选择3.5~4.0月龄、遗传来源相似、体重(12±2) kg的贵州从江香猪、剑白香猪去势公猪各6头,采用无氮饲粮(NFD)法和NFD+5%酶解酪蛋白(EHC)法进行代谢试验,测定贵州香猪内源氮与内源氨基酸的代谢规律,探索贵州香猪的维持蛋白质和氨基酸需要量。研究显示,贵州香猪内源氮排泄量为0.212 g/d W0.75,内源氨基酸排泄量为1.463 g/d W0.75。结果表明,贵州香猪可消化粗蛋白质维持需要量(DCPm)为1.91 g/d W0.75,可消化氨基酸维持需要量为2.10 g/d W0.75,以Lys为100建立贵州香猪维持需要可消化氨基酸模型为:Lys 100、Thr 54、Val 188、Met 40、Ile 79、Leu 126、Phe 101、His 25、Arg 58、Trp 17。
An experiment on metabolism of endogenous nitrogen and endogenous amino acids was carried out to ascertain the maintenance requirements of protein and amino acids for Guizhou Xiang pigs breed. Six castrated Congjiang Xiang pigs and 6 castrated Jianbai Xiang pigs at the age of 3.5 to 4 months and body weight of (12±2) kg with the same genetic background were selected, and nitrogen free diet (NFD) and NFD+5% enzyme-hydrolyzed casin (EHC) methods were adopted in this study. It showed the metabolic rule of endogenous nitrogen was 0.212 g/d W0.75 and that of endogenous amino acids was 1.463 g/d W0.75. The results indicate that the maintenance requirement of digestible crude protein (DCP) is 1.91 g/d W0.75, and that of amino acid (AA) is 2.10 g/d W0.75 for Guizhou Xiang pigs. The AA model (Lys as 100) is: Thr 54, Val 188, Met 40, Ile 79, Leu 126, Phe 101, Lys 100, His 25, Arg 58 and Trp 17.[Chinese Journal of Animal Nutrition, 2010,22(6):1523-1528]