 | 0.009 | 0.389 |
| 平均日采食量ADFI/(g/d) | 124.93 | 125.15 | 126.07 | 1.475 | 0.775 |
同行数据肩标无字母或相同字母表示差异不显著(P>0.05),不同小写字母表示差异显著(P<0.05)。下表同。 | |
In the same row, values with no letter or the same letter superscripts mean no significant difference (P>0.05), while with different small letter superscripts mean significant difference (P<0.05). The same as below. |
2.2 粗糙米或糙米部分替代玉米对肉鸡屠宰性能的影响
表4 粗糙米或糙米部分替代玉米对肉鸡屠宰性能的影响Table 4 Effects of partial replacement of corn with partially husked rice or brown rice on slaughtering performance of broilers % |
| 项目 Items | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | SEM | P值 P-value |
|---|---|---|---|---|---|
| 屠宰率Dressed percentage | 92.77 | 92.29 | 92.66 | 0.306 | 0.813 |
| 全净膛率Eviscerated percentage | 71.19 | 70.56 | 71.19 | 0.448 | 0.819 |
| 胸肌率Breast muscle percentage | 30.67 | 29.52 | 30.73 | 0.417 | 0.430 |
| 腿比率Thigh percentage | 32.28 | 32.75 | 31.99 | 0.287 | 0.574 |
| 翅膀率Wing percentage | 9.91 | 9.96 | 9.79 | 0.079 | 0.663 |
| 腹脂率Abdominal fat percentage | 1.34 | 1.37 | 1.25 | 0.074 | 0.792 |
| 肌胃率Gizzard percentage | 0.96 | 0.98 | 0.90 | 0.034 | 0.609 |
| 腺胃率Proventriculus percentage | 0.52 | 0.56 | 0.59 | 0.023 | 0.523 |
2.3 粗糙米或糙米部分替代玉米对肉鸡肠道发育与肠道健康评分的影响
表5 粗糙米或糙米部分替代玉米对肉鸡肠道发育的影响Table 5 Effects of partial replacement of corn with partially husked rice or brown rice on intestinal development of broilers |
| 项目 Items | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | SEM | P值 P-value |
|---|---|---|---|---|---|
| 相对重量Relative weigh/(g/kg) | |||||
| 十二指肠Duodenum | 7.61b | 9.27a | 7.78b | 0.292 | 0.030 |
| 空肠Jejunum | 14.43 | 14.72 | 16.16 | 0.518 | 0.362 |
| 回肠Ileum | 12.45 | 11.80 | 12.23 | 0.401 | 0.811 |
| 单位长度重Weight of unit length/(g/cm) | |||||
| 十二指肠Duodenum | 0.57 | 0.64 | 0.59 | 0.015 | 0.149 |
| 空肠Jejunum | 0.37b | 0.41ab | 0.42a | 0.010 | 0.034 |
| 回肠Ileum | 0.32 | 0.31 | 0.34 | 0.007 | 0.221 |
表6 粗糙米或糙米部分替代玉米对肉鸡肠道健康评分的影响Table 6 Effects of partial replacement of corn with partially husked rice or brown rice on intestinal health score of broilers |
| 项目 Items | 分值 Score | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | P值 P-value |
|---|---|---|---|---|---|
胀气 Flatulence | 0 | 5 | 7 | 6 | 0.501 |
| 1 | 3 | 1 | 2 | ||
| 2 | 0 | 0 | 0 | ||
水分过多 Excessive intestinal fluid | 0 | 1 | 2 | 3 | 0.327 |
| 1 | 2 | 3 | 4 | ||
| 2 | 5 | 3 | 1 | ||
肠壁变薄 Thinning of intestinal wall | 0 | 0 | 0 | 0 | 0.844 |
| 1 | 3 | 4 | 4 | ||
| 2 | 5 | 4 | 4 | ||
弹性减弱 Lack of tonus | 0 | 0 | 0 | 0 | 0.844 |
| 1 | 3 | 4 | 4 | ||
| 2 | 5 | 4 | 4 | ||
黏液过多 Excessive intestinal mucus | 0 | 0 | 1 | 0 | 1.000 |
| 1 | 4 | 5 | 4 | ||
| 2 | 4 | 2 | 4 | ||
黏膜脱落 Intestinal mucosa sloughing | 0 | 0 | 0 | 0 | 0.844 |
| 1 | 3 | 4 | 4 | ||
| 2 | 5 | 4 | 4 | ||
后肠段含料量 Undigested feed in the hindgut | 0 | 8 | 8 | 8 | 1.000 |
