Rapeseed meal is an excellent resource of vegetable protein, whose detoxication and application have acquired extensive attention. Aspergillas niger is one of the microbes to detoxicate rapeseed meal. It can decrease the contents of phytin acid and glucosinolate in rapeseed meal effectively. The aim of the study is to establish an analytical method for the determination of glucosinolate in rapeseed meal with myrosinase. In this study, the myrosinase is extracted from selected Aspergillus niger H23 with glucosinolate-decomposing function via ultrasonication. It is the first time that microbial enzymes are used in the quantitative determination of glucosinolate. The process is simple and standard. The stability analysis shows that the method is much more stable than that of the endogenous myrosinase method. The study provides a new concept of glucosinolate analysis and a new way for the application of microbial myrosinase.［Chinese Journal of Animal Nutrition, 2011, 23（6）：1006 -1010］
LEI Lei,GAO Xuehua,DENG Xiaochen
. Determination of the Glucosinolate Content in Rapeseed Meal Using a Microbial Enzyme Method[J]. Chinese Journal of Animal Nutrition, 2011
, 23(06)
: 1006
-1010
.
DOI: 10.3969/j.issn.1006-267x.2011.06.018
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