1 材料与方法
1.1 伦理声明
1.2 试验设计
表1 试验饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of experimental diets (air-dry basis)% |
| 项目 Items | 含量 Content | |||||
|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | ||
| 原料 Ingredients | ||||||
| 玉米 Corn | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | |
| 豆粕 Soybean meal | 10.7 | 3.5 | 3.5 | 3.5 | 3.5 | |
| 麦麸 Wheat bran | 8.0 | 12.7 | 12.5 | 12.3 | 12.1 | |
| 大豆油 Soybean oil | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 | |
| 大麦皮 Barley husk | 12.0 | 12.0 | 12.0 | 12.0 | 12.0 | |
| 次粉 Wheat middlings | 8.0 | 8.0 | 8.0 | 8.0 | 8.0 | |
| 稻壳粉 Rice hull powder | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | |
| 艾杆粉 Wormwood stem powder | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | |
| 花生壳 Peanut shell | 6.0 | 8.5 | 8.5 | 8.5 | 8.5 | |
| 碱草粉 Alkali grass powder | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | |
| 黑啤酒糟 Black beer lees | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 | |
| 玉米胚芽粕 Corn germ meal | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | |
| 蛋氨酸 Methionine | 0.2 | 0.4 | 0.6 | |||
| 预混料 Premix1) | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | |
| 合计 Total | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | |
| 营养水平 Nutrient levels2) | ||||||
| 消化能 DE/(MJ/kg) | 10.24 | 9.91 | 9.89 | 9.87 | 9.85 | |
| 粗蛋白质 CP | 17.21 | 15.08 | 15.23 | 15.02 | 15.16 | |
| 粗脂肪 EE | 2.96 | 2.82 | 2.85 | 2.87 | 3.05 | |
| 粗纤维 CF | 15.30 | 15.38 | 15.82 | 15.46 | 15.81 | |
| 中性洗涤纤维 NDF | 32.30 | 35.46 | 35.35 | 35.31 | 35.29 | |
| 酸性洗涤纤维 ADF | 20.04 | 21.08 | 20.96 | 20.75 | 20.42 | |
| 酸性洗涤木质素 ADL | 4.95 | 4.83 | 4.54 | 5.00 | 4.68 | |
| 钙 Ca | 1.26 | 1.17 | 1.29 | 1.20 | 1.14 | |
| 磷 P | 0.75 | 0.75 | 0.75 | 0.76 | 0.74 | |
| 赖氨酸 Lys | 0.99 | 0.98 | 0.99 | 0.93 | 1.01 | |
| 蛋氨酸 Met | 0.19 | 0.19 | 0.39 | 0.59 | 0.79 | |
| 蛋氨酸+胱氨酸 Met+Cys | 0.33 | 0.31 | 0.50 | 0.70 | 0.89 | |
1)每千克预混料包含 One kg of premix contained the following:Fe 60 mg,Zn 60 mg,Cu 20 mg,Mn 30 mg,I 0.5 mg,Se 0.05 mg,VA 8 000 IU,VD3 1 500 IU,VE 60 mg,VK 1.5 mg,VB1 1.5 mg,VB2 10 mg,VB6 1.5 mg,NaCl 5 g,磷酸氢钙 CaHPO4 15 g,赖氨酸 Lys 1.5 g,胆碱 chloride 150 mg,烟酸 nicotinic acid 35 mg,泛酸 pantothenic acid 15 mg,蛋氨酸 Met 1.5 g,叶酸 folic acid 0.5 mg,生物素 biotin 0.5 mg,VB12 0.5 mg,其余为杂粕载体补足 the rest was miscellaneous meal carrier complement。 | |
