实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

南瓜多糖对大肠杆菌感染小鼠肠道损伤的保护作用及其机制研究

  • 陈茉 ,
  • 白书宁 ,
  • 王行 ,
  • 吕浩 ,
  • 董娜
展开
  • 东北农业大学动物科学技术学院, 哈尔滨 150030
陈茉(2001-),女,内蒙古赤峰人,本科生,动物营养专业。E-mail:chenmo2420@163.com

收稿日期: 2021-04-27

  网络出版日期: 2021-12-16

基金资助

东北农业大学SIPT计划项目(202110224047X)

Protective Effects of Pumpkin Polysaccharide on Intestinal Injury of Mice Infected by Enterotoxigenic Escherichia coli and Its Mechanism

  • CHEN Mo ,
  • BAI Shuning ,
  • WANG Xing ,
  • LYU Hao ,
  • DONG Na
Expand
  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2021-04-27

  Online published: 2021-12-16

摘要

本试验旨在研究南瓜多糖(PP)对大肠杆菌(ETEC)感染小鼠肠道损伤的保护作用及其分子机制。选用40只无特定病原体(SPF)级雄性ICR小鼠,随机分为4组,分别为对照(C)组、ETEC组、PP组及ETEC+PP组,其中C组及ETEC组饲喂基础饲粮,PP组及PP+ETEC组饲喂试验饲粮。试验期共21 d,在第18~20天给小鼠灌服ETEC菌液以建立肠炎模型。试验测定各组小鼠疾病活动指数、脏器指数、血清生化指标、肝脏抗氧化指标、肠道与肝脏组织炎症因子的表达水平。结果表明:PP显著降低小鼠疾病活动指数(P<0.05),极显著降低了肝脏指数和脾脏指数(P<0.01),并抑制血清中谷丙转氨酶(ALT)及谷草转氨酶(AST)活性,但各组之间无显著性差异(P>0.05)。此外,PP极显著提高了小鼠血清中的过氧化氢酶(CAT)活性和总抗氧化能力(T-AOC)(P<0.01),其肝脏中的CAT活性和T-AOC相较于ETEC组无显著性差异(P>0.05)。PP显著降低小鼠肠道中的白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)的mRNA相对表达量(P<0.05),而对肿瘤坏死因子-α(TNF-α)的mRNA相对表达量无显著性影响(P>0.05);PP显著降低肝脏中IL-6、TNF-αIL-1β的mRNA相对表达量(P<0.05)。以上结果表明,PP可通过提高小鼠抗氧化机能及抑制炎性反应从而减轻由ETEC肠道炎症引起的机体损伤。

本文引用格式

陈茉 , 白书宁 , 王行 , 吕浩 , 董娜 . 南瓜多糖对大肠杆菌感染小鼠肠道损伤的保护作用及其机制研究[J]. 动物营养学报, 2021 , 33(12) : 7070 -7077 . DOI: 10.3969/j.issn.1006-267x.2021.12.047

Abstract

The objective of this study was to investigate the protective effects of pumpkin polysaccharide (PP) on enterotoxigenic Escherichia coli (ETEC) infection in mice and its molecular mechanism. Firstly, 40 SPF male ICR mice were randomly divided into 4 groups:control (C) group, ETEC group, PP group and ETEC+PP group, control group and ETEC group were fed a basal diet, and PP group and PP+ETEC group were fed an experimental diet. The experiment lasted for 21 days, and ETEC was administered to mice by gavage to establish the intestinal inflammation model on the 18th to 20th day of the trial period. The disease activity index, organ index, serum biochemical indexes, liver antioxidant activity, and the mRNA relative expression levels of inflammatory factors in intestinal and liver tissues were measured in each group. The results showed that PP significantly decreased the disease activity index (P<0.05) and liver index and spleen index of mice (P<0.01), and inhibited serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, but there was no significant difference between groups (P>0.05). In addition, PP significantly increased the catalase (CAT) activity and total antioxidant capacity (T-AOC) in serum (P<0.01), while the CAT activity and T-AOC in liver had no significant difference between ETEC+PP group and ETEC group (P>0.05). PP significantly reduced the mRNA relative expression levels of interleukin-6 (IL-6) and interleukin-1β (IL-1β) (P<0.05) in the intestinal tract of mice, while had no significant effect on the mRNA relative expression levels of tumor necrosis factor-α (TNF-α) (P>0.05); PP significantly reduced the mRNA relative expression levels of IL-6, TNF-α and IL-1β in the liver (P<0.05). The results indicate that PP can reduce the body damage caused by ETEC intestinal inflammation by improving antioxidant function and inhibiting inflammatory response.

