The experiment was conducted to study the effect of dietary isomalto-oligosaccharide (IMO） on performance, caecal microflora, antioxidation and immune function of laying hens. Six hundred Hy-Line variety brown laying hens aged 200 days were randomly allotted into 5 groups, and each group was replicated 6 times, with 20 birds per replicate. Laying hens in the five groups were fed with the basal diet supplemented with 0, 0.1%, 0.2%, 0.4% and 0.8% of IMO, respectively. The experiment lasted for 8 weeks. The results showed as follows: compared with the control group, average egg weight, laying rate, egg mass, feed/egg ratio, albumen height and Haugh unit of laying hens in 0.1% and 0.2% IMO groups were improved to a certain extent. The laying performance and egg quality of laying hens in 0.8% IMO group were lower than those in 0.1% and 0.2% IMO groups. Globulin/albumin ratio (Glb/Alb) in plasma of laying hens in 0.2% and 0.4% IMO groups was increased (P<0.05), while urea nitrogen content was reduced (P<0.05). The number of Lactobacillus and Bifidobacterium in caecum of laying hens in 0.2% IMO group was increased (P<0.05), while that of E. coli was decreased (P<0.05). Compared with the control group, the activities of superoxidedismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidative capacity (T-AOC) in serum of laying hens were significantly increased by 0.2% and 0.4% IMO supplementation in diets (P<0.05). When 0.2% to 0.8% IMO was added in diets, the contents of IgA, IgM and IgG and lysozyme activity in plasma of laying hens were all increased (P<0.05). In conclusion, dietary IMO can improve the performance，optimize caecal microflora, and enhance the anti-oxidative ability and immunological function of laying hens. The appropriate supplemental level of IMO in the diet of laying hens was 0.2%.［Chinese Journal of Animal Nutrition, 2011, 23（9）：1560 -1568］
WU Shugeng,GAO Chunqi,ZHANG Haijun,TIAN Fang,QI Guanghai
. Effect of Dietary Isomalto-oligosaccharide on Performance, Caecal Microflora and Immune Function of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2011
, 23(09)
: 1560
-1568
.
DOI: 10.3969/j.issn.1006-267x.2011.09.015
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