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Juvenile Jian Carp (Cyprinus carpio var. Jian) with Phenylalanine Deficiency: Observation of Histopathological Changes and Antioxidant Ability

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  • 1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China;
    2. Key Laboratory of Animal Disease-Resistance Nutrition of Sichuan Province, Ya'an 625014, China;
    3. University Key Laboratory of Fish Nutrition and Safety Production of Sichuan Province, Ya'an 625014, China

Received date: 2011-07-22

  Online published: 2012-01-13

Abstract

This experiment was conducted to study the effects of phenylalanine deficiency on histopathological changes and antioxidant ability of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 400 juvenile Jian carp with an average body weight of (7.53±0.01) g were randomly divided into 2 groups with 4 replicates per group and 50 fish per replicate, and the fish in the 2 groups were fed with diets containing 1.9 (deficiency group) and 10.9 g/kg (control group) phenylalanine, respectively. The experiment lasted for 60 days. The results showed as follows: phenylalanine deficiency resulted in juvenile Jian carp tailfin upsweep; mortification and exfoliation of intestinal epithelium cell, and hydrops of lamina propria; vacuolar degeneration of hepatocyte; swelling of pancreatic gland cell and zymogen granule reduction in pancreatic gland acinus; ferrugination ferroheme deposition of spleen; carcoplasm solidification, dissolution or fragmentation of muscle fiber; renal glomerulus hyperemia and intumescence of hind kidney, and lumens slightly disfiguration; blood capillary hyperemia, dissolution and exfoliation as well as cartilage disappear of gill flake. Moreover, lipid peroxidation and protein oxidation were increased, and the activities of antioxidant enzymes, glutathione content and free radical-scavenging ability in serum were decreased. The results indicate that phenylalanine deficiency can induce pathological injure of tissues and organs at different degree, as well as decrease antioxidant ability of juvenile Jian carp.

Cite this article

ZENG Ting, FENG Lin, LIU Yang, JIANG Jun, ZHOU Xiaoqiu . Juvenile Jian Carp (Cyprinus carpio var. Jian) with Phenylalanine Deficiency: Observation of Histopathological Changes and Antioxidant Ability[J]. Chinese Journal of Animal Nutrition, 2012 , 24(1) : 183 -190 . DOI: 10.3969/j.issn.1006-267x.2012.01.026

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