本试验旨在研究谷氨酰胺、壳聚糖及其二者复合添加对断奶仔猪小肠二糖酶活性、小肠绒毛高度及粪便菌群的影响。选取21日龄平均体重为（5.27±0.13）kg的断奶仔猪192头，随机分为4个处理，每个处理3个重复，每个重复16头猪。试验期14 d，分21～28 d和29～35 d 2个阶段，各组分别饲喂基础日粮（对照组）、基础日粮+1.00%谷氨酰胺（谷氨酰胺组）、基础日粮+0.04%壳聚糖（壳聚糖组）、基础日粮+1.00%谷氨酰胺+0.04%壳聚糖（复合组）。结果表明：1）与对照组相比，复合组十二指肠乳糖酶、麦芽糖酶活性、空肠乳糖酶、蔗糖酶活性及回肠乳糖酶、蔗糖酶活性均显著升高(P<0.05)；2）小肠绒毛高度在壳聚糖组、谷氨酰胺组、复合组依次呈升高趋势，但各组之间无显著性差异（P>0.05）；3）复合组仔猪粪便中大肠杆菌、葡萄球菌数量降低，乳酸杆菌数量增加，但差异均不显著（P>0.05）。由此可知，同时添加1.00%谷氨酰胺和0.04%壳聚糖显著提高断奶仔猪小肠二糖酶的活性，增加绒毛高度，改善粪便菌群组成，且其效果均优于其他单独添加组。

This experiment was conducted to study the effects of glutamine (Gln) and chitosan (CS) on small intestine disaccharidase activity, intestine villus height and faecal microflora of weanling piglets. One hundred and ninetytwo 21-day-old weanling piglets with an average body weight of (5.27±0.13) kg were randomly allocated into 4 treatments with 3 replicates in each treatment and 16 piglets in each replicate. The experiment which lasted for 14 days included two phases: 21～28 d and 29～35 d. The treatments were basal diet (CT), basal diet＋1.00% glutamine (GlnT), basal diet＋0.4% chitosan (CST) and basal diet＋1.00% glutamine＋0.04% chitosan (GCT), respectively. Results were as followed: 1) compared with CT, duodenum lactase and maltase activities, jejunum lactase and sucrase activities, ileum lactase and sucrase activities were significantly increased in GCT (P<0.05); 2) the intestine villus height from CST, GlnT to GCT was increased, but the differences between treatments were not significant （P>0.05）; 3) the quantities of E.coli and Staphylococci in GCT decreased but those of Lactobacillus increased whereas the differences were not significant （P>0.05）. These results indicated that the addition of 1.00% glutamine and 0.04% chitosan significantly increased small intestine disaccharidase activities (P<0.05) and intestine villus height and improved faecal microflora, and the effects were better than single addition.