2)消化能为参照《肉兔营养需要量》(NY/T 4049—2021)的计算值,其余为实测值。DE was a calculated value reference to Nutrient Requirements of Meat Rabbit (NY/T 4049—2021), while the others were measured values. |
1.3 饲养管理
1.4 测定指标及方法
1.4.1 饲粮营养成分
1.4.2 生长性能
1.4.3 屠宰性能
1.4.4 血清生化和抗氧化指标
1.4.5 肉品质
1.4.6 肌肉营养成分和氨基酸含量
1.5 数据处理与分析
2 结果
2.1 低蛋白质饲粮中添加蛋氨酸对肉兔生长性能的影响
表2 低蛋白质饲粮中添加蛋氨酸对肉兔生长性能的影响Table 2 Effects of methionine supplementation in low protein diets on growth performance of meat rabbits |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 初重 IBW/g | 907.17 | 902.90 | 902.86 | 893.50 | 916.75 | 9.350 6 | 0.962 7 |
| 末重 FBW/g | 2 436.50a | 2 291.00b | 2 413.50a | 2 365.50ab | 2 361.12ab | 16.290 3 | 0.039 9 |
| 平均日增重 ADG/(g/d) | 43.77a | 39.99b | 42.93a | 41.95ab | 41.23ab | 0.406 8 | 0.024 1 |
| 平均日采食量 ADFI/(g/d) | 133.11ab | 135.13a | 135.19a | 126.53b | 127.38b | 1.175 0 | 0.026 4 |
| 料重比 F/G | 3.04b | 3.43a | 3.14b | 3.01b | 3.09b | 0.039 6 | 0.003 1 |
| 腹泻率 Diarrhea rate/% | 16.25 | 13.75 | 8.75 | 17.50 | 18.75 | 1.993 5 | 0.553 6 |
| 死亡率 Mortality rate/% | 18.75 | 10.00 | 6.25 | 13.75 | 15.00 | 2.117 9 | 0.407 0 |
同行数据肩标不同小写字母表示差异显著(P<0.05),相同或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different small letter superscripts mean significant difference (P<0.05), while with the same or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.2 低蛋白质饲粮中添加蛋氨酸对肉兔屠宰性能的影响
表3 低蛋白质饲粮中添加蛋氨酸对肉兔屠宰性能的影响Table 3 Effects of methionine supplementation in low protein diets on slaughter performance of meat rabbits |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 宰前活重 Pre-slaughter body weight/g | 2 514.75 | 2 318.87 | 2 504.71 | 2 408.25 | 2 357.50 | 29.823 1 | 0.141 0 |
| 半净膛重 Half eviscerated weight/g | 1 372.28 | 1 247.45 | 1 339.35 | 1 288.05 | 1 290.62 | 18.062 1 | 0.208 3 |
| 全净膛重 All eviscerated weight/g | 1 260.23 | 1 148.18 | 1 235.90 | 1 187.10 | 1 183.60 | 17.403 1 | 0.260 3 |
| 半净膛率 Half eviscerated ratio/% | 54.58 | 53.79 | 53.43 | 53.39 | 54.69 | 0.201 3 | 0.096 9 |
| 全净膛率 All eviscerated ratio/% | 50.12 | 49.51 | 49.30 | 49.17 | 50.13 | 0.219 0 | 0.499 6 |
2.3 低蛋白质饲粮中添加蛋氨酸对肉兔脏器指数的影响
表4 低蛋白质饲粮中添加蛋氨酸对肉兔脏器指数的影响Table 4 Effects of methionine supplementation in low protein diets on organ indexes of meat rabbits |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 心脏重 Heart weight/g | 6.83 | 6.68 | 6.73 | 6.83 | 7.28 | 0.137 0 | 0.680 5 |
