The study was conducted to investigate the effects of galactooligosaccharide (GOS) on fecal flora, blood indices and growth performance of Yanbian yellow calves. Twelve healthy newborn calves with similar birth weight were selected and randomly assigned into three groups by body weight and sex, with four heads in each group. Calves were drenched GOS at 0 (control group), 10 (low dose group, L group) and 15 g/d (high dose group, H group) from 2 to 29 days of age, respectively. Fecal samples were collected for the analysis of Escherichia coli and Bifidobacteria at 8, 15, 22 and 29 days of age; blood samples were collected for the analysis of physiological and biochemical indices at 15 and 29 days of age; birth weight and final weight were recorded. The results showed as follows: 1) Escherichia coli was the dominate flora in fecal in calves, the quantification of which steadily decreased with the calves’ growth, meanwhile, the quantification of Bifidobacteria increased and gradually became the dominate flora after 22-day-old; supplemental of GOS significantly increased the quantification of Bifidobacteria in fecal (P<0.05); 2) supplemental of GOS significantly increased the mean corpuscular hemoglobin concentration (MCHC) of calves at 29 days of age (P<0.05), but significantly decreased the number of red blood cell (RBC) (L group at 15 and 29 days of age, H group at 29 days of age, P<0.05) and the concentration of hemoglobin (HGB) in blood of calves (P<0.05); 3) the final weight and average daily gain in L group were significantly higher than those in control group (P<0.05). The results indicate that supplemental of GOS improves the balance of intestinal flora, and Bifidobacteria becomes the dominate flora in calves, increases the growth speed of calves, and the deficiency of hemopoiesis metrial becomes more serious, but the MCHC type anemia is relieved; the better level is 10 g/d under conditions in this study.[Chinese Journal of Animal Nutrition, 2011, 23(7):1247 -1252]
WANG Zhihang,JIANG Chengzhe,CUI Mingxun,AN Shangze,WANG Yan,JIANG He,LIU Huan,LI Honglong
. Effect of Galactooligosaccharide on Fecal Flora, Blood Indices and Growth Performance of Yanbian Yellow Calves[J]. Chinese Journal of Animal Nutrition, 2011
, 23(07)
: 1247
-1252
.
DOI: 10.3969/j.issn.1006-267x.2011.07.025
[1]TANAKA R, TAKAYAMA H. Effects of administration of TOS transgalactosylated oligosaccharide and Bifidobacterium breve 4006 on the human fecal flora[J]. Bifidobacteria and Microflora, 1983, 2(1):17-24.
[2]邰秀林,龙翔,向钊,等.低聚果糖对早期断奶犊牛生长性能和血液理化指标及肠黏膜形态的影响[J].中国畜牧杂志,2009(11):34-38.
[3]王喜明,许丽,孙文,等.低聚木糖对犊牛生长性能及粪便菌群的影响[J].中国畜牧杂志,2009(7):40-42.
[4]陈金春.微生物学实验指导[M].北京:清华大学出版社,2005.
[5]MOUNTZOURIS K C, MCCARTNEY A L, GIBSON G R. Intestinal microflora of human infants and current trends for its nutritional modulation[J]. British Journal of Nutrition, 2002, 87:405-420.
[6]ZECHEL D L, WITHERS S G. Glycosidase mechanisms: anatomy of a finely tuned catalyst[J]. Accounts of Chemical Research, 2000, 33(1):11-18.
[7]王镜岩,朱圣庚,徐长法.生物化学[M].3版.北京:高等教育出版社,2002.
[8]AMARETTI A, BERNARDI T, TAMBURINI E, et al. Kinetics and metabolism of Bifidobacterium adolescentis MB 239 growing on glucose, galactose, lactose, and galactooligosaccharides[J]. Applied and Environmental Microbiology, 2007, 73(11):3637-3644.
[9]ADAMCZAK M, CHARUBIN D, BEDNARSKI W. Influence of reaction mediu composition on enzymatic synthesis of galactooligosaccharides and lactulose from lactose concentrates prepared from whey permeate[J]. Chemical Papers, 2009, 63(2):111-116.
[10]GIBSON G R, ROBERFROID M B. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics[J]. Journal of Nutrition, 1995, 125:1401-1412.
[11]MOREAU M C, GABORIAU-ROUTHIAU V. Immunomodulation by the gut microflora and probiotics[J]. Probiotics, 2000, 3:69-114.
[12]SWENSON M J, REECE W O. Duckes physiology of domestic animal[M]. 11th ed. New York: Cornell University Press, 1993.
[13]BRUN-HANSEN H C, KAMPEN A H, LUND A, et al. Hematologic values in calves during the first 6 months of life[J]. Veterinary Clinical Pathology, 2006, 35(2):182-187.
[14]BOMBA A, SEVCIK A, POLDAUF M. Changes in erythrocytes, serum iron and serum copper in calves and after weaning, with reference to anaemia[J]. Veterinarstvi, 1986, 36:227-229.
[15]REECE W O, HOTCHKISS D K. Blood studies and performance among calves reared by different methods[J]. Journal of Dairy Science, 1987, 70(8):1601-1611.
[16]BAMI M H, MOHRI M, SEIFI H A, et al. Effects of parenteral supply of iron and copper on hematology, weight gain, and health in neonatal dairy calves[J]. Veterinary Research Communications, 2008, 32(7):553-561.