本试验旨在评定3种低聚糖（果寡糖、棉籽糖和木寡糖）对幼年藏獒肠道菌群、腹泻和粪便质量的影响。试验选用平均体重为（10.18±1.66） kg的幼年藏獒12只，随机分成4组，对照组饲喂基础日粮，试验组饲喂在基础日粮中添加0.3%低聚糖（分别为果寡糖、棉籽糖和木寡糖）的日粮，试验期42 d。收集试验0、21和42 d时幼藏獒粪便，进行粪便评分、微生物培养，测定腹泻指数、粪氨、干物质和pH。结果表明：与对照组相比，3种低聚糖都有提高粪便质量的趋势，其中木寡糖组在42 d时效果最好（P＜0.05）；在42 d时，3种低聚糖组总需氧菌数量和大肠杆菌数量及粪氨含量显著降低（P＜0.05）、乳酸杆菌数量显著增高（P＜0.05），其中果寡糖组的效果最明显；3种低聚糖有降低pH的趋势。由此可知，低聚糖能一定程度上促进幼年藏獒的菌群平衡、减少腹泻、提高粪便质量，并且果寡糖的添加效果最好。

Effects of dietary supplementation of fructooligosaccharide (FOS), raffinose (ROS), xyloligosaccharide (XOS) on intestinal flora, diarrhea index and fecal quality in tibetan mastiff puppies were evaluated in this study. Twelve tibetan mastiff puppies with the body weight of （10.18±1.66） kg were divided into four groups: control (basal diet), FOS (added with 0.3% FOS in basal diet), ROS (added with 0.3% ROS in basal diet), XOS (added with 0.3% XOS in basal diet).The faeces were collected on the day of 0, 21 and 42, and the fecal score, number of bacteria, diarrhea index, fecal ammonia, DM and pH were measured. The results showed that three oligosaccharides had the tendency to improve the fecal quality, the XOS group had significant effect on fecal quality on the day of 42 (P＜0.05). The count of total aerobic bacteria and Bacillus coli and the content of fecal ammonia in three oligosaccharide groups were significantly decreased (P＜0.05) and the count of Bacillus acidilactici in three oligosaccharide groups were significantly increased (P＜0.05) on the day of 42, respectively, and the FOS group had the best effect. Three oligosaccharides had the tendency to decrease the fecal pH. It was concluded that oligosaccharides could improve microflora balance, prevent diarrhea and improve fecal quality in some degree, and the FOS group had the most significant effect.［Chinese Journal of Animal Nutrition, ２００9，２1（5）:763-768］