This trial was conducted to assay the net energy (NE) of corn, soybean meal, rapeseed meal, cottonseed meal, wheat bran and rice bran for yellow-feather broilers aged from 1 to 21 days using the comparative slaughter method combined with regression and substitution methods, and to verify the accuracy of NE values and true digestible Lys (TDLys) requirements （NE 9.77 MJ/kg，TDLys 1.11%，absolute dry basis） which were suggested by YU with feeding experiment. NE values of feedsuffs were measured as the sum of NE for maintenance (NEm) and NE for deposition (NEp). NEm and NEp were determined by regression method and substitution method, respectively. In the feeding experiment, a total of 360 male broilers with an average weight of (38.8±2.3) g were randomly allocated to 6 groups (corn-soybean meal group, wheat bran group, rice bran group, rapeseed meal group, cottonseed meal group and mixture group ) with 6 replicates per group and 10 chicks per replicate, and the experiment lasted for 21 days. The average daily gain (ADG), average daily feed intake (ADFI), feed/gain ratio (F/G) and nitrogen deposition were calculated at the end of the trial. The results showed as follows: the NE values of corn, soybean meal, wheat bran, rice bran, rapeseed meal and cottonseed meal were 11.49, 7.62, 5.20, 9.86, 4.90, 5.22 MJ/kg DM, respectively. In the feeding experiment, F/G had no significant difference in the 6 groups (P>0.05); the ADG and ADFI in each group had no significant difference with those in corn-soybean meal group （P>0.05）, except for the mixture group; nitrogen deposition content in cottonseed meal group was higher than those in other groups (P<0.05), but there were no difference among the other groups (P＞0.05). In conclusion, the NE values measured by comparative slaughter method combined with regression and substitution methods are exact values, and the formulated diet in which NE and TDLys requirements were suggested by YU can achieve the same performance.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1094 -1100］