The experiment was conducted to study the effects of different Lactobacillus forms on growth performance and small intestinal mucosa morphology of broiler chickens. A total of 240 one-day-old chickens were randomly assigned to 4 groups with 5 replicates per group and 12 chickens per replicate. Group Ⅰ was Lactobacillus solid preparation group (basal diet+0.2% L. graminis solid preparation); group Ⅱ was Lactobacillus liquid preparation group (basal diet+L. graminis liquid preparation); group Ⅲ was antibiotic group (basal diet+0.01% flavomycin); group Ⅳ was control group (basal diet). The experimental period was 14 days. The results showed as follows: compared with the antibiotic group and Lactobacillus solid preparation group, the average daily gain of broilers aged from 1 to 14 days in the Lactobacillus liquid preparation group was significantly increased (P<0.05); there was no significant difference in the ratio of feed to gain among all groups (P>0.05); the effect of liquid preparation was better than that of solid preparation. Lactobacillus preparation could increase the height of intestinal villus and decrease the depth of crypt to a certain extent, but only Lactobacillus liquid preparation had a significant effect (P＜0.05). No significant difference was observed in the thickness of intestinal wall in different Lactobacillus preparation groups (P>0.05); the intestinal villus height/crypt depth of broilers in liquid Lactobacillus preparation group was significantly higher than that in the control group and antibiotic group (P＜0.05), but there was no significant difference between the solid Lactobacillus preparation group, control group and antibiotic group (P>0.05). In conclusion, the supplementation of Lactobacillus preparation in the diet can increase the average daily gain, and improve small intestinal mucosa morphology of broiler chickens to a certain extent, such as an increase in the height of intestinal villus and a decrease in the depth of crypt. Furthermore, the effect of liquid preparation is better than that of solid preparation.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1812 -1818］
XU Zihan,XU Li
. Different Lactobacillus Forms Affect Growth Performance and Small Intestinal Mucosa Morphology of Broiler Chickens[J]. Chinese Journal of Animal Nutrition, 2011
, 23(10)
: 1812
-1818
.
DOI: 10.3969/j.issn.1006-267x.2011.10.023
［1］张刚.乳酸细菌——基础、技术和应用[M].北京:化学工业出版,2007:2-11.
[2]郭兴华.益生菌——基础与应用[M].北京:科学技术出版社,2002:259-265.
[3]袁玲.格氏乳杆菌制剂的制备及其在肉雏鸡中的应用研究[D].硕士学位论文.哈尔滨:东北农业大学,2010:1.
[4]陈馨.鸡源乳酸菌的筛选及其应用效果的研究[D].硕士学位论文.哈尔滨:东北农业大学，2006:35-36.
[5]王晶,许丽,袁玲,等.同源乳酸菌对雏鸡生长性能和盲肠菌群的影响[J].中国饲料,2009,15:24-26.
[6]韩正康.家畜营养生理学[M].北京:农业出版社,1991:16-17.
[7]KATOY, YU D, SCHWARTZ M Z. Glucagonlik peptide-2 enhances small intestinal absorptive function and mucosal mass in vivo[J]. Journal of Pediatric Surgery, 1999(34):18-20.
[8]罗克.家禽解剖学与组织学[M].福州:福建科学技术出版社,1983:139-145.
[9]倪耀娣,李睿文,鲁改如,等.微生态制剂对肉仔鸡小肠结构的影响[J].江西畜牧兽医杂志,2004(4):7-8.
[10]刘惠.中草药复合粗多糖对断奶仔猪肠道消化生理影响的研究[D].硕士学位论文.长沙:湖南农业大学,2007:44-46.
[11]成令忠.组织学[M].2版.北京:人民卫生出版社,1994.
[12]EDENS F W, PARKHURST C R, CASAS I A, et al. Principles of ex ovo competitive exclusion and in ovo administration of Lactobacillus reuteri[J]. Poultry Science, 1997(76):179-196.
[13]李诺.黄芪提取物对鸡生长发育及免疫功能的影响[D].硕士学位论文.北京:中国农业大学,2004.
[14]HAMPSON D J. Alterations in piglet small intestinal structure at weaning[J]. Research in Veterinary Science, 1986(40):32-40.
[15]易丹.合生素对肉鸭生长性能、血液生化指标和肠道生理学指标的影响[D].硕士学位论文.武汉:武汉工业学院,2008.
[16]李树鹏.黄芪多糖、益生菌合生元对雏鸡生长和免疫的影响[D].杨凌:西北农林科技大学,2005:44-46.
