The study was conducted to investigate gene expression regularity of the three kinds of β-defensins [β-defensins-1 (pBD-1), β-defensins-2 (pBD-2) and β-defensins (pBD-3)] in different segments of intestinal tract in Jinhua and Landrace pigs from new-born to 60 days of age, and to compare differences of β-defensins gene expression between the two breeds. Three heads of Jinhua pigs and Landrace pigs (new-born, 20, 40 and 60 days of age) were selected and slaughtered, respectively, and tissue samples of duodenum, jejunum, ileum and colon were collected for analysis of gene expression levels of pBD-1, pBD-2, pBD-3 by real-time PCR. The results showed as follows: 1) with the days of age increasing, pBD-1 and pBD-3 gene expression levels in intestine increased, and pBD-2 gene expression level in duodenum and jejunum increased, while that in ileum and colon tended to firstly decrease and then increase. 2) At 20, 40 and 60 days of age, gene expression levels of the three β-defensins in different segments of intestine in Jinhua pigs were higher than those in Landrace pigs, respectively. 3) gene expression level of pBD-1 in duodenum of Jinhua pigs was significantly higher than that of new-born and 20-day-age Landrace pigs (P＜0.01); at 60 days of age, gene expression level of pBD-1 in jejunum, ileum and colon of Jinhua pigs was significantly higher than that of Landrace pigs (P＜0.05), gene expression level of pBD-2 in duodenum, ileum and colon of Jinhua pigs was significantly higher than that of Landrace pigs (P＜0.05), and gene expression level of pBD-3 in duodenum (P＜0.05), jejunum (P＜0.05), ileum and colin (P＜0.01) of Jinhua pigs was significantly higher than that of Landrace pigs (P＜0.05). These results indicate that the gene expression levels of pBD-1and pBD-3 increase in the intestinal tract of pigs from birth to 60 days of age, and gene expression levels of the three β-defensins in Jinhua pigs are higher than those in Landrace pigs at 20, 40 and 60 days of age.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1762 -1768］
AN Sha,HAN Feifei,GAO Yanhua,LIU Yifan,WANG Yizhen
. Gene Expression Profile of β-Defensins in Intestinal Tract of Jinhua and Landrace Pigs[J]. Chinese Journal of Animal Nutrition, 2011
, 23(10)
: 1762
-1768
.
DOI: 10.3969/j.issn.1006-267x.2011.10.016
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