This experiment was conducted to investigate the effects of gossypol-acetic acid on growth performance, serum biochemical indices and gossypol residue of Carassius auratus gibelio. A total of 270 Carassius auratus gibelio with an average body weight of (31.68±0.01) g were randomly divided into 3 groups with 3 replicates per group and 30 fish per replicate. Fish in the 3 groups were fed the basal diet supplemented with 0 (control group), 300 or 900 mg/kg gossypol-acetic acid, respectively. The experiment lasted for 8 weeks. The results showed as follows: with the increasing of gossypol-acetic acid supplementation, the growth performance of fish showed a decreasing tendency. Compared with the control group, the weight gain rate of fish in the 2 groups with the diet supplemented with 300 and 900 mg/kg gossypol-acetic acid, respectively, was decreased by 8.43% (P<0.05) and 12.22% (P<0.05), and the special growth rate was decreased by 5.82% (P<0.05) and 7.94% (P<0.05), while the feed conversion rate was increased by 7.69% (P>0.05) and 14.69% (P<0.05), respectively. With the increasing of gossypol-acetic acid supplementation, the condition factor and viscerosomatic index of fish were gradually decreased, while the hepaticsomatic index was gradually increased. The condition factor and hepaticsomatic index of fish in the group with the diet supplemented with 900 mg/kg gossypol-acetic acid showed significant differences when compared with the control group (P<0.05). The body length to body height ratio and the contents of moisture, ash, crude protein and crude lipid in muscle of fish were not influenced by gossypol-acetic acid supplementation (P>0.05). The activities of glutamic oxaolacetic transaminase and glutamic pyruvic transaminase, and creatinine content in serum of fish in the group with the diet supplemented with 900 mg/kg gossypol-acetic acid were significantly higher than those in the control group (P<0.05), while the alkaline phosphatase activity and total protein content in serum of fish in the group with the diet supplemented with 900 mg/kg gossypol-acetic acid were significantly lower than those in the control group (P<0.05). No significant differences were found in the contents of urea, total cholesterol, triglyceride, total bilirubin, glucose and calcium in serum among all groups (P>0.05). The free gossypol content in muscle and liver was significantly increased with the increasing of gossypol-acetic acid supplementation (P<0.05). In conclusion, when dietary gossypol-acetic acid supplementation reaches 300 mg/kg, the growth performance of Carassius auratus gibelio is decreased, and the gossypol residue in tissue is increased. Furthermore, when dietary gossypol-acetic acid supplementation reaches 900 mg/kg, the liver function and renal function of Carassius auratus gibelio are damaged.