本研究采用短期静态人工瘤胃发酵的体外培养法，研究了8种复合酶对玉米-豆粕-青干草型底物瘤胃发酵的影响。复合酶由纤维素酶、木聚糖酶、酸性蛋白酶、中性蛋白酶和果胶酶组成。结果表明：添加复合酶可以改变瘤胃发酵模式，提高饲料的消化率；各组复合酶处理的产气量、氨态氮浓度、VFA浓度与对照组相比有所增加 (P<0.05)。根据发酵参数判断，最佳复合酶中纤维素酶、木聚糖酶、酸性蛋白酶、中性蛋白酶、果胶酶和稻壳粉（载体）所占比例分别为20%、5%、5%、2%、2%和66%。

An  in vitro system was conducted to investigate the effects of eight different levels of exogenous complex enzymes in a corn-soybean meal hay diet on rumen fermentation. Exogenous complex enzyme consisted of cellulase, xylanase, acid protease, neutral protease and pectinase. The results showed that gas production, ammonia-N (NH₃-N), volatile fatty acids (VFA) concentration in each treatment were significantly higher than those in control treatment ( P <0.05). It also showed that the rumen fermentation pattern was changed when adding exogenous complex enzyme, and gas production in vitro , NH^₃-N and VFA concentration and their degradability were improved. The best formulation of complex enzyme was determined according to the fermentation parameters, the proportion of cellulase, xylanase, acid protease, neutral protease, pectinase and rice hull meal were 20%, 5%, 5%, 2%, 2% and 66%, respectively.