本试验旨在探讨黄芪多糖对罗非鱼血浆中非特异性免疫酶活力以及胃肠内分泌阳性细胞数量的影响。选用体重（47±5）g的罗非鱼共150尾，随机分为5组，每组3个重复，每个重复10尾鱼。对照组饲喂基础日粮，4个试验组分别饲喂在基础日粮中添加500、1 000、1 500、2 000 mg/kg黄芪多糖的试验日粮。分别于试验第10天、第20天和第30天心脏采血并分离血浆，测定血浆中酸性磷酸酶（ACP）、碱性磷酸酶(AKP)、总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）和溶菌酶（LSZ）活力。试验进行至第40天时，采用免疫组化抗体检测罗非鱼胃体和前肠内增殖细胞核抗原（PCNA）阳性细胞、诱导型一氧化氮合酶（iNOS）阳性细胞及生长抑素（SS）阳性细胞数量。结果表明：与对照组相比，各试验组ACP、AKP、T-SOD、CAT和LSZ的活力均有所提高，在第20天和第30天时，1 000 mg/kg组和1 500 mg/kg组有显著或极显著升高（P<0.05或P<0.01）。与对照组相比，各试验组PCNA阳性细胞数量有所增加，iNOS和SS阳性细胞数量有所减少。其中，1 000 mg/kg组和1 500 mg/kg组与对照组差异显著或极显著（P<0.05或P<0.01）。由此得出，日粮中添加黄芪多糖可不同程度地提高罗非鱼血浆ACP、AKP、T-SOD、CAT和LSZ的活力，同时能够促进胃体和前肠部PCNA阳性细胞数量的增加及iNOS、SS阳性细胞数量的减少，且以1 000和1 500 mg/kg添加量效果较好。

The effects of astragalus polysaccharide (APS) on nonspecific immune enzyme activities in plasma and the quantity of endocrine positive cells in stomach and foregut of tilapia were discussed in this paper. One hundred and fifty tilapia with body weight of (47±5) g were randomly divided into 5 groups with 3 replicates and 10 tilapia in each replicate. Four test groups were fed with experiment diets which constituted of basic diet supplemented with different levels of APS (500, 1 000, 1 500 and 2 000 mg/kg), while control group was only fed with basic diet. Tilapia blood was collected from the heart on the 10th day, 20th day and 30th day of the test and centrifuged to plasma. The activities of acid phosphatase (ACP), alkaline phosphatase (AKP), total superoxide dismutase (T-SOD), catalase (CAT), and lysozyme (LSZ) were measured. On the 40th day of the test, the quantity of proliferating cell nuclear antigen (PCNA) positive cells, inducible nitric oxide synthase (iNOS) positive cells and somatostatin (SS) positive cells in stomach and foregut was detected by immunohisto-chemistry antibody. Results showed that: compared with the control group, the activities of ACP, AKP, T-SOD, CAT and LSZ in 4 test groups were all increased, and 1 000 and 1 500 mg/kg groups were significantly or extremely significantly higher (P<0.05 or P<0.01) on the 20th day and 30th day. The results also showed that the quantity of PCNA positive cells in test groups was higher than that in control group, while the quantity of iNOS and SS positive cells was lower, and 1 000 and 1 500 mg/kg groups were significantly or extremely significantly different with control group (P<0.05 or P<0.01). In conclusion, the diet with APS could increase the ACP, AKP, T-SOD, CAT and LSZ activities and the quantity of PCNA positive cells, and decrease the quantity of iNOS and SS positive cells. The APS levels of 1 000 and 1 500 mg/kg had better effects.［Chinese Journal of Animal Nutrition, 2010，22（2）:401-409］