试验用大肠杆菌脂多糖（LPS）多次刺激断奶仔猪建立应激模型，研究α-酮戊二酸（AKG）对断奶仔猪的生长性能和血浆激素水平的影响，探讨AKG能否缓解仔猪应激反应。选用24头健康断奶仔猪（杜洛克×长白×大白，体重（7.25±0.23） kg），随机分成3个组（空白对照组、应激对照组和AKG组），每组8个重复，每个重复1头猪。2个对照组饲喂基础日粮+1%淀粉，AKG组饲喂基础日粮+1% AKG，在试验第10天、第12天、第14天和第16天分别在应激对照组和AKG组仔猪的腹膜注射80 μg/kg LPS。试验结果显示：(1）试验第1～9天，未注射LPS时，AKG组平均日增重分别比空白对照组和应激对照组提高30.0%（P<0.05）和31.1%（P<0.05），料重比降低12.4%（P<0.05）和12.8%（P<0.05）；试验第10～16天（注射LPS），应激对照组与空白对照组相比平均日增重降低28.8%（P<0.05），料重比升高16.8%（P<0.05），但AKG组的平均日增重和料重比与空白对照组相比无显著性差异（P>0.05）;(2）与空白对照组相比，试验第10天，首次LPS刺激后，应激对照组的血浆胰岛素样生长因子（IGF-Ⅰ）水平降低22.7%（P<0.05），而AKG组无显著变化（P>0.05）；试验第16天，多次LPS刺激后，与空白对照组比较，应激对照组的IGF-Ⅰ和胰岛素（INS）水平分别降低21.2%（P<0.05）和25.7%（P<0.05），而AKG组无显著变化（P>0.05）。结果表明，日粮中添加1% AKG能显著缓解LPS应激对杜×长×大断奶仔猪生长的负影响。

Constituting a stress model by repeatedly challenged with lipopolysaccharide (LPS) in weaned pigs, the experiment that was researched on effects of α-ketoglutaric acid (AKG) on growth performance and plasma hormones level was aimed at whether AKG could alleviate the reaction to stress in weaned pigs. Twentyfour healthy crossbred Duroc× Landrace×Largewhite weaned piglets with body weight of (7.25±0.23) kg were randomly allocated to 3 groups (control group, LPS control group and AKG group). Every group included 8 replicates and 1 piglet in per replicate. The control group and LPS control group were fed the basal ration adding with 1% amylum, while AKG group was fed the basal ration adding with 1% AKG. Piglets in LPS control and AKG group were injected intraperitoneally with 80 μg/kg LPS every other day (on day 10, 12, 14 and 16 of experiment). The results showed that: 1) from day 1 to day 9 (without LPS challenge), ADG in AKG group increased by 30.0% (P<0.05) and 31.1% (P<0.05) compared with control group and LPS control group, respectively; meanwhile, F/G decreased by 12.4% (P<0.05) and 12.8% (P<0.05); from day 10 to day 16 (with LPS challenge), ADG and F/G in LPS control group decreased by 28.8% (P<0.05) and increased by 16.8% (P<0.05) compared with control group, respectively; while, there was no significant difference between AKG group and control group on ADG and F/G (P>0.05); 2) in comparison with control group, insulinlike growth factor-Ⅰ (IGF-Ⅰ) of plasma in LPS control group decreased by 22.7% (P<0.05) after LPS challenged firstly (on day 10 of experiment), but no significant difference was found in AKG group and control group; after repeated challenge with LPS (on day 16 of experiment), IGF-Ⅰ and insulin of plasma in LPS group decreased by 21.2% (P<0.05)and 25.7% (P<0.05); meanwhile, these indexes had no significant difference between AKG group and control group (P>0.05). The results indicated that dietary supplementation with 1% AKG could significantly relieve the growth depression of weaned piglets challenged by LPS.