The present experiment was conducted to study the effects of Lactobacillus fermentum (L. fermentum) F-6 supplementation on enzyme activities in pancreas and small intestine and nutrient digestibility in broilers. In the experiment, a total of 240 one-day-old AA broilers (half male, half famale) with similar body weight were randomly allotted to 4 treatments with 6 replicates in each treatment, and with 10 broilers in each replicate. The trial lasted for 42 days. The basal diet was mainly composed of corn and soybean meal. Broilers were fed four levels (0, 2×105, 2×106 and 1×107 CFU/mL, respectively) of L. fermentum F-6 in drinking water in a single factorial arrangement. The results showed as follows: 1) the L. fermentum F-6 supplementation enhanced the activities of amylase in jejunum and pancreas, trypsin in jejunum and ileum, and chymotrypsin in duodenum of broilers (P<0.05), and the addition of 2×106 CFU/mL to the drinking water had the best effect. Compared to the control group (0 CFU/mL group), amylase activity in jejunum and pancreas was increased by 73.34% and 65.42%, respectively, and trypsin activity in jejunum and ileum was increased by 41.61% and 33.43%, respectively, and chymotrypsin activity in duodenum of broilers in 2×106 CFU/mL group was increased by 56.08% (P<0.05). However, there were no significant effects of L. fermentum F-6 supplementation on the activities of amylase in duodenum and ileum, trypsin in duodenum and pancreatic, and lipase in pancreas and small intestine were not significant (P>0.05). 2) L. fermentum F-6 supplementation could significantly improve the apparent digestibility of energy and protein in ileum of broilers (P<0.05), and the positive effects of 2×106 CFU/mL L. fermentum F-6 were the best. In conclusion, the L. fermentum F-6 supplementation can improved the digestive function of broilers, the effects are related to the levels of L. fermentum supplementation, and 2×106 CFU/mL L. fermentum F-6 shows the best pestive effects.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1225 -1232］
GUO Yuansheng,YAN Sumei,ZHANG Heping,FU Guohua,SHI Binlin
. Effects of Lactobacillus fermentum Supplementation on Enzyme Activities in Pancreas and Small Intestine and
Nutrient Digestibility in Broilers[J]. Chinese Journal of Animal Nutrition, 2011
, 23(07)
: 1225
-1232
.
DOI: 10.3969/j.issn.1006-267x.2011.07.022
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