This experiment was conducted to study the protective effects of fish oil on intestinal mucosa injury of piglets challenged with lipopolysaccharide (LPS). Thirty-two crossbred (Duroc×Landrace×Large White) piglets weaned at (28±3) d of age with body weight of (8.91±0.74) kg were used in a 2×2 factorial design. The piglets were randomly assigned to 4 groups with 4 replicates per group and 2 piglets per replicate. The main factors consisted of diet (5% corn oil or 5% fish oil) and immunological challenge (LPS injection or physiological saline injection). The experiment lasted for 19 days. On day 19, half of piglets in every diet group were injected intraperitoneally with either 150 μg/kg BW of LPS or an equivalent amount of sterile saline. At 4 h after LPS challenge, the piglets were slaughtered to collect intestinal mucosa samples for analysis. The results showed as follows: 1) LPS challenge reduced the villus height and crypt depth in jejunum and ileum （P<0.05）. Fish oil supplementation improved villus height, crypt depth and the ratio of villus height to crypt depth in ileum （P<0.05）. 2) LPS challenge reduced the activities of lactase, sucrase and maltase in jejunum (P<0.05). The sucrase activity of piglets fed fish oil was improved in jejunum (P<0.05). The lactase activity of piglets fed fish oil and injected physiological saline was increased in ileum (P<0.05). 3) LPS challenge increased tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) contents in jejunum and ileum (P<0.05). Piglets fed fish oil had lower TNF-α level than piglets fed corn oil (P<0.05). Fish oil supplementation inhibited PGE2 contents in jejunum (P<0.10). 4) LPS challenge increased nuclear transcription factor-kappaB (NF-κB) protein expression in jejunum and ileum (P<0.05). Fish oil suppressed NF-κB protein expression in ileum (P<0.05). These results suggest that fish oil can alleviate intestine damage induced by LPS challenge. Additionally, the protective effects of fish oil on the intestine are associated with decreasing the production of intestinal pro-inflammatory parameters through inhibiting NF-κB expression.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1775 -1782］
WU Zhifeng,LIU Yulan,HONG Yu,ZHU Huiling,HOU Yongqing,DING Bingying
. Fish Oil Supplementation Protects against Intestinal Mucosa Injury Induced by Lipopolysaccharide Challenge in Piglets[J]. Chinese Journal of Animal Nutrition, 2011
, 23(10)
: 1775
-1782
.
DOI: 10.3969/j.issn.1006-267x.2011.10.018
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