The experiment was conducted to investigate the effect of dietary digestible energy (DE) levels on performance, lipid metabolism and lipoprotein lipase (LPL) gene expression in fat and muscle tissues of China Rongchang strain’s roast sucking pigs. A total of 72 healthy weanling piglets with average body weight of (4.95±0.78) kg were randomly allotted to 4 treatments with 3 replicates per treatment and 6 pigs per replicate. Piglets were fed with 4 formulated diets contained 13.39, 14.23, 15.06 and 15.90 MJ/kg DE, respectively. The experiment lasted for 28 d. For this experiment, these indices were determined such as performance, concentrations of lipids in serum, LPL and malate dehydrogenase (MDH) activity in longissimus dorsi (LM), and LPL mRNA expression in muscle and fat tissues. The results showed as follows: 1) there was a significant quadratic increase in average daily gain (ADG) as dietary DE level increased (P<0.05); the ratio of feed and gain (F/G) decreased linearly along with the increasing of dietary DE level (P<0.05). 2) The concentration of total triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) in serum increased linearly (P<0.01) as dietary DE level increased, however, there were no significant differences observed in total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and non-esterified fatty acids (NEFA) among the groups (P>0.05). 3) Increasing dietary DE level resulted in a linear increase of MDH activity in LM (P<0.01), but there was no effect detected in LPL activity (P>0.05); in addition, increasing dietary DE level linearly enhanced the expression of LPL mRNA in adipose tissues (P<0.01). 4) The expression of LPL mRNA in LM was not affected by the increasing of dietary DE level. The results indicate that dietary DE levels affect performance and blood lipid concentrations, modified the activities of enzymes involved in lipid metabolism and the expressions of related genes, then finally affect body fat deposition to some degree, and modified the meat quality of China Rongchang strain’s roast sucking pigs.［Chinese Journal of Animal Nutrition, 2011, 23（9）：1490 -1498］
ZENG Zhen,CHEN Daiwen,MAO Xiangbing,MAO Qian,YU Bing
. Effect of Digestible Energy Levels on Performance and Lipid Metabolism of China Rongchang Strain’s Roast Sucking Pigs[J]. Chinese Journal of Animal Nutrition, 2011
, 23(09)
: 1490
-1498
.
DOI: 10.3969/j.issn.1006-267x.2011.09.006
［1］王丽.饲粮能量水平和来源对育肥猪骨骼肌脂肪代谢相关基因表达的影响[D].硕士学位论文.雅安：四川农业大学，2009.
[2]刘作华，陈代文，龙定彪，等.日粮能量水平对猪脂肪酸合成酶和激素敏感酯酶的影响[J].中国畜牧杂志，2009，45(5)：31-33.
[3]陈德志，余冰，陈代文.日粮能量蛋白质水平对荣昌烤乳猪品系生长性能和肉质性状的影响[J].动物营养学报，2009，21(5)：634-639.
[4]刑启银.不同能量水平饲粮对生长育肥猪生长性能的影响[J].养猪，2006（1）:27-28.
[5]BEE G, GEBERT S, MESSIKOMMER R. Effect of dietary energy supply and fat source on the fatty acid pattern of adipose and lean tissues and lipogenesis in the pig[J]. Journal of Animal Science, 2002, 80:1564-1574.
[6]BEAULIEU A D, LEVESQUE C L, PATIENCE J F. The effects of dietary energy concentration and weaning site on weanling pig performance[J]. Journal of Animal Science, 2006, 84:1159-1168.
[7]SMITH J W, TOKACH M D, O′QUINN P R, et al. Effects of dietary energy density and lysine: calorie ratio on growth performance and carcass characteristics of growing-finishing pigs[J]. Journal of Animal Science, 1999, 77:3007-3015.
[8]DE LA LLATA M, DRITZ S S, TOKACH M D, et al. Effect of dietary fat on growth performance and carcass characteristics of growing-finishing pigs reared in a commercial environment[J]. Journal of Animal Science, 2001, 79:2643-2650.
[9]郑红霞.饲料消化能水平对CRP配套系水平代猪肉品质的影响[D].硕士学位论文.重庆：西南大学，2007.
