This experiment was conducted to study the effects of α-ketoglutaric acid (AKG) on energy metabolism in intestinal mucosa of weaned pigs chronically challenged with lipopolysaccharide (LPS). Twenty-four healthy crossbred Duroc×Landrace×Largewhite weaned piglets with (7.25 ± 0.23) kg BW were randomly allocated to 3 groups (control group, LPS group and AKG group). Each group included 8 replicates, 1 piglet in per replicate. The control and LPS group were fed the basal diet supplemented with 1% starch, while the AKG group was fed the basal diet supplemented with 1% AKG. Piglets in the LPS group and AKG group were injected intraperitoneally with 80 μg/kg LPS on day 10, 12, 14 and 16, whereas piglets in the control group were injected intraperitoneally with normal saline at the same dose. On day 17, the intestinal mucosa was collected after pigs were slaughtered under anesthesia. Then the mucosal samples were used to measure the levels of adenylate using HPLC method. The results showed that dietary supplementation of 1% AKG could significantly increase the levels of ADP in duodenal mucosa (P<0.05), significantly relieve the decline of the levels of ATP and total adenine nucleotide in duodenal mucosa and jejunal mucosa and improve the levels of energy charge in jejunal mucosa and ileal mucosa. The results indicated that dietary supplementation with 1% AKG could relieve the dysfunction of intestinal mucosal energy metabolism induced by LPS challenge, therefore exert protective effects on intestinal mucosal barrier.［Chinese Journal of Animal Nutrition, 2009，21(6):892-896］