The experiment was conducted to investigate the variation of sodium/glucose cotransporter 1 (SGLT1) and glucose transporter 2 (GLUT2) mRNA expression levels in small intestinal mucosa of weaning piglets and the influnence of dietary glutamine (GLN) on their expression. Sixty nine piglets weaned at 21-day-old were selected, 3 of them were slaughtered on the day of weaned, and the other 66 piglets were randomly divided into 2 groups with 3 replicates in each group and 11 heads per replicate. Piglets in control group and experimental group were fed basal diet and basal diet＋1% GLN, respectively. Three piglets from each group were slaughtered on the 3rd, 5th, 7th and 14th day after weaned, respectively. Mucosal tissues were collected from the different segments of small intestine (duodenum, jejunum and ileum) and the mRNA expressions of SGLT1 and GLUT2 were determined by real-time PCR. The results showed as follow: 1) There were no significant difference of SGLT1 and GLUT2 mRNA expression levels ameng different sigments of small intestine (P>0.05). 2) The SGLT1 mRNA expression levels in duodenum and jejunum decreased from the day of weaned, then increased from the 3rd day after weaned, reached the peak on the 14th day, while SGLT1 mRNA expression level in ileum showed an increase trend during the 0 to 5th day after weaned, then decreased on the 7th day, reached the peak on the 14th day; the GLUT2 expression levels in duodenum and jejunum showed increase trends during the 3rd to 7th day after weaned and reached the peak on the 7th day, then decreased to the same level as the weaned day on 14th day, while that of ileum reached the peak on the 5th day after weaned, then decreased to the same level as the weaned day. 3) Dietary supplemented with 1% GLN had no significant effect on expression levels of SGLT1 and GLUT2 in weaning piglets. The results indicate that the difference of of SGLT1 and GLUT2 mRNA expression levels in different sigments of small intestine are not significant, but the expression levels change with changing of time after weaned. The effects of GLN on SGLT1 and GLUT2 are not on the transcriptional level.［Chinese Journal of Animal Nutrition, 2011, 23（6）：983 -990］
ZHANG Shuai,LIU Tingting,ZHOU Lin,CHEN Anguo,HONG Qihua,YANG Caimei
. Variation of SGLT1 and GLUT2 mRNA Expression Levels in the Small Intestine of Weaning Piglets and the Influence of Dietary Glutamine[J]. Chinese Journal of Animal Nutrition, 2011
, 23(06)
: 983
-990
.
DOI: 10.3969/j.issn.1006-267x.2011.06.015
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