本文旨在研究固态发酵对复合蛋白质饲料(CPF)营养成分和养分利用效率的改善效果。将产朊假丝酵母、枯草芽孢杆菌和乳酸杆菌接种到CPF后发酵3 d,干燥制得发酵复合蛋白质饲料(FCPF)。在测定豆粕(SBM)、CPF、FCPF常规养分和氨基酸含量基础上进行消化代谢试验。选择体重为(24-36±1-78) kg的杜×长×大生长猪24头,随机分为4组,每组6个重复,每个重复1头猪,分别饲喂无氮日粮和以SBM、CPF及FCPF作为唯一蛋白质源配制的半纯合日粮,采用无氮日粮法测定SBM、CPF和FCPF对生长猪的蛋白质、能量消化利用率以及氨基酸回肠消化率。预试期4 d,正试期4 d。结果表明:固态发酵极显著提高了FCPF的粗蛋白质、真蛋白质、钙和总磷含量(P<0.01);除精氨酸外,氨基酸含量均有所提高。与CPF相比,FCPF蛋白质消化利用率和能量消化率均极显著升高(P<0.01);氨基酸表观消化率显著或极显著的升高(P<0.05或P<0.01);除Gly以外,FCPF各氨基酸真消化率均显著或极显著的升高(P<0.05或P<0.01)。FCPF大部分营养特性接近或优于SBM。由此可知,固态发酵可改善CPF的营养特性,提高其在20-50 kg生长猪上的养分消化利用率。
This study focused on the improvement of nutritional value of compound protein feed (CPF) through solid state fermentation. CPF was fermented with C.utilis, B.subtilis and Lactobacillus for 3 days, then was dried into fermented compound protein feed (FCPF). Digestion and metabolism experiments of soybean meal (SBM), CPF and FCPF were conducted on the basis of analysis of nutrients and amino acids content. Twenty-four Duroc×Landrance×Yorkshire growing pigs with body weight of (24-36±1-78) kg were randomly allocated into 4 groups with 6 replicates in each group and 1 pig in each replicate. Pigs in 4 groups were fed N-free diet and semi-purified diets with CPF, FCPF and SBM as only protein source, respectively. The experiment lasted for 4 days for adaptation, and 4 days for sampling. The digestibility and availability of protein and energy, and ileal digestibility of amino acids on growing pigs were determined by N-free diet method. The results showed that, solid state fermentation could significantly improve the crude protein, true protein, calcium and total phosphorus contents of FCPF (P<0.01); content of amino acids except arginine were improved. Compared with CPF, digestibility and availability of protein and digestibility of energy (P<0.01), apparent digestibility of amino acids (P<0.05 or P<0.01) and true digestibility of amino acids (except Gly) (P<0.05 or P<0.01) were significantly improved. Most nutritive property were closed to or better than SBM. In conclusion, solid state fermentation could significantly improve the nutritive property and nutrient digestibility and availability of FCPF on 20-50 kg growing pigs.