| 1 | 0 | 0 | 0 | ||
| 2 | 0 | 0 | 0 | ||
出血性肠炎 Hemorrhagic enteritis | 0 | 8 | 8 | 8 | 1.000 |
| 1 | 0 | 0 | 0 | ||
| 2 | 0 | 0 | 0 | ||
气泡增多 Excessive foamy intestinal contents | 0 | 6 | 8 | 8 | 0.077 |
| 1 | 2 | 0 | 0 | ||
| 2 | 0 | 0 | 0 |
表中列出的数据是每组各个评分所出现的次数。 The table listed the frequency of each score for each group. |
2.4 粗糙米或糙米部分替代玉米对肉鸡肠道菌群结构的影响
2.4.1 α-多样性分析
2.4.2 β-多样性分析
2.4.3 门和属水平菌群分析
表7 粗糙米或糙米部分替代玉米对肉鸡肠道菌群门水平相对丰度的影响Table 7 Effects of partial replacement of corn with partially husked rice or brown rice on relative abundances of intestinal microbes at phylum level of broilers % |
| 项目 Items | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | SEM | P值 P-value |
|---|---|---|---|---|---|
| 拟杆菌门Bacteroidetes | 62.20a | 41.14b | 37.20b | 2.634 | <0.001 |
| 厚壁菌门Firmicutes | 33.35b | 52.21a | 57.02a | 2.527 | <0.001 |
| 放线菌门Actinobacteria | 1.40 | 3.97 | 2.19 | 0.532 | 0.128 |
| 变形菌门Proteobacteria | 2.21 | 1.33 | 1.96 | 0.364 | 0.616 |
| 疣微菌门Verrucomicrobia | 0.06 | 0.01 | 0.02 | 0.013 | 0.245 |
表8 粗糙米或糙米部分替代玉米对肉鸡肠道菌群属水平相对丰度的影响Table 8 Effects of partial replacement of corn with partially husked rice or brown rice on relative abundances of intestinal microbes at genus level of broilers % |
| 项目 Items | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | SEM | P值 P-value |
|---|---|---|---|---|---|
| 拟杆菌属Bacteroides | 34.02a | 20.52b | 13.09b | 2.922 | 0.006 |
| 肠巴氏杆菌属Barnesiella | 1.74 | 1.27 | 3.22 | 0.505 | 0.270 |
| 粪杆菌属Faecalibacterium | 3.08 | 6.96 | 5.93 | 1.006 | 0.275 |
| 颤螺旋菌属Oscillospira | 1.65b | 4.77a | 6.97a | 0.627 | 0.001 |
| 乳杆菌属Lactobacillus | 4.37 | 3.66 | 6.07 | 0.839 | 0.504 |
2.4.4 菌群代谢通路
表9 具有显著差异的KEGG代谢通路(2级)Table 9 KEGG metabolic pathways with significant differences (level 2) |
| 项目 Items | 对照组 CON group | 粗糙米组 CCM group | 糙米组 CM group | SEM | P值 P-value |
|---|---|---|---|---|---|
| 细胞活动Cell motility | 382.16b | 480.38ab | 651.53a | 41.584 | 0.020 |
| 折叠、分类和降解 Folding, sorting and degradation | 1 242.32a | 1 220.73b | 1 195.51c | 5.551 | 0.001 |
| 心血管疾病Cardiovascular diseases | 0.00b | 0.02a | 0.01ab | 0.003 | 0.036 |
| 耐药性Drug resistance | 49.05b | 68.22a | 65.31a | 2.938 | 0.009 |
| 萜类化合物和多酮类化合物的代谢 Metabolism of terpenoids and polyketides | 3 234.10b | 3 279.24b | 3 460.93a | 39.189 | 0.036 |
| 内分泌系统Endocrine system | 26.27b | 29.05a | 29.39a | 0.567 | 0.040 |
2.5 粗糙米或糙米部分替代玉米对肉鸡经济效益的影响
表10 粗糙米或糙米部分替代玉米对肉鸡经济效益的影响Table 10 Effects of partial replacement of corn with partially husked rice or brown rice on economic benefits of broilers |
| 项目Items | 对照组CON group | 粗糙米组CCM group | 糙米组CM group |
|---|---|---|---|
| 每只鸡饲料成本Feed cost per chicken/(元/只) | 13.78 | 13.56 | 13.86 |
| 每千克增重饲料成本 Feed cost per kilogram weight gain/(元/kg) | 6.30 | 6.25 | 6.24 |

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