参考文献

[1] WIJTTEN P J A,VAN DER MEULEN J,VERSTEGEN M W A.Intestinal barrier function and absorption in pigs after weaning:a review[J].British Journal of Nutrition,2011,105(7):967-981.  
[2] 齐田琪.致病性大肠杆菌对犊牛血液生化指标、抗氧化及肠组织形态的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2020. QI T Q.Effects of pathogenic E.coli on blood biochemical indexes,antioxidant capacity and intestinal morphology of newborn calves[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2020.(in Chinese)
[3] XIA L,DAI L,ZHU L Q,et al.Proteomic analysis of IPEC-J2 cells in response to coinfection by porcine transmissible gastroenteritis virus and enterotoxigenic Escherichia coli K88[J].Proteomics Clinical Applications,2017,11(11/12):1600137.
[4] LIN Q,FU Q Q,LI X,et al.Human β-defensin 118 attenuates Escherichia coli K88-induced inflammation and intestinal injury in mice[J].Probiotics and Antimicrobial Proteins,2021,13(2):586-597.  
[5] 解影,马勇,谢春艳,等.浒苔多糖对ETEC感染小鼠肠道黏膜损伤的保护作用及机制研究[J].中国饲料,2019(23):64-68. JIE Y,MA Y,XIE C Y,et al.The mechanism of Enteromorpha polysaccharides in protecting the intestinal barrier injury of mice infected with ETEC[J].China Feed,2019(23):64-68.(in Chinese)
[6] 包晓玮,李凤鸣,韩海霞,等.南瓜及刺山柑多糖对小鼠免疫功能的影响[J].新疆农业科学,2010,47(3):508-511. BAO X W,LI F M,HAN H X,et al.Effects of pumpkin polycose and capparis spinosa Linn polycose on immune function of mice[J].Xinjiang Agricultural Sciences,2010,47(3):508-511.(in Chinese)
[7] 常慧萍,陶令霞,夏铁骑.南瓜多糖对四氧嘧啶型糖尿病小鼠血糖和血脂的影响[J].食品科技,2008,33(6):246-249. CHANG H P,TAO L X,XIA T Q.Effects of pumpkin polysaccharides on blood glucose and lipids in diabetic model rats by alloxan[J].Food Science and Technology,2008,33(6):246-249.(in Chinese)
[8] 柳红,张静.不同南瓜多糖体外清除羟基自由基作用的研究[J].武汉植物学研究,2007,25(4):356-359. LIU H,ZHANG J.Study on the scavenging effect of different pumpkin polysaccharide on hydroxide free radicals in vitro[J].Journal of Wuhan Botanical Research,2007,25(4):356-359.(in Chinese)
[9] 孟志卿,王玲.南瓜多糖对糖尿病小鼠血糖值、SOD、MDA的影响[J].信阳师范学院学报(自然科学版),2010,23(1):75-78. MENG Z Q,WANG L.The effect of pumpkin polysaccharide on the blood sugar of mice[J].Journal of Xinyang Normal University (Natural Science Edition),2010,23(1):75-78.(in Chinese)
[10] SONG Y,YANG Y,ZHANG Y Y,et al.Effect of acetylation on antioxidant and cytoprotective activity of polysaccharides isolated from pumpkin (Cucurbita pepo,lady godiva)[J].Carbohydrate Polymers,2013,98(1):686-691.  
[11] 黄诗琦,韦雅妮,赵雨川,等.鸡血藤总黄酮对大肠杆菌败血症的治疗作用[J].中国畜牧兽医,2019,46(5):1541-1550. HUANG S Q,WEI Y N,ZHAO Y C,et al.Therapeutic effect of total flavonoid of Spatholobus suberectus dunn on Escherichia coli sepsis[J].China Animal Husbandry & Veterinary Medicine,2019,46(5):1541-1550.(in Chinese)
[12] 赵宁.产肠毒素性大肠杆菌感染小鼠致腹泻LncRNA表达谱的构建[D].硕士学位论文.银川:宁夏大学,2016. ZHAO N.LncRNA expression signatures in response to enterotoxigenic Escherichia coli infection in mice[D].Master's Thesis.Yinchuan:NingXia University,2016.(in Chinese)
