Fish Nutrition

Different Sources and Levels of Vitamin C in Jian Carp (Cyprinus carpio var. Jian): A Comparison of Their Effects on
Growth Performance and Digestion Function

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  • 1. Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China; 2. Sichuan Province Key & Open Lab, Ya’an 625014, China; 3. Chengdu Sunwind
    Agricaltural and Livestock Farming Co., Ltd., Chengdu 621430, China

Online published: 2011-08-15

Abstract

The experiment was conducted to study the effects of different sources and levels of vitamin C on growth performance and digestive function of Jian carp (Cyprinus carpio var. Jian). Six hundred Jian carp with initial average body weight of (12.63±0.04) g were randomly allocated to 4 groups (with 3 replicates per group and 50 fish per replicate). The Jian carp were fed with practice diets supplemented with different levels (75 or 150 mg/kg diet) of ethylcellulose-coated ascorbic acid (EC-AA) or L-ascorbyl polyphosphate (LAPP) for 8 weeks. When supplemented with 75 mg/kg vitamin C in diets, specific growth rate (SGR), feed intake (FI), protein production value (PPV) and lipid production value (LPV) of carp in LAPP group were all significantly higher than those in EC-AA group (P<0.05). Meanwhile, compared with the EC-AA diet, the LAPP diet could significantly improve the growth and development of hepatopancreas and intestine, and enhance the activities of tryspin, chymotrypsin and lipase in intestine, folds height in different intestine segments, activities of alkaline phosphatase (AKP) and glutamyl transpeptidase (γ-GT) in different intestine segments, and activities of Na+,K+-ATPase in proximal and distal intestine segments and creatine kinase (CK) in whole intestine (P<0.05). Whereas, when supplemented with 150 mg/kg vitamin C in diets, the SGR, FI, PPV and LPV were not significantly different between LAPP group and EC-AA group (P>0.05). However, compared with the EC-AA diet, the LAPP diet could significantly enhance the activities of tryspin and chymotrypsin in intestine, folds height in mid and distal intestine segments, activities of AKP in distal intestine, Na+,K+-ATPase in proximal intestine and γ-GT in different intestine segments (P<0.05). In conclusion, the effect of LAPP is better than EC-AA for Jian carp, and the LAPP supplementation of 75 mg/kg vitamin C can more significantly improve the growth performance and digestion function of Jian carp.[Chinese Journal of Animal Nutrition, 2011, 23(8):1332 -1341]

Cite this article

LIU Yang,CHI Lei,FENG Lin,ZHOU Xiaoqiu . Different Sources and Levels of Vitamin C in Jian Carp (Cyprinus carpio var. Jian): A Comparison of Their Effects on
Growth Performance and Digestion Function[J]. Chinese Journal of Animal Nutrition, 2011
, 23(08) : 1332 -1341 . DOI: 10.3969/j.issn.1006-267x.2011.08.012

References

[1]WILSON R P. Absence of ascorbic acid synthesis in channel catfish Ictalurus punctatus and blue catfish Ictalurus frucatus[J]. Comparative Biochemistry Physiology Part B, 1973, 46:635-638.

[2]艾庆辉,麦康森,王正丽,等.维生素C对鱼类营养生理和免疫作用的研究进展[J].水产学报,2005,29(6):857-861.

[3]SHIAU S Y, HSU T S. Stability of ascorbic acid in shrimp feed during analysis[J]. Nippon Suisan Gakkaishi, 1993, 59:1535-1537.

[4]朱健,王建新.建鲤品种特性研究进展[J].浙江海洋学院学报:自然科学版,2004,23:52-55.

[5]刘扬.饵料维生素C营养需要研究[D].硕士学位论文.雅安:四川农业大学,2001:1-60.

[6]王文辉,王吉桥,程鑫,等.不同剂型维生素C对黄颡鱼生长和几种免疫指标的影响[J].中国水产科学,2006,13(6):951-958.

[7]NRC. Nutrient requirements of fish[S]. Washington, D. C.: National Academy Press, 1993.

[8]赵玉娟,李如华,刘慧文.牛肉样品中维生素C的HPLC测定[J].现代仪器,2005(3):32-33.

[9]LIU P R, PLUMB J A, GUERIN M, et al. Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds[J]. Diseases of Aquatic Organisms, 1989, 7:191-194.

[10]AOAC. Official methods of analysis of the AOAC International[S]. 17th ed. Gaithersburg, MD: AOAC International, 2000.

[11]BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72:248-254.

[12]HUMMEL B C W. A modified spectrophotometric determinations of chymotrypsin, trypsin, and thrombin[J]. Canadian Journal of Biochemistry and Physiology, 1959, 37:1393-1399.

[13]FURNEA M, HIDALGOA M C,LOPEZ A.Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss.A comparative study[J]. Aquaculture, 2005, 250:391-398.

[14]REFSTIE S, LANDSVERK T, BAKKE-MCKELLEP  M, et al. Digestive capacity, intestinal morphology, and microflora of 1-year and 2-year old Atlantic cod (Gadus morhua) fed standard or bioprocessed soybean meal[J]. Aquaculture, 2006, 261:269-284.

[15]MCCORMIC S D. Methods for nonlethal gill biopsy and measurements of Na+,K+-ATPase[J]. Canadian Journal of Fisheries and Aquatic Science, 1993, 50:656-658.

[16]WILSON R P, POE W E, ROBINSON E H. Evaluation of L-ascorbyl-2-polyphosphate (AsPP) as a dietary ascorbic acid source for channel catfish[J]. Aquaculture, 1989, 81:129-136.

[17]冷向军,王道尊,李小勤.青鱼鱼种饲料中不同剂型维生素C适宜添加量的研究[J].四川农业大学学报,2002,20(2):141-143.

[18]林仕梅,罗莉,叶元土,等.不同剂型VC对草鱼生长的影响[J].粮食与饲料工业,2003,4:30-32.

[19]KUMULU M, JONES D A. The effect of live and artificial diets on growth, survival, and tripsin activity in larvae of Penaeus indicus[J]. Journal of the World Aquaculture Society, 1995, 26:406-415.

[20]PEDERSEN H E, SISSONS J W. Effect of antigenic soyabean protein on the physiology and morphology of the gut in the preruminant calf[J]. Canadian Journal of Animal Science, 1984, 64:183-184.

[21]FARHANGI M, CARTER C G, HARDY R W. Growth, physiological and immunological responses of rainbow trout (Oncorhynchus mykiss) to different dietary inclusion levels of dehulled lupin (Lupinus angustifolius)[J]. Aquaculture Research, 2001, 32:329-340.

[22]VILLANUEVA J, VANACORE R, GOICOECHEA O, et al. Intestinal alkaline phosphatase of the fish Cyprinus carpio: regional distribution and membrane association[J]. Journal of Experimental Zoology, 1997, 279:347-355.

[23]LIN Y, ZHOU X Q. Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J]. Aquaculture, 2006, 256:389-394.

[24]BELL J G, BUDDINGTON R K, WALTON M J, et al. Study on the putative role of gamma-glutamyl transpeptidase in intestinal transport of amino acids in Atlantic salmon[J]. Journal of Compare Physiology, 1987, 157(2):161-169.

[25]周秀菊,刘党生,蒋宇扬.肌酸激酶结构与功能研究进展[J].沈阳医科大学学报,2002,19(5):386.
 
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