| 肝脏重 Liver weight/g | 88.00 | 77.02 | 79.15 | 77.68 | 80.56 | 1.589 3 | 0.184 2 |
| 脾脏重 Spleen weight/g | 2.08 | 2.28 | 2.13 | 2.51 | 1.78 | 0.090 7 | 0.133 3 |
| 胸腺重 Thymus weight/g | 5.96 | 6.06 | 5.36 | 6.78 | 7.21 | 0.273 0 | 0.225 9 |
| 肾脏重 Kidney weight/g | 17.21 | 15.55 | 16.88 | 16.42 | 19.17 | 0.215 8 | 0.215 8 |
| 心脏指数 Heart index/(g/kg) | 2.72 | 2.88 | 2.65 | 2.84 | 3.09 | 0.488 9 | 0.147 5 |
| 肝脏指数 Liver index/(g/kg) | 34.98 | 33.22 | 31.40 | 32.50 | 34.24 | 0.652 2 | 0.457 8 |
| 脾脏指数 Spleen index/(g/kg) | 0.83 | 0.98 | 0.83 | 1.01 | 0.75 | 0.039 0 | 0.162 1 |
| 胸腺指数 Thymus index/(g/kg) | 2.36 | 2.62 | 2.15 | 2.59 | 3.05 | 0.116 9 | 0.166 8 |
| 肾脏指数 Kidney index/(g/kg) | 6.88 | 6.68 | 6.65 | 6.87 | 8.23 | 0.498 9 | 0.184 9 |
2.4 低蛋白质饲粮中添加蛋氨酸对肉兔肉品质的影响
表5 低蛋白质饲粮中添加蛋氨酸对肉兔肉品质的影响Table 5 Effects of methionine supplementation in low protein diets on meat quality of meat rabbits |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 滴水损失 Drip loss ratio/% | 6.55 | 7.21 | 7.02 | 7.09 | 6.93 | 0.142 5 | 0.673 4 |
| 蒸煮损失 Cooking loss ratio/% | 35.73 | 36.64 | 35.74 | 36.80 | 36.92 | 0.291 7 | 0.540 3 |
| 剪切力 Shear force/kgf | 2.46 | 2.68 | 2.57 | 2.45 | 2.61 | 0.076 9 | 0.874 0 |
| pH45 min | 6.65 | 6.85 | 6.97 | 6.89 | 6.74 | 0.039 9 | 0.095 3 |
| pH24 h | 5.64 | 5.72 | 5.69 | 5.62 | 5.67 | 0.018 8 | 0.546 9 |
| 亮度 L* | 46.62 | 45.32 | 47.33 | 46.69 | 45.65 | 0.367 0 | 0.425 2 |
| 红度 a* | 9.71 | 8.56 | 9.68 | 10.25 | 10.50 | 0.395 3 | 0.599 9 |
| 黄度 b* | 8.88 | 9.27 | 9.06 | 7.68 | 8.47 | 0.208 8 | 0.119 1 |
2.5 低蛋白质饲粮中添加蛋氨酸对肉兔背腰最长肌营养成分和氨基酸含量的影响
表6 低蛋白质饲粮中添加蛋氨酸对肉兔背腰最长肌营养成分和氨基酸含量的影响Table 6 Effects of methionine supplementation in low protein diets on contents of nutrients and amino acids in longissimus dorsi of meat rabbits% |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 营养成分 Nutrients | |||||||
| 粗灰分 Ash | 1.17 | 1.18 | 1.11 | 1.13 | 1.12 | 0.014 7 | 0.443 0 |
| 干物质 DM | 25.51 | 26.12 | 26.43 | 25.86 | 25.41 | 0.187 5 | 0.403 9 |
| 粗蛋白质 CP | 21.66 | 22.86 | 23.03 | 22.28 | 21.91 | 0.228 9 | 0.256 5 |
| 粗脂肪 EE | 1.46 | 1.41 | 1.40 | 1.26 | 1.30 | 0.047 0 | 0.664 5 |
| 氨基酸 Amino acids | |||||||
| 天冬氨酸 Asp | 9.98 | 9.95 | 10.05 | 9.97 | 9.96 | 0.012 1 | 0.068 4 |
| 苏氨酸 Thr | 4.97b | 4.96b | 4.99b | 5.01b | 5.09a | 0.009 9 | <0.000 1 |
| 丝氨酸 Ser | 3.96 | 4.01 | 3.99 | 4.08 | 4.16 | 0.023 3 | 0.050 2 |
