A 21-day feeding trial was conducted to study the effects of glyphosate-tolerant soybean meal on the intestinal mucosal immunity in Arbor Acres (AA) broilers, and to evaluate the safety of glyphosate-tolerant soybean meal in broilers from intestinal mucosal immunity angle. Three hundred and sixty 1-day-old AA broilers were randomly divided into two groups, and fed the diets containing glyphosate-tolerant soybean meal and conventional soybean meal, respectively. They were slaughtered on d 21 for sampling. The intestinal intraepithelial lymphocytes (iIEL) amount in duodenum, jejunum and cecum of broilers were observed by H & E staining, and the contents of sIgA in jejunum chyme, and interleukin 2 (IL-2) and interleukin 6 (IL-6) in jejunum tissue of broilers were detected using the enzyme-linked immunosorbent assay (ELISA). The results showed as follows: the number of iIEL in different parts of intestine was not significantly different between the two groups (P>0.05); the sIgA content in jejunum chyme was not significantly different between the two groups (P>0.05), and the contents of IL-2 and IL-6 as well as IL-2/IL-6 in jejunum tissue were not significantly different between the two groups (P>0.05). In conclusion, during the experimental period, there were no adverse effects of glyphosate-tolerant soybean meal on the detected indices of intestinal mucosal immunity in broilers.［Chinese Journal of Animal Nutrition, 2011, 23（5）：836 -841］
TAN Jianzhuang，LIU Shasha，SUN Zhe，ZHANG Hongfu，LU Qingping,SAREN Na,ZHAO Feng,YANG Xiaoguang
. Effects of Glyphosate-tolerant Soybean Meal on Intestinal Mucosal Immunity in Broilers[J]. Chinese Journal of Animal Nutrition, 2011
, 23(05)
: 836
-841
.
DOI: 10.3969/j.issn.1006-267x.2011.05.018
［1］PADGETTE S R, TAYLOR N B, NIDA D L, et al. The composition of glyphosate-tolerant soybean seeds is equivalent to that of conventional soybeans[J]. The Journal of Nutrition, 1996, 126(3):702-716.
[2]MCCANN M C, LIU K S, TRUJILLO W A, et al. Glyphosate-tolerant soybeans remain compositionally equivalent to conventional soybeans (Glycine max L.) during three years of field testing[J]. Journal of Agriculture and Food Chemistry, 2005, 53(13):5331-5335.
[3]HAMMOND B C, VICINI J L, HARTNELL C F. The feeding value of soybeans fed to rats, chickens, catfish and dairy cattle is not altered by genetic incorporation of glyphosate tolerance[J]. The Journal of Nutrition, 1996, 126(3):717-727.
[4]CROMWELL G L, LINDEMANN M D, RANDOLPH J H, et al. Soybean meal from roundup ready or conventional soybeans in diets for growing-finishing swine[J]. Journal of Animal Science, 2002, 80:708-715.
[5]TAYLOR M, HARTNELL G, LUCAS D, et al. Comparison of broiler performance and carcass parameters when fed diets containing soybean meal produced from glyphosate-tolerant (MON 89788), control, or conventional reference soybeans[J]. Poultry Science, 2007, 86:2608-2614.
[6]BRAKE D G, EVENSON D P. A generational study of glyphosate-tolerant soybeans on mouse fetal, postnatal, pubertal and adult testicular development[J]. Food and Chemical Toxicology, 2004, 42:29-36.
[7]JENNINGS J C, KOLWYCK D C, KAYS S B, et al. Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal[J]. Journal of Animal Science, 2003, 81:1447-1455.
[8]ASH J, NOVAK C, SCHEIDELER S E. The fate of genetically modified protein from Roundup Ready soybeans in laying hens[J]. Poultry Science, 2003, 12:242-245.
[9]MACDONALD T T, MONTELEONE G. Immunity, inflammation, and allergy in the gut[J]. Science, 2005, 307:1920-1925.
[10]IIJIMA H, TAKAHASHI I, KIYONO H. Mucosal immune network in the gut for the control of infectious diseases[J]. Reviews in Medical Virology, 2001, 11:117-133.
[11]TAGUCHI T, AICHER W K, FUJIHASHI K, et al. Novel function for intestinal intraepithelial lymphocytes. Murine CD3+, γ/δTCR+ T cells produce IFN-γ and IL-5[J]. Journal of Immunology, 1991, 147(11):3736-3744.
[12]YANG H, FAN Y Y, FINALY R, et al. Alteration of intestinal intraepithelial lymphocytes after massive small bowel resection[J]. Journal of Surgical Research, 2003, 110:276-286.
[13]杨倩，练高建，黄国庆.半胱胺对鸡小肠黏膜中分泌型IgA细胞和上皮内淋巴细胞的影响[J].南京农业大学学报,2002,25(2):89-92.
[14]杨玉荣，佘锐萍，张日俊.大豆生物活性肽对肉鸡肠道黏膜上皮内淋巴细胞和IgA+生成细胞的影响[J].中国预防兽医学报，2006,28(4):412-415.
[15]MESTECKY J R, MCGHEE J R. Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response[J]. Advances in Immunology, 1987, 40:153-245.
[16]ZHANG X F, ZHANG X W, YANG Q. Effect of compound mucosal immune adjuvant on mucosal and systemic immune responses in chicken orally vaccinated with attenuated Newcastle-disease vaccine[J]. Vaccine, 2007, 25:3254-3262.
[17]FINAMORE A, ROSELLI M, BRITTI S, et al. Intestinal and peripheral immune response to MON810 maize ingestion in weaning and old mice[J]. Journal of Agriculture and Food Chemistry, 2008, 56(23):11533-11539.
