This experiment was conducted to evaluate the nutritive value of carbonyl-amino urea for ruminants. The slow release characteristic of carbonyl-amino urea was evaluated in vitro with the change of ammonia N concentration. After that, an experiment with a 3×3 Latin Square design was carried out to study the effect of carbonyl-amino urea (CAU) on ruminal fluid pH, ammonia N concentration, microbial protein production and blood indices in Tibetan sheep. The results in vitro showed that: ammonia N concentration in CAU group was significantly lower than that in urea phosphate (UP) group and greater than that in soybean meal (SBM) group before 8 h, and it reached the maximum value of 42.76 mg/dL at the 8th h in artificial rumen fermentation, which was 11.27% lower than that in UP group and 44.08% higher than that in SBM group. The results in vivo showed that: ruminal fluid pH in all groups increased firstly, then decreased; it reached the minimum value at the 6th h after feeding and there was no difference (P>0.05) between SBM and CAU groups at the 6th h; after 14 h, there were no significant differences among the three groups. The ammonia N concentration in CAU group was lower than that in UP group, and there was on difference between SBM and CAU groups at the 2nd h after feeding(P>0.05); at the 8th h, ammonia N concentrations in SBM and UP groups reached the minimum values, and there were on differences at other times. The average concentration of microbial crude protein was 17.25 mg/dL, which was 3.01% higher than that in UP group and 30.45% higher than that in SBM group. Serum biochemical indices, such as BUN, TP, ALB, GOT and GPT, were all in normal physiological range and there were no significant differences among the three groups(P>0.05). Collectively, the CAU supplementation could control the release speed of ammonia nitrogen and improve the fermentation in rumen, and promote microbial protein production by improving the BUN availability for rumen microorganisms.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1239 -1246］
LI Xingwei,XUE Bai, WANG Zhisheng,XU Shixiao,WANG Jiheng,LI Zhanfeng
. Evaluation of the Nutritive Value of Carbonyl-amino Urea[J]. Chinese Journal of Animal Nutrition, 2011
, 23(07)
: 1239
-1246
.
DOI: 10.3969/j.issn.1006-267x.2011.07.024
［1］CHIZZOTTIF H M, PEREIRA O G, TEDESCHI L O, et al. Effects of dietary non-protein nitrogen on performance, digestibility, ruminal characteristics and microbial efficiency in crossbred steers[J]. Journal of Animal Science , 2008, 86(5):1173-1181.
[2]梁致远.纤维质材料的加氮作用研究——热分解和羰氨缩合[D].硕士学位论文．台北:国立台湾大学农业化学研究所,1991.
[3]HIGGNS J F, MCDONALD D R, HANSON M A, et al. Agriculturally useful, slow nitrogen-releasing products[J]. Chemical Abstract, 1973, 78:509-551.
[4]MENKE K H, RAAB L, SALEWSKI A, et al. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro[J]. Journal of Agricultural Science, 1979, 93:217-222.
[5]冯宗慈,高民.通过比色法测定瘤胃液氨态氮含量方法的改进[J].内蒙古农业科学,1993(4):40-41.
[6]ZINN R A, OWENS F N. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis[J]. Canadian Journal of Animal Science, 1986, 66: 157-166.
[7]LENG R A, NOLAN J V. Nitrogen metabolism in the rumen[J]. Journal of Dairy Science, 1984, 67(5):1072-1089.
[8]李静,张晓明,莫放,等.饲料级缩二脲作为反刍动物非蛋白氮饲料的安全性与有效性系列研究(2)[J].饲料研究,2007,12:54-57.
[9]胡红莲,卢德勋,刘大程,等.日粮不同NFC/NDF比对奶山羊瘤胃pH、挥发性脂肪酸及乳酸含量的影响[J].动物营养学报,2010,22(3):595-601.
[10]杨舒黎,王加启,胡志勇,等.日粮添加豆油和胡麻油对肉牛瘤胃发酵及主要微生物数量的影响[J].中国农业科学,2007,40(10):2316-2322.
[11]NOCEK J E. Feeding sequence and strategy effects on ruminal environment and production performance in first lactation cows[J]. Journal of Dairy science, 1992, 73(5):3100-3108.
[12]HART S P. Associative effects of sorghum silage and sorghum gram diets[J]. Journal of Animal Science, 1987, 64(6):1779-1789.
[13]谭正英,邵要伟,颜卉,等.不同尿素产品对奶牛采食和瘤胃发酵的影响[J].黑龙江畜牧兽医,2008,9:52-53.
[14]辛杭书,任丽萍,孙长勉,等.包被尿素与蒸汽压片玉米组合对活体外瘤胃氨氮释放和发酵参数的影响[J].中国农业大学学报,2007,12(3):41-45.
[15]CHURCH D C. The ruminant animal digestive physiology and nutrition[M]. Englewood: Waveland Press, 1988:108-116.
[16]周勤飞,王永才,王金勇,等.能量水平对生长猪生产性能、养分消化率和血清生化指标的影响[J].中国畜牧杂志,2010,46(23):44-47.
[17]KENNEDY P M, MILLIGAN L P. The degradation and utilization of endogenous urea in the gastrointestinal tract of ruminants[J]. Canadian Journal of Animal Science, 1980, 60:205-221.
[18]李建国,李英,曹玉,等.蛋白质补充料替代日粮中棉籽饼对肉牛生产性能和血液生化指标的影响[J].动物营养学报,2001,13(4):50-53.