[13] YU E,CHEN D W,YU B,et al.Manno-oligosaccharide attenuates inflammation and intestinal epithelium injury in weaned pigs upon enterotoxigenic Escherichia coli K88 challenge[J]. British Journal of Nutrition,2020:1-10.
[14] GONG Z H,LIU Q L,LIN L,et al.l-theanine prevents ETEC-induced liver damage by reducing intrinsic apoptotic response and inhibiting ERK1/2 and JNK1/2 signaling pathways[J].European Journal of Pharmacology,2018,818:184-190.
[15] 王晓杰,吾买尔江·牙合甫,阿丽米热·买买提,等.菊苣多糖对小鼠药物性肝损伤保护作用的筛选[J].现代畜牧兽医,2021(4):9-13. WANG X J,YAKUP OMAR,MAMAT ALMIRA,et al.Screening of the protective effect of Cichorium intybus L. polysaccharide on drug-induced liver injury[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2021(4):9-13.(in Chinese)
[16] 吴凡,田娟,喻丽娟,等.饲料糖脂比对养成中期吉富罗非鱼生长性能、体成分和血清生化指标的影响[J].动物营养学报,2020,32(12):5805-5815. WU F,TIAN J,YU L J,et al.Effects of dietary carbohydrate to lipid ratio on growth performance, body composition and serum biochemical indices of genetic improvement of farmed tilapia in growth mid-stage[J].Chinese Journal of Animal Nutrition,2020,32(12):5805-5815.(in Chinese)
[17] 李毅腾,王晓闻,何庆峰,等.苦瓜皂苷与南瓜多糖或苦荞黄酮联合作用改善2型糖尿大鼠脂代谢的研究[J].现代食品科技,2015,31(9):14-19,37. LI Y T,WANG X W,HE Q F,et al.Effect of bitter melon saponins with buckwheat flavones or pumpkin polysaccharides on glucose and lipid metabolism in type-2 diabetic rats[J].Modern Food Science & Technology,2015,31(9):14-19,37.(in Chinese)
[18] BAKAR E,ULUCAM E,CERKEZKAYABEKIR A,et al.Investigation of the effects of naringin on intestinal ischemia reperfusion model at the ultrastructural and biochemical level[J].Biomedicine & Pharmacotherapy,2019,109:345-350.
[19] 宋丽君.南瓜多糖对糖尿病大鼠血糖、血脂及氧化应激能力的影响[J].中国应用生理学杂志,2015,31(1):65-66,71. SONG L J.Effects of pumpkin polysaccharide on the blood glucose,blood lipid and oxidative stress in diabetes rat[J].Chinese Journal of Applied Physiology,2015,31(1):65-66,71.(in Chinese)
[20] 印遇龙,杨哲.天然植物替代饲用促生长抗生素的研究与展望[J].饲料工业,2020,41(24):1-7. YIN Y L,YANG Z.Research and prospect of natural plant substitute for antibiotic growth promoters in feed[J].Feed Industry,2020,41(24):1-7.(in Chinese)
[21] 张超.硒化枸杞多糖的制备、抗氧化及保肝作用研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2019. ZHANG C.Study of preparation,antioxidation and liver protection of selenizing Lycium barbarum polysaccharide[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2019.(in Chinese)
[22] 刘颖,梁盈,林亲录,等.南瓜多糖的提取及其抗氧化活性研究进展[J].食品与机械,2014,30(3):239-243. LIU Y,LIANG Y,LIN Q L,et al.Research progress on polysaccharide extracted and antioxidant activity of pumpkin[J].Food & Machinery,2014,30(3):239-243.(in Chinese)
[23] 李哲涛.苦瓜多糖、南瓜多糖抗肿瘤与抗氧化活性的研究[D].硕士学位论文.西安:陕西师范大学,2013. LI Z T.Study on anti-tumor and antioxidant activity of polysaccharides from balsam pear and pumpkin[D].Master's Thesis.Xi'an:Shaanxi Normal University,2013.(in Chinese)
[24] 李红宇,许丽.籽用南瓜果肉用作反刍动物饲料的探讨[J].饲料研究,2017(13):49-52. LI H Y,XU L.Discussion on seed pumpkin flesh used as ruminant feed[J].Feed Research,2017(13):49-52.(in Chinese)
文章导航

/