The variation of digesta flow in duodenum, jejunum and ileum of layer-type cockerels fitted with T-shaped cannula was investigated to study the biological character of intestinal digesta, which would provide a reference for developing in vitro digestion method in poultry feed evaluation. A 3×6 factorial completely randomized design with 3 digesta collection day and 6 digesta collection time per day was adopted. Fifteen birds in 3 groups were selected from 35 duodenal, 32 jejunal and 33 ileal cannulated cockerels, respectively, and each group was allotted into 5 replicates with 3 birds per replicate. The digesta was collected for 1 h out of every 4 h on d 31, 34 and 37 of the experiment. The flow of intestinal digesta, digesta dry matter, intestinal fluid, and total protein were determined. The results showed that no significant difference was found between the body weight on day 1 before cannulation operation and that on day 25 after operation （P>0.05）. The percentage of cannulated cockerels in good health was about 80% on day 120 after operation. There was no significant difference of survival ratio in 3 groups of cannulated cockerels （P>0.05）. The flow per hour of digesta, digesta dry matter and intestinal fluid was reduced from duodenum to ileum. Collection time in day cycle significantly affected the flow of digesta, digesta dry matter, intestinal fluid and total protein （P<0.05）. The significant differences were found in average flow of digesta, digesta dry matter and intestinal fluid in duodenum, flow of digesta and intestinal fluid in jejunum, as well as total protein of jejunal fluid on different collection days （P<0.05）. In conclusion, the cannulated cockerels can recover after 25 days and survive for at least 4 months. On ad libitum diet and natural light, the flow of digesta, digesta dry matter, intestinal fluid and total protein are more in daytime than night, and fluctuate on different collection days.［Chinese Journal of Animal Nutrition, 2011, 23（5）：789 -798］
ZHANG Jianzhi,ZHAO Feng，ZHANG Hongfu，WANG Hongrong
. A Study on the Variation of Intestinal Digesta Flow in Layer-type Cockerels Fitted with T-shaped Cannula[J]. Chinese Journal of Animal Nutrition, 2011
, 23(05)
: 789
-798
.
DOI: 10.3969/j.issn.1006-267x.2011.05.012
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