Hainanmycin: Effects on Rumen Microbial Fermentation and Nitrogen Metabolism in Vitro

WANG Zhibo, ZHANG Yonggen, XIN Hangshu, LIU Wei, XIA Ke

doi:10.3969/j.issn.1006-267x.2011.12.025

Chinese Journal of Animal Nutrition >

2011 , Vol. 23 >Issue 12: 2210 - 2216

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.12.025

Molecular Nutrition

Hainanmycin: Effects on Rumen Microbial Fermentation and Nitrogen Metabolism in Vitro

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College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2011-06-27

Online published: 2011-12-14

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Abstract

The effects of hainanmycin on rumen microbial fermentation and ruminal nitrogen metabolism in vitro were investigated in this study. Four doses of hainanmycin were evaluated using a 24 h batch culture of rumen fluid in vitro, with a forage-to-concentrate diet ratio of 60∶40 (18% crude protein, 30% neutral detergent fiber, and 22% acid detergent fiber). The groups were as follows: control group (CON), 10 mg/L monensin group (MON, positive control group), and the four doses of hainanmycin groups (H0.1, H1.0, H10.0 and H100.0), i.e., 0.1, 1.0, 10.0 and 100.0 mg/L hainanmycin. After 24 h, the pH was immediately measured. Samples were collected and analyzed for concentrations of peptide, amino acids, ammonia nitrogen (NH₃-N) and volatile fatty acids (VFA), activities of protease and deaminase, and DNA isolation. The results showed that H100.0 decreased the total VFA concentration and increased the final pH (P<0.05). H10.0, H100.0 and MON reduced the acetate-to-propionate ratio, as well as the concentrations of acetate and butyrate, but increased the propionate concentration compared with CON (P<0.05). H0.1 and H1.0 had no significant effects on rumen microbial fermentation (P>0.05). H10.0, H100.0 and MON increased protease activity and concentrations of amino acids and peptide (P<0.05), but decreased NH₃-N concentration and deaminase activity (P<0.05). H10.0, H100.0 and MON also increased the relative population size (RPS) of Prevotella ruminicola and genus Prevotella, but reduced the RPS of protozoa (P<0.05). H10.0, H100.0 and MON had no significant effects on the RPS of Selenomonas ruminantium, Megasphaera elsdenii and Ruminobacter amylophilus (P>0.05), but significantly decreased the RPS of Butyrivibrio fibrisolvens (P<0.05). In terms of improving rumen fermentation and increasing non-ammonia nitrogen flow, appropriate dose of hainanmycin is 10.0 mg/L. Moreover, Hainanmycin exhibited an action similar to that of monensin on the mode of rumen fermentations. In addition, hainanmycin regulated nitrogen metabolism by changing the number of Prevotella and protozoa.

Key words： hainanmycin; dose; rumen fermentation; nitrogen metabolism

Cite this article

WANG Zhibo, ZHANG Yonggen, XIN Hangshu, LIU Wei, XIA Ke . Hainanmycin: Effects on Rumen Microbial Fermentation and Nitrogen Metabolism in Vitro[J]. Chinese Journal of Animal Nutrition, 2011 , 23(12) : 2210 -2216 . DOI: 10.3969/j.issn.1006-267x.2011.12.025

References

[1] DINIUS D A, SIMPSON M E, MARSH P B. Effect of monensin fed with forage on digestion and the ruminal ecosystem of steers[J]. Journal of Animal Science, 1976, 42(1):229-234.

[2] HANSON T L, KLOPFENSTEIN T. Monensin, protein source and protein levels for growing steers[J]. Journal of Animal Science, 1979, 48(3):474-479.

[3] POOS M I, HANSON T L, KLOPFENSTEIN T J. Monensin effects on diet digestibility, ruminal protein bypass and microbial protein synthesis[J]. Journal of Animal Science, 1979, 48(6):1516-1524.

[4] VAN NEVEL C J, DEMEYER D I. Effect of monensin on rumen metabolism in vitro[J]. Applied and Environmental Microbiology, 1977, 34(3):251-257.

[5] 黄兵.离子载体抗球虫新药——海南霉素[J].养禽与禽病防治,2002(5):16-18.

