Effects of Dietary Selenoyeast and Vitamin E on Growth, Immunity and Disease Resistance of Sea Cucumbers (Apostichopus japonicus Selenka)

ZHANG Qin,MAI Kangsen,ZHANG Wenbing,MA Hongming,AI Qinghui,XU Wei,LIUFU Zhiguo

doi:10.3969/j.issn.1006-267x.2011.10.014

2011 , Vol. 23 >Issue 10: 1745 - 1755

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.10.014

Fish Nutrition

Effects of Dietary Selenoyeast and Vitamin E on Growth, Immunity and Disease Resistance of Sea Cucumbers (Apostichopus japonicus Selenka)

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(1. Key Laboratory of Mariculture, Education Ministry of China, Ocean University of China, Qingdao 266003, China;
2. Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai 536000, China)

Online published: 2011-10-24

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Abstract

This trial was conducted to investigate the effects of dietary selenoyeast and vitamin E on growth, immunity and disease resistance of sea cucumbers (Apostichopus japonicus Selenka). A total of 600 sea cucumbers with an average initial body weight of (3.52±0.08) g were fed with 8 practical diets according to a 2×4 factorial design: a basal diet as the control diet supplemented with 2 levels of vitamin E (0 and 250 mg/kg), crossed with 0, 100, 300 or 600 mg/kg selenoyeast for 8 weeks. There were 3 replicates per group with 35 sea cucumbers. The feeding trial results showed that dietary selenoyeast and vitamin E had significant influence on specific growth rate (SGR) (P<0.05), and the highest SGR of sea cucumbers was found in the group with the diet containing 600 mg/kg selenoyeast and 250 mg/kg vitamin E. In the groups with the diets containing 0 mg/kg vitamin E, phagocytosis activity, respiratory burst (RB) activity, phenoloxidase (PO) activity and acid phosphatase (ACP) activity were increased with the selenoyeast level increasing, and above indices in the group with the diet containing 600 mg/kg selenoyeast were significantly higher than those in the control group (P<0.05). In the groups with the diets containing 250 mg/kg vitamin E, sea cucumbers fed the diet containing 600 mg/kg selenoyeast had significantly higher phagocytosis, RB activity and PO activity compared with the control group (P<0.05). The challenge trial results showed that dietary selenoyeast and vitamin E had significant effects on cumulative mortality at 14 days after infection with V. splendidus (P<0.05). When diets did not contain vitamin E, the cumulative mortality showed a decreasing tendency with selenoyeast increasing (P>0.05). When diets supplemented with 250 mg/kg vitamin E, sea cucumbers fed each selenoyeast level diet had significantly lower cumulative mortality compared with the control group (P<0.05). It can be concluded that the supplementation of selenoyeast and vitamin E in diets can enhance the SGR, immunity and disease resistance of sea cucumbers. Accordingly, the supplementation of 600 mg/kg selenoyeast and 250 mg/kg vitamin E in the diet is the optimal combination in the present study.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1745 -1755］

Key words： sea cucumbers (Apostichopus japonicas Selenka); ; selenoyeast; vitamin E; immunity; disease resistance

Cite this article

ZHANG Qin,MAI Kangsen,ZHANG Wenbing,MA Hongming,AI Qinghui,XU Wei,LIUFU Zhiguo . Effects of Dietary Selenoyeast and Vitamin E on Growth, Immunity and Disease Resistance of Sea Cucumbers (Apostichopus japonicus Selenka)[J]. Chinese Journal of Animal Nutrition, 2011 , 23(10) : 1745 -1755 . DOI: 10.3969/j.issn.1006-267x.2011.10.014

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