[10]余德谦，林映才，蒋宗勇，等.生长肥育猪营养需求模式试验[J].养猪,2002(2):3-6.
[11]RAEINI-SARJAZ M, VANSTONE C A, PAPAMANDJARIS A A, et al. Comparison of the effect of dietary fat restriction with that of energy restriction on human lipid metabolism[J]. American Journal of Clinical Nutrition, 2001, 73(2):262-267.
[12]晁金.日粮能量浓度对肥育猪脂肪组织中脂肪沉积相关指标的影响[D].硕士学位论文.武汉：武汉工业大学，2009.
[13]GARAULET M, PEREZ-LLAMAS F, PEREZ-AYALA M, et al. Site-specific differences in the fatty acid composition of abdominal adipose tissue in an obese population from a Mediterranean area: relation with dietary fatty acids, plasma lipid profile, serum insulin, and central obesity[J]. American Journal of Clinical Nutrition, 2001, 74(5): 585-591.
[14]张传涛．特种野猪适宜能量、蛋白质需要量的研究[D]．硕士学位论文．南宁：广西大学，2008.
[15]MERSMAN H J, POND W G, YEN J T. Use of carbohydrate and fat as energy source by obese and lean swine[J]. Journal of Animal Science, 1984, 58:894-902.
[16]杨海玲，曾勇庆，魏述东，等.菜芜猪脂肪代谢酶活性的发育性变化及其对肌内脂肪沉积的影响[J].畜牧兽医学报，2005，36(11)：1150-1154.
[17]罗献梅.猪H-FABP基因的表达规律及营养水平和营养源对猪肉质和H-FABP基因表达的影响[D].博士学位论文.雅安：四川农业大学，2007.
[18]宋庆文．不同日粮营养水平下荣昌猪和DLY猪脂肪(酸)代谢关键酶活性的发育性变化研究[D]．硕士学位论文．呼和浩特：内蒙古农业大学，2007．
[19]FAULCONNIER Y, BONNET M, BOCQUIER F, et al. Effects of photo period and feeding level on adipose tissue and muscle lipoprotcin lipase activity and mRNA level in dry non-pregnant sheep[J]. British Journal of Nutrition, 2001, 85(3):299-306.
[20]SHAN T Z, WANG Y Z, LI M. Cloning of lipoprotein lipase (LPL) gene of swine and the difference of LPL gene expression at different avoirdupois stages[J]. Journal of Agriculture Biotechnology, 2006, 14(2):151-155.
[21]黄英俏，杨连玉.脂蛋白脂酶研究进展动物医学进展[J].2010，31(3)：86-89.
[22]王刚，曾勇庆，武英．猪肌肉组织LPL基因表达的发育性变化及其与肌内脂肪沉积关系的研究[J]．畜牧兽医学报，2007，38(3)：253-257．
[23]GERFAULT V, LOUVEAU I, MOUROT J, et al. Lipogenic enzyme activities in subcutaneous adipose tissue and skeletal muscle from neonatal pigs consuming maternal or formula milk[J]. Reproduction Nutrition Development, 2000, 40(2):103-112.
[24]VANNIER C, AMRI E Z, ETIENNE J, et al. Maturation and secretion of lipoprotein lipase in cultured adipose cells 1. Interacellular activation of the enzyme[J]. Journal of Biological Chemistry, 1985, 260:4424-4435.
[25]QIAO Y, HUANG Z G, LI Q F, et al. Developmental changes of the LPL mRNA expression and its effect on IMF content in sheep muscle[J]. Agricultural Sciences in China, 2008, 7(1):104-111.
[26]RANGANATHAN G, ONG J M, YUKHT A, et al. Tissue-specific expression of human lipoprotein lipase: effect of the 3’-untranslated region on translation[J]. The Journal of Biological Chemistry, 1995, 270:7149-7155.
[27]BONNET M, CHRISTINE L, CHILIARD Y, et al. Rapid communication: nucleotide sequence of the ovine lipoprotein lipase cDNA[J]. Journal of Animal Science, 2000, 78:2994-2995.