[6] 任明强,沈赞明,赵茹茜,等.不同浓度海南霉素对山羊瘤胃消化代谢的影响[J].南京农业大学学报,1998,21(3):76-79.

[7] 李炯明,庄苏,王恬,等.海南霉素对人工瘤胃体外发酵调控的影响[J].家畜生态学报,2007,28(1):41-46.

[8] 沈赞明,陈杰,任明强.海南霉素对山羊瘤胃内肽的保护作用[J].南京农业大学学报,2004,27(4):73-76.

[9] TILLEY J M A, TERRY R A. A two stage technique for the in vitro digestion of forage crops[J]. Grass and Forage Science, 1963, 18(2):104-111.

[10] MCDOUGALL E I. Studies on ruminant saliva. 1. The composition and output of sheep's saliva[J]. The Biochemical Journal, 1948, 43(1):99-109.

[11] JOUANY J P. Volatile fatty acids and alcohol determination in digestive contents, silage juice, bacterial cultures and anaerobic fermentor contents[J]. Sciences des Aliments, 1982, 12(2):131-144.

[12] BRODERICK G A, KANG J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science, 1980, 63(1):64-75.

[13] MOORE S. Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction[J]. Journal of Animal Science, 1968, 243(23):6281-6283.

[14] SIDDONS R C, PARADINE J. Effect of diet on protein degrading activity in the sheep rumen[J]. Journal of the Science of Food and Agriculture, 1981, 32(10):973-981.

[15] STEVENSON D, WEIMER P. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR[J]. Applied Microbiology and Biotechnology, 2007, 75(1):165-174.

[16] BUSQUET M, CALSAMIGLIA S, FERRET A, et al. Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture[J]. Journal of Dairy Science, 2005, 88(7):2508-2516.

[17] RUSSELL J B, STROBEL H J. Effects of additives on in vitro ruminal fermentation: a comparison of monensin and bacitracin, another gram-positive antibiotic[J]. Journal of Animal Science, 1988, 66(2):552-558.

[18] YANG C M, RUSSELL J B. The effect of monensin supplementation on ruminal ammonia accumulation in vivo and the numbers of amino acid-fermenting bacteria[J]. Journal of Animal Science, 1993, 71(12):3470-3476.

[19] WEIMER P J, STEVENSON D M, MERTENS D R, et al. Effect of monensin feeding and withdrawal on populations of individual bacterial species in the rumen of lactating dairy cows fed high-starch rations[J]. Applied Microbiology and Biotechnology, 2008, 80(1):135-145.

[20] 沈向真,朱祖康,陆天水,等.海南霉素和半胱胺对水牛瘤胃消化代谢与增重的影响[J].江苏农业科学,2003(5):78-82.

[21] IVES S E, TITGEMEYER E C, NAGARAJA T G, et al. Effects of virginiamycin and monensin plus tylosin on ruminal protein metabolism in steers fed corn-based finishing diets with or without wet corn gluten feed[J]. Journal of Animal Science, 2002, 80(11):3005-3015.

[22] NEWBOLD C J, WALLACE R J, MCKAIN N. Effects of the ionophore tetronasin on nitrogen metabolism by ruminal microorganisms in vitro[J]. Journal of Animal Science, 1990, 68(4):1103-1109.

[23] HINO T, RUSSELL J B. Effect of reducing-equivalent disposal and NADH/NAD on deamination of amino acids by intact rumen microorganisms and their cell extracts[J]. Applied and Environmental Microbiology, 1985, 50(6):1368-1374.

[24] WALLACE R J, BRODERICK G A, BRAMMALL M L. Microbial protein and peptide metabolism in rumen fluid from faunated and ciliate-free sheep[J]. British Journal of Nutrition, 1987, 58(1):87-93.

[25] JOUANY J P. Effect of rumen protozoa on nitrogen utilization by ruminants[J]. The Journal of Nutrition, 1996, 126(Suppl. 4):1335S-1346S.

[26] ZINN R A, PLASCENCIA A, BARAJAS R. Interaction of forage level and monensin in diets for feedlot cattle on growth performance and digestive function[J]. Journal of Animal Science, 1994, 72(9):2209-2215.

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