| 谷氨酸 Glu | 15.74 | 15.63 | 15.75 | 15.91 | 15.93 | 0.045 0 | 0.172 6 |
| 甘氨酸 Gly | 4.48 | 4.60 | 4.53 | 4.48 | 4.43 | 0.021 6 | 0.165 6 |
| 丙氨酸 Ala | 5.95b | 5.99ab | 5.96b | 5.95b | 6.01a | 0.007 4 | 0.027 7 |
| 胱氨酸 Cys | 0.77 | 0.85 | 0.86 | 0.87 | 0.86 | 0.015 1 | 0.243 6 |
| 缬氨酸 Val | 5.73a | 5.66ab | 5.69ab | 5.58bc | 5.48c | 0.023 3 | 0.001 2 |
| 蛋氨酸 Met | 2.13 | 2.19 | 2.24 | 2.24 | 2.21 | 0.030 0 | 0.775 7 |
| 异亮氨酸 Ile | 5.09a | 4.99ab | 5.03ab | 4.94b | 4.83c | 0.020 8 | 0.000 1 |
| 亮氨酸 Leu | 8.76 | 8.71 | 8.73 | 8.73 | 8.73 | 0.011 0 | 0.712 1 |
| 酪氨酸 Tyr | 4.52 | 4.58 | 4.50 | 4.54 | 4.64 | 0.016 9 | 0.059 9 |
| 苯丙氨酸 Phe | 4.31 | 4.29 | 4.28 | 4.29 | 4.29 | 0.007 2 | 0.913 0 |
| 赖氨酸 Lys | 9.73ab | 9.66b | 9.67b | 9.73ab | 9.78a | 0.012 2 | 0.011 2 |
| 组氨酸 His | 3.30a | 3.29a | 3.26ab | 3.16b | 3.16b | 0.020 7 | 0.045 6 |
| 精氨酸 Arg | 6.78a | 6.79a | 6.78a | 6.72b | 6.67b | 0.010 7 | 0.000 2 |
| 脯氨酸 Pro | 3.70 | 3.76 | 3.65 | 3.73 | 3.72 | 0.015 1 | 0.145 6 |
2.6 低蛋白质饲粮中添加蛋氨酸对肉兔血清生化指标的影响
表7 低蛋白质饲粮中添加蛋氨酸对肉兔血清生化指标的影响Table 7 Effects of methionine supplementation in low protein diets on serum biochemical indexes of meat rabbits |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 总蛋白 TP/(g/L) | 52.13 | 51.56 | 50.20 | 51.44 | 50.95 | 0.666 5 | 0.927 1 |
| 白蛋白 ALB/(g/L) | 35.35 | 35.30 | 34.16 | 35.23 | 33.81 | 0.219 8 | 0.057 2 |
| 球蛋白 GLOB/(g/L) | 16.78 | 16.26 | 16.04 | 16.21 | 17.13 | 0.593 5 | 0.979 5 |
| 尿素 UREA/(mmol/L) | 6.65 | 7.66 | 6.33 | 6.81 | 7.56 | 0.188 8 | 0.098 7 |
| 尿素氮 UN/(mg/dL) | 18.64ab | 21.62a | 16.88b | 19.08ab | 21.16a | 0.532 2 | 0.033 6 |
| 葡萄糖 GLU/(mmol/L) | 5.05 | 4.73 | 5.34 | 5.37 | 5.42 | 0.108 1 | 0.219 6 |
2.7 低蛋白质饲粮中添加蛋氨酸对肉兔血清抗氧化指标的影响
表8 低蛋白质饲粮中添加蛋氨酸对肉兔血清抗氧化指标的影响Table 8 Effects of methionine supplementation in low protein diets on serum antioxidant indexes of meat rabbits |
| 项目 Items | 组别 Groups | SEM | P值 P-value | ||||
|---|---|---|---|---|---|---|---|
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |||
| 总超氧化物歧化酶 T-SOD/(U/mL) | 398.30c | 424.12bc | 438.98ab | 435.05ab | 459.62a | 5.845 2 | 0.009 5 |
| 丙二醛 MDA/(μmol/L) | 2.21 | 2.44 | 1.97 | 2.04 | 1.57 | 0.178 8 | 0.639 6 |
| 还原型谷胱甘肽 GSH/(μmol/L) | 5.85 | 5.97 | 5.45 | 6.05 | 5.58 | 0.181 5 | 0.830 3 |
| 总抗氧化能力 T-AOC/(mmol/L) | 0.49 | 0.43 | 0.41 | 0.45 | 0.42 | 0.009 4 | 0.091 0 |

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