This study was conducted to determine the effects of dietary oxidized fish oil (OFO) and the intervention of soybean isoflavones (SI) supplementation on growth performance, intestinal mucosal oxidation-related indicators, immune factors, and co-oxidation and immune-related factors in neonatal piglets, and to explore the effects of oxidative stress on growth and intestinal immune response in neonatal piglets. The neonatal piglets (n=96, 4 day old) were selected and divided into four groups. They were fed 5% fresh fish oil (FFO) (group Ⅰ), 5% FFO+50 mg/kg SI (group Ⅱ), 5% OFO (group Ⅲ), OFO+50 mg/kg SI (group Ⅳ). After 21 d of feeding, two piglets per replicate were slaughtered, and small intestines were sampled. The results were showed as follows: at the end of 21 d, body weight of the neonatal piglets in group Ⅲ was significantly decreased compared with that in group Ⅱ (P<0.05). From 1 to 21 d, daily weight gain of the neonatal piglets in group Ⅲ was significantly lower than that in group Ⅰ and group Ⅱ (P<0.05). Compared with group Ⅰ and group Ⅱ, the content of malondialdehyde (MDA) in the intestinal mucosa of piglets in group Ⅲ was increased by 90.89% (P<0.05) and 73.28% (P<0.05), respectively. SI supplementation significantly improved the activity of total superoxide dismutase (T-SOD) in intestinal mucosa (P<0.05). The OFO and SI produced a significant interaction effect on the activity of total anti-oxidation capability (T-AOC) in intestinal mucosa (P<0.01). The content of interleukin-2 (IL-2) in group Ⅲ was significantly higher than the that in group Ⅰ (P<0.01) and the contents of interleukin-8 (IL-8) (P<0.05) and interleukin-10 (IL-10) (P<0.01) in group Ⅲ were significantly lower than those in group Ⅰ(P<0.05), respectively. The OFO significantly increased the activity of inducible nitric oxide synthase (iNOS) (P<0.05) and the content of nuclear factor-κB (NF-κB) (P<0.01). Collectively, these results suggest that dietary OFO significantly reduces the antioxidant capacity and causes intestinal inflammation in piglets (4 to 24 d old). SI supplementation can alleviate intestinal damage of neonatal piglets.［Chinese Journal of Animal Nutrition, 2011, 23（5）：799 -806］
HUANG Lin，JIANG Zongyong，LIN Yingcai，ZHOU Guilian，ZHENG Chuntian，CHEN Fang
. Influence of Oxidized Fish Oil on Intestinal Mucosal Immune Response in Neonatal Piglets and the Use of Soybean Isoflavones as an Intervention Measure[J]. Chinese Journal of Animal Nutrition, 2011
, 23(05)
: 799
-806
.
DOI: 10.3969/j.issn.1006-267x.2011.05.013
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